(Linear) (Six-base) MAPSORT of: KIEE4151.seq Check: 2713 from: 1 to: 1758 KIEE4151.orf With 182 enzymes: SgfI * July 3, 2012 18:40 .. AccI GT'mk_AC Cuts at: 0 1013 1758 Size: 1013 745 Acc65I G'GTAC_C Cuts at: 0 803 1758 Size: 803 955 AclI AA'CG_TT Cuts at: 0 124 1758 Size: 124 1634 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 1758 Size: 542 1216 AfeI AGC'GCT Cuts at: 0 1258 1758 Size: 1258 500 AflII C'TTAA_G Cuts at: 0 726 778 1758 Size: 726 52 980 Fragments arranged by size: 980 726 52 AlwNI CAG_nnn'CTG Cuts at: 0 10 1758 Size: 10 1748 ApoI r'AATT_y Cuts at: 0 298 317 541 720 1148 1758 Size: 298 19 224 179 428 610 Fragments arranged by size: 610 428 298 224 179 19 AvaI C'yCGr_G Cuts at: 0 59 1112 1758 Size: 59 1053 646 Fragments arranged by size: 1053 646 59 BanI G'GyrC_C Cuts at: 0 803 1758 Size: 803 955 BbsI GAAGACnn'nnnn_ Cuts at: 0 43 1758 Size: 43 1715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1040 1074 1758 Size: 1040 34 684 Fragments arranged by size: 1040 684 34 BmgBI CAC'GTC Cuts at: 0 135 1758 Size: 135 1623 BmrI ACTGGGnnnn_n' Cuts at: 0 1105 1758 Size: 1105 653 BmtI G_CTAG'C Cuts at: 0 457 1758 Size: 457 1301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 803 1181 1758 Size: 803 378 577 Fragments arranged by size: 803 577 378 BsaI GGTCTCn'nnnn_ Cuts at: 0 1536 1738 1758 Size: 1536 202 20 Fragments arranged by size: 1536 202 20 BsaHI Gr'CG_yC Cuts at: 0 95 1758 Size: 95 1663 BseYI C'CCAG_C Cuts at: 0 1240 1253 1758 Size: 1240 13 505 Fragments arranged by size: 1240 505 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1589 1758 Size: 1589 169 BsmI GAATG_Cn' Cuts at: 0 778 1758 Size: 778 980 BspHI T'CATG_A Cuts at: 0 98 1758 Size: 98 1660 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 174 1758 Size: 174 1584 BstAPI GCAn_nnn'nTGC Cuts at: 0 1135 1758 Size: 1135 623 BstBI TT'CG_AA Cuts at: 0 1090 1758 Size: 1090 668 BstXI CCAn_nnnn'nTGG Cuts at: 0 1544 1758 Size: 1544 214 BstZ17I GTA'TAC Cuts at: 0 1014 1758 Size: 1014 744 BtgI C'CryG_G Cuts at: 0 1266 1543 1758 Size: 1266 277 215 Fragments arranged by size: 1266 277 215 EarI CTCTTCn'nnn_ Cuts at: 0 16 972 1736 1758 Size: 16 956 764 22 Fragments arranged by size: 956 764 22 16 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 542 803 1181 1758 Size: 542 261 378 577 Fragments arranged by size: 577 542 378 261 EcoO109I rG'GnC_Cy Cuts at: 0 1099 1758 Size: 1099 659 EcoRV GAT'ATC Cuts at: 0 684 1758 Size: 684 1074 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 741 1758 Size: 741 1017 HaeII r_GCGC'y Cuts at: 0 1260 1758 Size: 1260 498 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 153 185 792 824 1619 1651 1758 Size: 153 32 607 32 795 32 107 Fragments arranged by size: 795 607 153 107 32 32 32 KpnI G_GTAC'C Cuts at: 0 807 1758 Size: 807 951 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 332 1129 1758 Size: 332 797 629 Fragments arranged by size: 797 629 332 MspA1I CmG'CkG Cuts at: 0 959 1758 Size: 959 799 NcoI C'CATG_G Cuts at: 0 1266 1543 1758 Size: 1266 277 215 Fragments arranged by size: 1266 277 215 NheI G'CTAG_C Cuts at: 0 453 1758 Size: 453 1305 NspI r_CATG'y Cuts at: 0 111 231 992 1133 1248 1758 Size: 111 120 761 141 115 510 Fragments arranged by size: 761 510 141 120 115 111 PflMI CCAn_nnn'nTGG Cuts at: 0 1272 1758 Size: 1272 486 PfoI T'CCnGG_A Cuts at: 0 149 1758 Size: 149 1609 PpuMI rG'GwC_Cy Cuts at: 0 1099 1758 Size: 1099 659 PstI C_TGCA'G Cuts at: 0 192 495 633 1140 1264 1758 Size: 192 303 138 507 124 494 Fragments arranged by size: 507 494 303 192 138 124 SapI GCTCTTCn'nnn_ Cuts at: 0 16 1758 Size: 16 1742 SfcI C'TryA_G Cuts at: 0 188 491 629 1136 1260 1583 1758 Size: 188 303 138 507 124 323 175 Fragments arranged by size: 507 323 303 188 175 138 124 SmlI C'TyrA_G Cuts at: 0 726 778 1112 1758 Size: 726 52 334 646 Fragments arranged by size: 726 646 334 52 SphI G_CATG'C Cuts at: 0 992 1248 1758 Size: 992 256 510 Fragments arranged by size: 992 510 256 SspI AAT'ATT Cuts at: 0 1498 1758 Size: 1498 260 StyI C'CwwG_G Cuts at: 0 1266 1543 1758 Size: 1266 277 215 Fragments arranged by size: 1266 277 215 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 55 842 1447 1758 Size: 55 787 605 311 Fragments arranged by size: 787 605 311 55 XbaI T'CTAG_A Cuts at: 0 465 1758 Size: 465 1293 XhoI C'TCGA_G Cuts at: 0 1112 1758 Size: 1112 646 XmnI GAAnn'nnTTC Cuts at: 0 545 1758 Size: 545 1213 Enzymes that do cut: AccI Acc65I AclI AcuI AfeI AflII AlwNI ApoI AvaI BanI BbsI BcgI BmgBI BmrI BmtI BpmI BsaI BsaHI BseYI BsgI BsmI BspHI BspMI BstAPI BstBI BstXI BstZ17I BtgI EarI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I KpnI MmeI MspA1I NcoI NheI NspI PflMI PfoI PpuMI PstI SapI SfcI SmlI SphI SspI StyI TaqII XbaI XhoI XmnI Enzymes that do not cut: AarI AatII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstEII BstYI Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TatI TstI Tth111I XcmI XmaI ZraI