REM2 ORF clone pFN21AE4063

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4063

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Clone info.

Species

human

Clone Name

pFN21AE4063 Sequence (with vector) Restriction Map

Product ID

FHC31674

ORF Sequence

DNA sequence (1020 bp)
Protein sequence (340 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4063

Symbol

REM2

Description

RAS (RAD and GEM)-like GTP binding 2

Original Clone ID

cp94063

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1020 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 340 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS45082.1 1.1e-108 100.0 REM2 CCDS10824.1 5.7e-35 49.0 RRAD CCDS6261.1 1.1e-32 45.5 GEM CCDS13181.1 1.8e-27 42.6 REM1 CCDS58037.1 7.5e-14 37.0 RIT1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 115 136 PR00449 Ras GTPase IPR001806 156 178 PR00449 Ras GTPase IPR001806 220 233 PR00449 Ras GTPase IPR001806 255 277 PR00449 Ras GTPase HMMPfam IPR013753 116 277 PF00071 Ras HMMSmart IPR003577 115 280 SM00173 Ras small GTPase IPR003579 115 280 SM00175 Ras small GTPase IPR003578 117 280 SM00174 Small GTPase ProfileScan IPR020849 109 340 PS51421 Ras small GTPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_775798 340 616955 100.0 100.0 XP_005267440 341 616955 99.7 100.0 XP_016876548 372 616955 100.0 90.0 XP_016876547 373 616955 99.7 90.0 NP_004156 308 179503 49.0 85.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4063 Download

>KIEE4063  1020 bp
ATGCACACGGACCTGGACACAGACATGGACATGGACACAGAAACCACAGCACTCTGCCCC
TCTGGCAGCCGCCGGGCCTCCCCTCCAGGGACGCCCACACCAGAAGCAGATGCCACGCTA
CTAAAGAAGTCAGAGAAACTGTTGGCAGAGTTGGACCGGAGCGGGTTACCCTCTGCCCCT
GGGGCCCCCAGACGAAGAGGCAGTATGCCTGTCCCCTACAAGCACCAGCTCCGGCGGGCC
CAGGCTGTAGATGAACTTGACTGGCCACCTCAGGCCTCATCCTCTGGCTCGTCTGACTCC
TTGGGCTCAGGGGAGGCAGCCCCTGCTCAAAAGGATGGCATCTTCAAGGTCATGCTAGTG
GGGGAGAGCGGCGTGGGCAAGAGCACCCTAGCAGGCACTTTTGGTGGTCTCCAGGGAGAC
AGTGCTCACGAACCGGAGAACCCAGAGGATACCTATGAGAGACGCATCATGGTGGATAAG
GAGGAAGTGACTCTAGTCGTTTATGACATCTGGGAACAGGGGGATGCAGGAGGGTGGCTG
CGGGACCACTGCCTTCAGACCGGGGACGCCTTTCTCATCGTCTTCTCAGTCACCGACCGA
CGGAGTTTCTCCAAAGTTCCAGAGACCCTACTTCGGCTCCGGGCTGGGAGGCCGCACCAC
GACCTACCCGTTATCCTCGTTGGAAACAAGAGCGACTTGGCCCGCTCCCGGGAGGTATCA
CTGGAGGAGGGCCGCCACCTGGCCGGGACGCTGAGCTGCAAGCACATCGAGACGTCGGCC
GCACTGCACCACAACACGAGGGAGCTCTTCGAGGGCGCGGTGCGCCAGATCCGGCTGCGG
CGGGGCCGAAACCACGCCGGAGGCCAGAGGCCCGATCCGGGCAGCCCCGAGGGCCCTGCG
CCACCTGCACGCCGCGAGAGCCTCACCAAGAAAGCCAAGAGGTTCCTCGCCAACCTGGTG
CCGCGCAACGCCAAGTTCTTCAAGCAGCGCTCCAGGTCGTGTCACGACCTCTCGGTGCTC

ORF Protein

Cloned ORF protein sequence for pFN21AE4063 Download

>KIEE4063  340 aa
MHTDLDTDMDMDTETTALCPSGSRRASPPGTPTPEADATLLKKSEKLLAELDRSGLPSAP
GAPRRRGSMPVPYKHQLRRAQAVDELDWPPQASSSGSSDSLGSGEAAPAQKDGIFKVMLV
GESGVGKSTLAGTFGGLQGDSAHEPENPEDTYERRIMVDKEEVTLVVYDIWEQGDAGGWL
RDHCLQTGDAFLIVFSVTDRRSFSKVPETLLRLRAGRPHHDLPVILVGNKSDLARSREVS
LEEGRHLAGTLSCKHIETSAALHHNTRELFEGAVRQIRLRRGRNHAGGQRPDPGSPEGPA
PPARRESLTKKAKRFLANLVPRNAKFFKQRSRSCHDLSVL

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4063 Download

>pFN21AE4063  5725 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCACACGGACCTGGACACAGACATGGACATGGA
CACAGAAACCACAGCACTCTGCCCCTCTGGCAGCCGCCGGGCCTCCCCTCCAGGGACGCC
CACACCAGAAGCAGATGCCACGCTACTAAAGAAGTCAGAGAAACTGTTGGCAGAGTTGGA
CCGGAGCGGGTTACCCTCTGCCCCTGGGGCCCCCAGACGAAGAGGCAGTATGCCTGTCCC
CTACAAGCACCAGCTCCGGCGGGCCCAGGCTGTAGATGAACTTGACTGGCCACCTCAGGC
CTCATCCTCTGGCTCGTCTGACTCCTTGGGCTCAGGGGAGGCAGCCCCTGCTCAAAAGGA
TGGCATCTTCAAGGTCATGCTAGTGGGGGAGAGCGGCGTGGGCAAGAGCACCCTAGCAGG
CACTTTTGGTGGTCTCCAGGGAGACAGTGCTCACGAACCGGAGAACCCAGAGGATACCTA
TGAGAGACGCATCATGGTGGATAAGGAGGAAGTGACTCTAGTCGTTTATGACATCTGGGA
ACAGGGGGATGCAGGAGGGTGGCTGCGGGACCACTGCCTTCAGACCGGGGACGCCTTTCT
CATCGTCTTCTCAGTCACCGACCGACGGAGTTTCTCCAAAGTTCCAGAGACCCTACTTCG
GCTCCGGGCTGGGAGGCCGCACCACGACCTACCCGTTATCCTCGTTGGAAACAAGAGCGA
CTTGGCCCGCTCCCGGGAGGTATCACTGGAGGAGGGCCGCCACCTGGCCGGGACGCTGAG
CTGCAAGCACATCGAGACGTCGGCCGCACTGCACCACAACACGAGGGAGCTCTTCGAGGG
CGCGGTGCGCCAGATCCGGCTGCGGCGGGGCCGAAACCACGCCGGAGGCCAGAGGCCCGA
TCCGGGCAGCCCCGAGGGCCCTGCGCCACCTGCACGCCGCGAGAGCCTCACCAAGAAAGC
CAAGAGGTTCCTCGCCAACCTGGTGCCGCGCAACGCCAAGTTCTTCAAGCAGCGCTCCAG
GTCGTGTCACGACCTCTCGGTGCTCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT