RASGEF1B ORF clone pFN21AE4103

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4103

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Clone info.

Species

human

Clone Name

pFN21AE4103 Sequence (with vector) Restriction Map

Product ID

FHC12693

ORF Sequence

DNA sequence (1416 bp)
Protein sequence (472 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4103

Symbol

RASGEF1B

Alias : GPIG4

Description

RasGEF domain family, member 1B, transcript variant 2

Original Clone ID

cp94103

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1416 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 472 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS75152.1 6.2e-197 100.0 RASGEF1B CCDS34022.1 3.7e-196 99.8 RASGEF1B CCDS75151.1 8.7e-153 91.3 RASGEF1B CCDS4452.1 1.1e-128 68.0 RASGEF1C CCDS7202.2 3.3e-116 58.6 RASGEF1A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000651 40 135 PF00618 Ras-like guanine nucleotide exchange factor IPR001895 204 402 PF00617 Guanine-nucleotide dissociation stimulator CDC25 HMMSmart IPR000651 33 157 SM00229 Ras-like guanine nucleotide exchange factor IPR001895 200 453 SM00147 Guanine-nucleotide dissociation stimulator CDC25 ProfileScan IPR000651 34 164 PS50212 Ras-like guanine nucleotide exchange factor IPR001895 204 452 PS50009 Guanine-nucleotide dissociation stimulator CDC25
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001287664 472 614532 100.0 100.0 NP_689758 473 614532 99.8 100.0 XP_016863302 473 614532 99.8 100.0 NP_001287665 431 614532 91.3 100.0 XP_016863301 432 614532 91.1 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4103 Download

>KIEE4103  1416 bp
ATGCCTCAGACTCCTCCCTTTTCAGCAATGTTTGACAGCAGTGGTTACAATCGAAACCTC
TATCAGTCTGCAGAGGACAGCTGTGGAGGGTTGTATTACCATGACAACAACCTCCTCTCT
GGATCCCTGGAAGCACTCATCCAGCACTTAGTACCTAATGTGGATTACTATCCAGATAGA
ACATACATATTTACCTTCCTACTCAGTTCTCGGTTATTTATGCATCCGTATGAGCTAATG
GCCAAAGTTTGCCACTTATGTGTTGAGCACCAGAGACTAAGTGATCCTGATAGTGATAAG
AACCAGATGAGAAAAATTGCACCCAAAATCCTTCAACTCCTCACGGAATGGACGGAAACA
TTTCCCTATGATTTTCGGGATGAAAGAATGATGAGAAACTTAAAAGATCTGGCTCACCGA
ATAGCCAGTGGCGAAGAGACATACAGAAAGAATGTCCAGCAAATGATGCAGTGTCTGATC
CGCAAGCTTGCTGCGCTCAGCCAGTACGAAGAAGTCCTGGCAAAAATCAGCTCCACATCC
ACAGATCGGCTCACAGTTCTCAAGACCAAGCCACAGTCTATACAAAGGGATATCATTACT
GTCTGCAACGACCCTTACACGTTGGCCCAGCAGCTGACTCATATAGAGCTGGAGAGGCTC
AATTATATTGGGCCAGAAGAATTTGTTCAGGCGTTCGTGCAGAAGGACCCTTTGGATAAT
GACAAGAGTTGCTACAGTGAACGGAAGAAAACACGAAACTTAGAAGCTTACGTGGAATGG
TTTAATCGCCTCAGCTACTTGGTTGCTACAGAAATCTGTATGCCTGTTAAGAAAAAACAC
CGAGCAAGAATGATTGAGTATTTCATTGACGTAGCTCGGGAGTGTTTTAACATTGGCAAC
TTCAACTCCTTGATGGCGATAATCTCTGGTATGAATATGAGCCCAGTCTCTCGACTAAAA
AAAACTTGGGCCAAAGTGAAGACTGCAAAATTTGACATTCTTGAGCATCAGATGGACCCT
TCAAGCAATTTCTATAATTATCGAACAGCTCTTCGTGGGGCAGCACAAAGGTCTTTAACT
GCTCATAGTAGTAGAGAAAAGATTGTGATACCATTCTTCAGTCTCTTAATCAAAGATATT
TATTTCCTCAATGAGGGTTGTGCCAACCGCCTTCCCAATGGCCATGTCAATTTTGAGAAA
TTTTGGGAACTGGCCAAACAAGTGAGTGAATTTATGACATGGAAACAAGTGGAGTGTCCA
TTTGAGAGGGACCGGAAGATCTTGCAGTATCTGCTCACAGTACCAGTCTTCAGTGAAGAT
GCTCTCTACTTGGCTTCTTATGAGAGTGAAGGACCTGAAAATCATATAGAGAAAGACAGA
TGGAAGTCTTTAAGGTCGAGCCTCTTAGGCAGAGTT

ORF Protein

Cloned ORF protein sequence for pFN21AE4103 Download

>KIEE4103  472 aa
MPQTPPFSAMFDSSGYNRNLYQSAEDSCGGLYYHDNNLLSGSLEALIQHLVPNVDYYPDR
TYIFTFLLSSRLFMHPYELMAKVCHLCVEHQRLSDPDSDKNQMRKIAPKILQLLTEWTET
FPYDFRDERMMRNLKDLAHRIASGEETYRKNVQQMMQCLIRKLAALSQYEEVLAKISSTS
TDRLTVLKTKPQSIQRDIITVCNDPYTLAQQLTHIELERLNYIGPEEFVQAFVQKDPLDN
DKSCYSERKKTRNLEAYVEWFNRLSYLVATEICMPVKKKHRARMIEYFIDVARECFNIGN
FNSLMAIISGMNMSPVSRLKKTWAKVKTAKFDILEHQMDPSSNFYNYRTALRGAAQRSLT
AHSSREKIVIPFFSLLIKDIYFLNEGCANRLPNGHVNFEKFWELAKQVSEFMTWKQVECP
FERDRKILQYLLTVPVFSEDALYLASYESEGPENHIEKDRWKSLRSSLLGRV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4103 Download

>pFN21AE4103  6121 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCCTCAGACTCCTCCCTTTTCAGCAATGTTTGA
CAGCAGTGGTTACAATCGAAACCTCTATCAGTCTGCAGAGGACAGCTGTGGAGGGTTGTA
TTACCATGACAACAACCTCCTCTCTGGATCCCTGGAAGCACTCATCCAGCACTTAGTACC
TAATGTGGATTACTATCCAGATAGAACATACATATTTACCTTCCTACTCAGTTCTCGGTT
ATTTATGCATCCGTATGAGCTAATGGCCAAAGTTTGCCACTTATGTGTTGAGCACCAGAG
ACTAAGTGATCCTGATAGTGATAAGAACCAGATGAGAAAAATTGCACCCAAAATCCTTCA
ACTCCTCACGGAATGGACGGAAACATTTCCCTATGATTTTCGGGATGAAAGAATGATGAG
AAACTTAAAAGATCTGGCTCACCGAATAGCCAGTGGCGAAGAGACATACAGAAAGAATGT
CCAGCAAATGATGCAGTGTCTGATCCGCAAGCTTGCTGCGCTCAGCCAGTACGAAGAAGT
CCTGGCAAAAATCAGCTCCACATCCACAGATCGGCTCACAGTTCTCAAGACCAAGCCACA
GTCTATACAAAGGGATATCATTACTGTCTGCAACGACCCTTACACGTTGGCCCAGCAGCT
GACTCATATAGAGCTGGAGAGGCTCAATTATATTGGGCCAGAAGAATTTGTTCAGGCGTT
CGTGCAGAAGGACCCTTTGGATAATGACAAGAGTTGCTACAGTGAACGGAAGAAAACACG
AAACTTAGAAGCTTACGTGGAATGGTTTAATCGCCTCAGCTACTTGGTTGCTACAGAAAT
CTGTATGCCTGTTAAGAAAAAACACCGAGCAAGAATGATTGAGTATTTCATTGACGTAGC
TCGGGAGTGTTTTAACATTGGCAACTTCAACTCCTTGATGGCGATAATCTCTGGTATGAA
TATGAGCCCAGTCTCTCGACTAAAAAAAACTTGGGCCAAAGTGAAGACTGCAAAATTTGA
CATTCTTGAGCATCAGATGGACCCTTCAAGCAATTTCTATAATTATCGAACAGCTCTTCG
TGGGGCAGCACAAAGGTCTTTAACTGCTCATAGTAGTAGAGAAAAGATTGTGATACCATT
CTTCAGTCTCTTAATCAAAGATATTTATTTCCTCAATGAGGGTTGTGCCAACCGCCTTCC
CAATGGCCATGTCAATTTTGAGAAATTTTGGGAACTGGCCAAACAAGTGAGTGAATTTAT
GACATGGAAACAAGTGGAGTGTCCATTTGAGAGGGACCGGAAGATCTTGCAGTATCTGCT
CACAGTACCAGTCTTCAGTGAAGATGCTCTCTACTTGGCTTCTTATGAGAGTGAAGGACC
TGAAAATCATATAGAGAAAGACAGATGGAAGTCTTTAAGGTCGAGCCTCTTAGGCAGAGT
TGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T