KLHL7 ORF clone pFN21AE4151

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4151

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Clone info.

Species

human

Clone Name

pFN21AE4151 Sequence (with vector) Restriction Map

Product ID

FHC12646

ORF Sequence

DNA sequence (1758 bp)
Protein sequence (586 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4151

Symbol

KLHL7

Alias : CISS3, KLHL6, PERCHING, SBBI26

Description

kelch-like family member 7, transcript variant 1

Original Clone ID

cp94151

Note

We obtained this clone using the E.coli strain JM109.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1758 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 586 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS34609.1 0 100.0 KLHL7 CCDS5378.2 0 100.0 KLHL7 CCDS4192.1 4e-62 33.8 KLHL3 CCDS58970.1 4.4e-62 34.9 KLHL3 CCDS34094.1 1.1e-61 32.5 KLHL2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013069 34 140 PF00651 BTB/POZ IPR011705 146 248 PF07707 BTB/Kelch-associated IPR006652 326 363 PF01344 Kelch repeat type 1 IPR006652 371 416 PF01344 Kelch repeat type 1 IPR006652 420 467 PF01344 Kelch repeat type 1 IPR006652 470 515 PF01344 Kelch repeat type 1 HMMSmart IPR000210 44 141 SM00225 BTB/POZ-like IPR011705 146 248 SM00875 BTB/Kelch-associated IPR006652 337 382 SM00612 Kelch repeat type 1 IPR006652 383 430 SM00612 Kelch repeat type 1 IPR006652 431 481 SM00612 Kelch repeat type 1 IPR006652 482 528 SM00612 Kelch repeat type 1 ProfileScan IPR000210 44 111 PS50097 BTB/POZ-like
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 518 GVTVKCAAVGSIVYVLAGFQGVG 540 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001026880 586 611119,612943,617055 100.0 100.0 XP_016867928 564 611119,612943,617055 99.8 93.3 NP_061334 538 611119,612943,617055 100.0 91.8 XP_006715819 551 611119,612943,617055 97.6 91.8 XP_006715817 577 611119,612943,617055 97.5 93.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4151 Download

>KIEE4151  1758 bp
ATGGCAGCCTCTGGGGTGGAGAAGAGCAGCAAGAAGAAGACCGAGAAGAAACTTGCTGCT
CGGGAAGAAGCTAAATTGTTGGCGGGTTTCATGGGCGTCATGAATAACATGCGGAAACAG
AAAACGTTGTGTGACGTGATCCTCATGGTCCAGGAAAGAAAGATACCTGCTCATCGTGTT
GTTCTTGCTGCAGCCAGTCATTTTTTTAACTTAATGTTCACAACTAACATGCTTGAATCA
AAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACCTGATATTATTGAACAACTGGTGGAA
TTTGCTTATACTGCTAGAATTTCCGTGAATAGCAACAATGTTCAGTCTTTGTTGGATGCA
GCAAACCAATATCAGATTGAACCTGTGAAGAAAATGTGTGTTGATTTTTTGAAAGAACAA
GTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCTAGCGGAGTGTCTAGATTGTCCTGAA
TTGAAAGCAACTGCAGATGACTTTATTCATCAGCACTTTACTGAAGTTTACAAAACTGAT
GAATTTCTTCAACTTGATGTCAAGCGAGTAACACATCTTCTCAACCAGGACACTCTGACT
GTGAGAGCAGAGGATCAGGTTTATGATGCTGCAGTCAGGTGGTTGAAATACGATGAACCT
AATCGCCAGCCATTTATGGTTGATATCCTTGCTAAAGTCAGGTTTCCTCTTATATCAAAG
AATTTCTTAAGTAAAACGGTACAAGCTGAACCACTTATTCAAGACAATCCTGAATGCCTT
AAGATGGTGATAAGTGGAATGAGGTACCATCTACTGTCTCCAGAGGACCGAGAAGAACTT
GTAGATGGCACAAGACCTAGAAGAAAGAAACATGACTACCGCATAGCCCTATTTGGAGGC
TCTCAACCACAGTCTTGTAGATATTTTAACCCAAAGGATTATAGCTGGACAGACATCCGC
TGCCCCTTTGAAAAACGAAGAGATGCAGCATGCGTGTTTTGGGACAATGTAGTATACATT
TTGGGAGGCTCTCAGCTTTTCCCAATAAAGCGAATGGACTGCTATAATGTAGTGAAGGAT
AGCTGGTATTCGAAACTGGGTCCTCCGACACCTCGAGACAGCCTTGCTGCATGTGCTGCA
GAAGGCAAAATTTATACATCTGGAGGTTCAGAAGTAGGAAACTCAGCTCTGTATTTATTT
GAGTGCTATGATACGAGAACTGAAAGCTGGCACACAAAGCCCAGCATGCTGACCCAGCGC
TGCAGCCATGGGATGGTGGAAGCCAATGGCCTAATCTATGTTTGTGGTGGAAGTTTAGGA
AACAATGTTTCTGGGAGAGTGCTTAATTCCTGTGAAGTTTATGATCCTGCCACAGAAACA
TGGACTGAGCTGTGTCCAATGATTGAAGCCAGGAAGAATCATGGGCTGGTATTTGTAAAA
GACAAGATATTTGCTGTGGGTGGTCAGAATGGTTTAGGTGGTCTGGACAATGTGGAATAT
TACGATATTAAGTTGAACGAATGGAAGATGGTCTCACCAATGCCATGGAAGGGTGTAACA
GTGAAATGTGCAGCAGTTGGCTCTATAGTTTATGTCTTGGCTGGTTTTCAGGGTGTTGGT
CGATTAGGACACATTCTCGAATATAATACCGAAACAGACAAATGGGTTGCCAACTCCAAA
GTTCGTGCTTTTCCAGTCACAAGTTGTTTAATTTGTGTTGTCGATACTTGTGGAGCAAAT
GAAGAGACCCTTGAAACA

ORF Protein

Cloned ORF protein sequence for pFN21AE4151 Download

>KIEE4151  586 aa
MAASGVEKSSKKKTEKKLAAREEAKLLAGFMGVMNNMRKQKTLCDVILMVQERKIPAHRV
VLAAASHFFNLMFTTNMLESKSFEVELKDAEPDIIEQLVEFAYTARISVNSNNVQSLLDA
ANQYQIEPVKKMCVDFLKEQVDASNCLGISVLAECLDCPELKATADDFIHQHFTEVYKTD
EFLQLDVKRVTHLLNQDTLTVRAEDQVYDAAVRWLKYDEPNRQPFMVDILAKVRFPLISK
NFLSKTVQAEPLIQDNPECLKMVISGMRYHLLSPEDREELVDGTRPRRKKHDYRIALFGG
SQPQSCRYFNPKDYSWTDIRCPFEKRRDAACVFWDNVVYILGGSQLFPIKRMDCYNVVKD
SWYSKLGPPTPRDSLAACAAEGKIYTSGGSEVGNSALYLFECYDTRTESWHTKPSMLTQR
CSHGMVEANGLIYVCGGSLGNNVSGRVLNSCEVYDPATETWTELCPMIEARKNHGLVFVK
DKIFAVGGQNGLGGLDNVEYYDIKLNEWKMVSPMPWKGVTVKCAAVGSIVYVLAGFQGVG
RLGHILEYNTETDKWVANSKVRAFPVTSCLICVVDTCGANEETLET

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4151 Download

>pFN21AE4151  6463 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGCAGCCTCTGGGGTGGAGAAGAGCAGCAAGAA
GAAGACCGAGAAGAAACTTGCTGCTCGGGAAGAAGCTAAATTGTTGGCGGGTTTCATGGG
CGTCATGAATAACATGCGGAAACAGAAAACGTTGTGTGACGTGATCCTCATGGTCCAGGA
AAGAAAGATACCTGCTCATCGTGTTGTTCTTGCTGCAGCCAGTCATTTTTTTAACTTAAT
GTTCACAACTAACATGCTTGAATCAAAGTCCTTTGAAGTAGAACTCAAAGATGCTGAACC
TGATATTATTGAACAACTGGTGGAATTTGCTTATACTGCTAGAATTTCCGTGAATAGCAA
CAATGTTCAGTCTTTGTTGGATGCAGCAAACCAATATCAGATTGAACCTGTGAAGAAAAT
GTGTGTTGATTTTTTGAAAGAACAAGTTGATGCTTCAAATTGTCTTGGTATAAGTGTGCT
AGCGGAGTGTCTAGATTGTCCTGAATTGAAAGCAACTGCAGATGACTTTATTCATCAGCA
CTTTACTGAAGTTTACAAAACTGATGAATTTCTTCAACTTGATGTCAAGCGAGTAACACA
TCTTCTCAACCAGGACACTCTGACTGTGAGAGCAGAGGATCAGGTTTATGATGCTGCAGT
CAGGTGGTTGAAATACGATGAACCTAATCGCCAGCCATTTATGGTTGATATCCTTGCTAA
AGTCAGGTTTCCTCTTATATCAAAGAATTTCTTAAGTAAAACGGTACAAGCTGAACCACT
TATTCAAGACAATCCTGAATGCCTTAAGATGGTGATAAGTGGAATGAGGTACCATCTACT
GTCTCCAGAGGACCGAGAAGAACTTGTAGATGGCACAAGACCTAGAAGAAAGAAACATGA
CTACCGCATAGCCCTATTTGGAGGCTCTCAACCACAGTCTTGTAGATATTTTAACCCAAA
GGATTATAGCTGGACAGACATCCGCTGCCCCTTTGAAAAACGAAGAGATGCAGCATGCGT
GTTTTGGGACAATGTAGTATACATTTTGGGAGGCTCTCAGCTTTTCCCAATAAAGCGAAT
GGACTGCTATAATGTAGTGAAGGATAGCTGGTATTCGAAACTGGGTCCTCCGACACCTCG
AGACAGCCTTGCTGCATGTGCTGCAGAAGGCAAAATTTATACATCTGGAGGTTCAGAAGT
AGGAAACTCAGCTCTGTATTTATTTGAGTGCTATGATACGAGAACTGAAAGCTGGCACAC
AAAGCCCAGCATGCTGACCCAGCGCTGCAGCCATGGGATGGTGGAAGCCAATGGCCTAAT
CTATGTTTGTGGTGGAAGTTTAGGAAACAATGTTTCTGGGAGAGTGCTTAATTCCTGTGA
AGTTTATGATCCTGCCACAGAAACATGGACTGAGCTGTGTCCAATGATTGAAGCCAGGAA
GAATCATGGGCTGGTATTTGTAAAAGACAAGATATTTGCTGTGGGTGGTCAGAATGGTTT
AGGTGGTCTGGACAATGTGGAATATTACGATATTAAGTTGAACGAATGGAAGATGGTCTC
ACCAATGCCATGGAAGGGTGTAACAGTGAAATGTGCAGCAGTTGGCTCTATAGTTTATGT
CTTGGCTGGTTTTCAGGGTGTTGGTCGATTAGGACACATTCTCGAATATAATACCGAAAC
AGACAAATGGGTTGCCAACTCCAAAGTTCGTGCTTTTCCAGTCACAAGTTGTTTAATTTG
TGTTGTCGATACTTGTGGAGCAAATGAAGAGACCCTTGAAACAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT