Restriction map for
pFN21AE4103
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfaI BfrI BlnI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT14I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeI MfeI
MluI MroI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tsp45I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AflIII 617 ACACGT A^C[GA][CT]GT
AspHI 265 GAGCAC G[AT]GC[AT]^C
AvaI 875 CTCGGG C^[CT]CG[GA]G
BamHI 121 GGATCC G^GATCC
BanII 939 GAGCCC G[GA]GC[CT]^C
BbvCI 789 CCTCAGC CCTCAGC(-5/-2)
BglI 1400 GCCTCTTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 495 GCTCAGC GC^T[ACGT]AGC
BpmI 649 CTGGAG CTGGAG(16/14)
Bpu1102I 495 GCTCAGC GC^T[ACGT]AGC
BsaAI 769 TACGTG [CT]AC^GT[GA]
BsaBI 163 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 125 CCCTGG C^C[ACGT][ACGT]GG
BsaWI 1271 ACCGGA [AT]^CCGG[AT]
BsgI 697 GTGCAG GTGCAG(16/14)
BsiHKAI 265 GAGCAC G[AT]GC[AT]^C
BsmFI 1268 GGGAC GGGAC(10/14)
BsoBI 875 CTCGGG C^[CT]CG[GA]G
BsrDI 25 GCAATG GCAATG(2/0)
CelII 495 GCTCAGC GC^T[ACGT]AGC
CfoI 493 GCGC GCG^C
EcoNI 1146 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 589 GATATC GAT^ATC
EcoT22I 220 ATGCAT ATGCA^T
HapII 1272 CCGG C^CGG
HhaI 493 GCGC GCG^C
HinP1I 493 GCGC G^CGC
HpaII 1272 CCGG C^CGG
HphI 414 TCACC GGTGA(8/7)
MaeIII 44 GTTAC ^GT[ACGT]AC
MamI 163 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1272 CCGG C^CGG
NsiI 220 ATGCAT ATGCA^T
PstI 68 CTGCAG CTGCA^G
SapI 1048 GCTCTTC GCTCTTC(1/4)
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# TWO CUT ENZYMES ( 29 enzymes) #
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AciI 480 CCGC CCGC(-3/-1)
1167 CCGC
Asp700I 355 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
679 GAATTTGTTC
BbsI 978 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BglII 405 AGATCT A^GATCT
1277 AGATCT
BmyI 265 GAGCAC G[AGT]GC[ACT]^C
939 GAGCCC
BpuAI 978 GAAGAC GAAGAC(2/6)
1306 GTCTTC
BsiYI 539 CCACAGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1146 CCTCAATGAGG
BslI 539 CCACAGATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1146 CCTCAATGAGG
Bsp1286I 265 GAGCAC G[AGT]GC[ACT]^C
939 GAGCCC
BssKI 126 CCTGG ^CC[ACGT]GG
516 CCTGG
BstAPI 459 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1284 GCAGTATCTGC
BstNI 126 CCTGG CC^[AT]GG
516 CCTGG
Cac8I 482 GCAAGC GC[ACGT]^[ACGT]GC
486 GCTTGC
DraII 704 AGGACCC [GA]G^G[ACGT]CC[CT]
1350 AGGACCT
EarI 1049 CTCTTC CTCTTC(1/4)
433 GAAGAG
EcoO109I 704 AGGACCC [GA]G^G[ACGT]CC[CT]
1350 AGGACCT
EcoRII 126 CCTGG ^CC[AT]GG
516 CCTGG
HindIII 484 AAGCTT A^AGCTT
764 AAGCTT
HinfI 9 GACTC G^A[ACGT]TC
636 GACTC
Ksp632I 1049 CTCTTC CTCTTC(1/4)
433 GAAGAG
MslI 223 CATCCGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
253 CACTTATGTG
MspA1I 78 CAGCTG C[AC]G^C[GT]G
631 CAGCTG
MvaI 126 CCTGG CC^[AT]GG
516 CCTGG
PleI 9 GACTC GAGTC(4/5)
636 GACTC
PpuMI 704 AGGACCC [GA]G^G[AT]CC[CT]
1350 AGGACCT
PvuII 78 CAGCTG CAG^CTG
631 CAGCTG
ScrFI 126 CCTGG CC^[ACGT]GG
516 CCTGG
SmlI 559 CTCAAG C^T[CT][GA]AG
1000 CTTGAG
XmnI 355 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
679 GAATTTGTTC
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AvaII BalI BbvI BseRI
BsmAI BsrI BstF5I BstYI Cfr13I
DdeI DpnII EaeI Fnu4HI FokI
HaeIII ItaI MaeII MboI MboII
MflI MluNI MnlI MscI MseI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I SfaNI SfcI TaqI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII