(Circular) (Six-base) MAPSORT of: pFN21AE4151.seq Check: 3855 from: 1 to: 6463 pFN21AE4151 6463 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3018 3806 1937 Size: 1081 788 4594 Fragments arranged by size: 4594 1081 788 Acc65I G'GTAC_C Cuts at: 2808 3784 2808 Size: 976 5487 AclI AA'CG_TT Cuts at: 1563 2129 4590 4963 1563 Size: 566 2461 373 3063 Fragments arranged by size: 3063 2461 566 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2547 4526 5910 6178 1632 Size: 915 1979 1384 268 1917 Fragments arranged by size: 1979 1917 1384 915 268 AfeI AGC'GCT Cuts at: 3263 3263 Size: 6463 AflII C'TTAA_G Cuts at: 820 1017 2731 2783 820 Size: 197 1714 52 4500 Fragments arranged by size: 4500 1714 197 52 AflIII A'CryG_T Cuts at: 1203 5362 6339 1203 Size: 4159 977 1327 Fragments arranged by size: 4159 1327 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6463 AlwNI CAG_nnn'CTG Cuts at: 2015 4206 5778 2015 Size: 2191 1572 2700 Fragments arranged by size: 2700 2191 1572 ApaLI G'TGCA_C Cuts at: 4520 5676 4520 Size: 1156 5307 ApoI r'AATT_y Cuts at: 1493 2303 2322 2546 2725 3153 3772 3998 4150 Size: 810 19 224 179 428 619 226 152 Cuts at: 4150 6259 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 810 619 428 226 224 179 152 19 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 2064 3117 3788 1870 Size: 73 121 1053 671 4545 Fragments arranged by size: 4545 1053 671 121 73 BamHI G'GATC_C Cuts at: 3793 4160 3793 Size: 367 6096 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2808 3784 5239 618 Size: 325 217 627 1021 976 1455 1842 Fragments arranged by size: 1842 1455 1021 976 627 325 217 BanII G_rGCy'C Cuts at: 1391 3782 1391 Size: 2391 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 928 Size: 971 149 5343 Fragments arranged by size: 5343 971 149 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3045 3079 4654 4688 1903 Size: 34 1108 34 1575 34 3678 Fragments arranged by size: 3678 1575 1108 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4355 5565 4355 Size: 1210 5253 BclI T'GATC_A Cuts at: 1548 1548 Size: 6463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5074 136 Size: 107 122 71 631 605 3402 1525 Fragments arranged by size: 3402 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6458 6458 Size: 6463 BlpI GC'TnA_GC Cuts at: 3880 3880 Size: 6463 Bme1580I G_kGCm'C Cuts at: 1790 4524 5680 1790 Size: 2734 1156 2573 Fragments arranged by size: 2734 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2140 1318 Size: 822 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3110 4194 5152 Size: 416 53 83 399 24 1693 1084 958 Cuts at: 5152 442 Size: 1753 Fragments arranged by size: 1753 1693 1084 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2462 1056 Size: 1406 5057 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2808 3186 5123 1239 Size: 1569 378 1937 2579 Fragments arranged by size: 2579 1937 1569 378 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4588 5453 5751 4588 Size: 865 298 5300 Fragments arranged by size: 5300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3541 3743 5126 882 Size: 617 2042 202 1383 2219 Fragments arranged by size: 2219 2042 1383 617 202 BsaAI yAC'GTr Cuts at: 493 1204 4254 493 Size: 711 3050 2702 Fragments arranged by size: 3050 2702 711 BsaBI GATnn'nnATC Cuts at: 6457 6457 Size: 6463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 4270 4652 5287 Size: 53 83 186 936 567 2170 382 635 Cuts at: 5287 275 Size: 1451 Fragments arranged by size: 2170 1451 936 635 567 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4896 5294 5568 5715 6126 4896 Size: 398 274 147 411 5233 Fragments arranged by size: 5233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6238 Fragments arranged by size: 6238 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6463 BseYI C'CCAG_C Cuts at: 3245 3258 5666 6162 3245 Size: 13 2408 496 3546 Fragments arranged by size: 3546 2408 496 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3594 1163 Size: 573 1858 4032 Fragments arranged by size: 4032 1858 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4524 4609 5680 4524 Size: 85 1071 5307 Fragments arranged by size: 5307 1071 85 BsmI GAATG_Cn' Cuts at: 2783 3974 4067 2783 Size: 1191 93 5179 Fragments arranged by size: 5179 1191 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3782 4524 4609 5680 1391 Size: 399 1992 742 85 1071 2174 Fragments arranged by size: 2174 1992 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6126 6126 Size: 6463 BspHI T'CATG_A Cuts at: 2103 4352 6082 2103 Size: 2249 1730 2484 Fragments arranged by size: 2484 2249 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2179 3818 844 Size: 1335 1639 3489 Fragments arranged by size: 3489 1639 1335 BsrBI CCG'CTC Cuts at: 1394 1823 4350 1394 Size: 429 2527 3507 Fragments arranged by size: 3507 2527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4958 5132 65 Size: 1183 3710 174 1396 Fragments arranged by size: 3710 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5107 5294 1700 Size: 110 3297 187 2869 Fragments arranged by size: 3297 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 3140 794 Size: 2346 4117 BstBI TT'CG_AA Cuts at: 3095 5267 3095 Size: 2172 4291 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstXI CCAn_nnnn'nTGG Cuts at: 3549 3549 Size: 6463 BstYI r'GATC_y Cuts at: 1972 3793 4160 4545 4562 6014 6100 6112 6458 Size: 1821 367 385 17 1452 86 12 346 Cuts at: 6458 1972 Size: 1977 Fragments arranged by size: 1977 1821 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3019 3019 Size: 6463 BtgI C'CryG_G Cuts at: 513 1065 2004 3271 3548 513 Size: 552 939 1267 277 3428 Fragments arranged by size: 3428 1267 939 552 277 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 3979 4773 4793 3979 Size: 794 20 5649 Fragments arranged by size: 5649 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3910 4799 6428 Size: 54 1425 50 275 18 2080 889 1629 Cuts at: 6428 8 Size: 43 Fragments arranged by size: 2080 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2977 3741 4393 1353 Size: 668 956 764 652 3423 Fragments arranged by size: 3423 956 764 668 652 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5050 5424 5570 387 Size: 1064 138 3461 374 146 1280 Fragments arranged by size: 3461 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2547 2808 3186 4526 5123 5910 6178 Size: 393 915 261 378 1340 597 787 268 Cuts at: 6178 1239 Size: 1524 Fragments arranged by size: 1524 1340 915 787 597 393 378 268 261 EcoO109I rG'GnC_Cy Cuts at: 3104 3104 Size: 6463 EcoRI G'AATT_C Cuts at: 3772 4150 3772 Size: 378 6085 EcoRV GAT'ATC Cuts at: 2689 2689 Size: 6463 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2746 3770 2746 Size: 1024 5439 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 3265 5610 1678 Size: 1587 2345 2531 Fragments arranged by size: 2531 2345 1587 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2158 2190 2797 2829 3624 Size: 32 135 32 586 32 607 32 795 Cuts at: 3624 3656 5104 5136 5178 5210 1373 Size: 32 1448 32 42 32 2626 Fragments arranged by size: 2626 1448 795 607 586 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3807 4053 669 Size: 988 281 1869 246 3079 Fragments arranged by size: 3079 1869 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 4053 4053 Size: 6463 KpnI G_GTAC'C Cuts at: 2812 3788 2812 Size: 976 5487 MfeI C'AATT_G Cuts at: 4062 4062 Size: 6463 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 3134 5577 5761 1305 Size: 1032 797 2443 184 2007 Fragments arranged by size: 2443 2007 1032 797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4641 Fragments arranged by size: 4641 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4421 4780 4939 518 Size: 648 3255 359 159 2042 Fragments arranged by size: 3255 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2964 3879 4556 5704 5949 1606 Size: 1358 915 677 1148 245 2120 Fragments arranged by size: 2120 1358 1148 915 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3271 3548 513 Size: 552 939 1267 277 3428 Fragments arranged by size: 3428 1267 939 552 277 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NheI G'CTAG_C Cuts at: 1052 2458 1052 Size: 1406 5057 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 2116 2236 2997 3138 3253 3821 5366 Size: 97 887 120 761 141 115 568 1545 Cuts at: 5366 1132 Size: 2229 Fragments arranged by size: 2229 1545 887 761 568 141 120 115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6463 PciI A'CATG_T Cuts at: 5362 5362 Size: 6463 PflMI CCAn_nnn'nTGG Cuts at: 1108 3277 1108 Size: 2169 4294 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 6463 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4532 4564 6045 6077 4532 Size: 32 1481 32 4918 Fragments arranged by size: 4918 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3104 3104 Size: 6463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 4033 4033 Size: 6463 PstI C_TGCA'G Cuts at: 830 2197 2500 2638 3145 3269 3815 830 Size: 1367 303 138 507 124 546 3478 Fragments arranged by size: 3478 1367 546 507 303 138 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SapI GCTCTTCn'nnn_ Cuts at: 2021 2021 Size: 6463 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 4711 6423 4711 Size: 1712 4751 SfcI C'TryA_G Cuts at: 826 1045 2193 2496 2634 3141 3265 3588 3811 Size: 219 1148 303 138 507 124 323 223 Cuts at: 3811 4946 5627 5818 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1148 1135 681 507 323 303 223 219 191 138 124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3790 1872 Size: 1918 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2731 2783 3117 4567 5468 5730 Size: 197 926 788 52 334 1450 901 262 Cuts at: 5730 820 Size: 1553 Fragments arranged by size: 1553 1450 926 901 788 334 262 197 52 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 2997 3253 3821 1132 Size: 1865 256 568 3774 Fragments arranged by size: 3774 1865 568 256 SspI AAT'ATT Cuts at: 5 52 3503 4387 5 Size: 47 3451 884 2081 Fragments arranged by size: 3451 2081 884 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6463 StyI C'CwwG_G Cuts at: 513 1065 2004 3271 3548 3902 513 Size: 552 939 1267 277 354 3074 Fragments arranged by size: 3074 1267 939 552 354 277 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 2847 3452 4490 4507 4660 4845 Size: 1255 480 787 605 1038 17 153 185 Cuts at: 4845 5290 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1038 787 605 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4709 6421 96 Size: 274 80 33 51 1443 2732 1712 138 Fragments arranged by size: 2732 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6423 6455 6423 Size: 32 6431 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6463 XbaI T'CTAG_A Cuts at: 2470 3799 2470 Size: 1329 5134 XhoI C'TCGA_G Cuts at: 1943 3117 1943 Size: 1174 5289 XmaI C'CCGG_G Cuts at: 1870 3788 1870 Size: 1918 4545 XmnI GAAnn'nnTTC Cuts at: 2550 4592 2550 Size: 2042 4421 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI