################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII AccII AccIII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstPI BstUI BstYI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FbaI FseI FspI HapII HincII HindII HindIII HpaI HpaII KasI KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI PacI PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 803 GGTACC G^GTACC AccI 1012 GTATAC GT^[AC][GT]AC AclI 123 AACGTT AA^CGTT AcyI 94 GGCGTC G[GA]^CG[CT]C AlwNI 5 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1256 AGCGCT AGC^GCT Asp700I 541 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 803 GGTACC G^GTACC BanI 803 GGTACC G^G[CT][GA]CC BbsI 36 GAAGAC GAAGAC(2/6) BcgI 1051 CGAATGGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 36 GAAGAC GAAGAC(2/6) BsaHI 94 GGCGTC G[GA]^CG[CT]C BsgI 1568 GTGCAG GTGCAG(16/14) BsmFI 1001 GGGAC GGGAC(10/14) BsmI 772 GAATGC GAATGC(1/-1) BspHI 98 TCATGA T^CATGA BspMI 165 ACCTGC ACCTGC(4/8) Bst1107I 1012 GTATAC GTA^TAC BstAPI 1129 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1089 TTCGAA TT^CGAA BstXI 1537 CCAATGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1012 GTATAC GTA^TAC CfoI 1257 GCGC GCG^C DraII 1098 GGGTCCT [GA]G^G[ACGT]CC[CT] Eco47III 1256 AGCGCT AGC^GCT EcoO109I 1098 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 682 GATATC GAT^ATC FauI 82 GCGGG CCCGC(4/6) HaeII 1256 AGCGCT [GA]GCGC^[CT] HaeIII 1288 GGCC GG^CC HgaI 95 GCGTC GACGC(5/10) HhaI 1257 GCGC GCG^C Hin1I 94 GGCGTC G[GA]^CG[CT]C HinP1I 1257 GCGC G^CGC KpnI 803 GGTACC GGTAC^C MspA1I 957 CCGCTG C[AC]G^C[GT]G NheI 453 GCTAGC G^CTAGC NspV 1089 TTCGAA TT^CGAA PaeR7I 1112 CTCGAG C^TCGAG PflMI 1266 CCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1098 GGGTCCT [GA]G^G[AT]CC[CT] Psp1406I 123 AACGTT AA^CGTT RcaI 98 TCATGA T^CATGA SapI 21 GAAGAGC GCTCTTC(1/4) SfuI 1089 TTCGAA TT^CGAA SspI 1496 AATATT AAT^ATT TspRI 1559 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 1266 CCATGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 465 TCTAGA T^CTAGA XhoI 1112 CTCGAG C^TCGAG XmnI 541 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 739 GTAC GT^AC 804 GTAC AflII 726 CTTAAG C^TTAAG 778 CTTAAG AvaI 59 CTCGGG C^[CT]CG[GA]G 1112 CTCGAG BfrI 726 CTTAAG C^TTAAG 778 CTTAAG BpmI 818 CTCCAG CTGGAG(16/14) 1160 CTGGAG BsaI 1744 GAGACC GGTCTC(1/5) 1530 GGTCTC BsaJI 1266 CCATGG C^C[ACGT][ACGT]GG 1543 CCATGG BsoBI 59 CTCGGG C^[CT]CG[GA]G 1112 CTCGAG DsaI 1266 CCATGG C^C[GA][CT]GG 1543 CCATGG EcoT14I 1266 CCATGG C^C[AT][AT]GG 1543 CCATGG HinfI 235 GAATC G^A[ACGT]TC 1416 GAATC HphI 786 GGTGA GGTGA(8/7) 1534 TCACC MaeII 124 ACGT A^CGT 134 ACGT NcoI 1266 CCATGG C^CATGG 1543 CCATGG NlaIV 803 GGTACC GG[ACGT]^[ACGT]CC 1098 GGGTCC RsaI 739 GTAC GT^AC 804 GTAC SphI 988 GCATGC GCATG^C 1244 GCATGC StyI 1266 CCATGG C^C[AT][AT]GG 1543 CCATGG TfiI 235 GAATC G^A[AT]TC 1416 GAATC Tsp45I 131 GTGAC ^GT[GC]AC 1696 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AvaII BbvI BfaI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NspI PstI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI Tru9I TseI Tsp509I TthHB8I