Restriction map for
pFN21AE4063
(Circular) (Six-base) MAPSORT of: pFN21AE4063.seq Check: 9337 from: 1 to: 5725
pFN21AE4063 5725 bp
With 182 enzymes: SgfI *
March 28, 2012 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2917 2917
Size: 5725
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2780 3535 278
Size: 53 83 186 936 1244 755 2468
Fragments arranged by size:
2468 1244 936 755 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3068 1937
Size: 1131 4594
Acc65I G'GTAC_C
Cuts at: 3046 3046
Size: 5725
AclI AA'CG_TT
Cuts at: 1563 3852 4225 1563
Size: 2289 373 3063
Fragments arranged by size:
3063 2289 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2543 3788 5172 5440 1632
Size: 911 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 911 268
AfeI AGC'GCT
Cuts at: 2993 2993
Size: 5725
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5528
AflIII A'CryG_T
Cuts at: 1203 4624 5601 1203
Size: 3421 977 1327
Fragments arranged by size:
3421 1327 977
AgeI A'CCGG_T
Cuts at: 4556 4556
Size: 5725
AhdI GACnn_n'nnGTC
Cuts at: 4454 4454
Size: 5725
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5725
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5725
AlwNI CAG_nnn'CTG
Cuts at: 2327 3468 5040 2327
Size: 1141 1572 3012
Fragments arranged by size:
3012 1572 1141
ApaI G_GGCC'C
Cuts at: 2191 2245 2900 2191
Size: 54 655 5016
Fragments arranged by size:
5016 655 54
ApaLI G'TGCA_C
Cuts at: 3782 4938 3782
Size: 1156 4569
ApoI r'AATT_y
Cuts at: 1493 3034 3260 3412 5521 1493
Size: 1541 226 152 2109 1697
Fragments arranged by size:
2109 1697 1541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4279 160
Size: 872 3247 1606
Fragments arranged by size:
3247 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
AvaI C'yCGr_G
Cuts at: 1870 1943 2712 2891 3050 1870
Size: 73 769 179 159 4545
Fragments arranged by size:
4545 769 179 159 73
BamHI G'GATC_C
Cuts at: 3055 3422 3055
Size: 367 5358
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 3046 4501 618
Size: 325 217 627 1175 84 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217 84
BanII G_rGCy'C
Cuts at: 1391 2191 2245 2312 2811 2900 3044 1391
Size: 800 54 67 499 89 144 4072
Fragments arranged by size:
4072 800 499 144 89 67 54
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2578 928
Size: 971 679 4075
Fragments arranged by size:
4075 971 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2752 2786 2882 2916 3916
Size: 34 50 34 731 34 96 34 1000
Cuts at: 3916 3950 1903
Size: 34 3678
Fragments arranged by size:
3678 1000 731 96 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2446 3617 4827 2446
Size: 1171 1210 3344
Fragments arranged by size:
3344 1210 1171
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5725
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 2186 2895
Size: 107 122 71 631 605 395 119 709
Cuts at: 2895 4336 136
Size: 1441 1525
Fragments arranged by size:
1525 1441 709 631 605 395 122 119
107 71
BglII A'GATC_T
Cuts at: 5720 5720
Size: 5725
BlpI GC'TnA_GC
Cuts at: 2756 3142 2756
Size: 386 5339
Bme1580I G_kGCm'C
Cuts at: 1790 2191 2245 2900 3786 4942 1790
Size: 401 54 655 886 1156 2573
Fragments arranged by size:
2573 1156 886 655 401 54
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5725
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3456 4414 442
Size: 416 53 83 399 24 2039 958 1753
Fragments arranged by size:
2039 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5725
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2073 2399 2747 2980 4385 1239
Size: 834 326 348 233 1405 2579
Fragments arranged by size:
2579 1405 834 348 326 233
Bpu10I CC'TnA_GC
Cuts at: 2311 2311
Size: 5725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4715 5013 3850
Size: 865 298 4562
Fragments arranged by size:
4562 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2417 2621 4388 882
Size: 617 918 204 1767 2219
Fragments arranged by size:
2219 1767 918 617 204
BsaAI yAC'GTr
Cuts at: 493 1204 3516 493
Size: 711 2312 2702
Fragments arranged by size:
2702 2312 711
BsaBI GATnn'nnATC
Cuts at: 5719 5719
Size: 5725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2096 2571 2777 3532
Size: 53 83 186 936 563 475 206 755
Cuts at: 3532 3914 4549 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 755 635 563 475 382 206
186 83 53
BsaWI w'CCGG_w
Cuts at: 2160 2437 4158 4556 4830 4977 5388 2160
Size: 277 1721 398 274 147 411 2497
Fragments arranged by size:
2497 1721 411 398 277 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2625 2655 1178
Size: 30 165 30 1222 30 4248
Fragments arranged by size:
4248 1222 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2744 1186
Size: 1558 4167
BseYI C'CCAG_C
Cuts at: 2648 4928 5424 2648
Size: 2280 496 2949
Fragments arranged by size:
2949 2280 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2774 2895 1163
Size: 573 1038 121 3993
Fragments arranged by size:
3993 1038 573 121
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2602 2784 3175 3936 4964 1540
Size: 275 186 601 182 391 761 1028 2301
Fragments arranged by size:
2301 1028 761 601 391 275 186 182
BsiHKAI G_wGCw'C
Cuts at: 2390 2431 2811 3024 3786 3871 4942 2390
Size: 41 380 213 762 85 1071 3173
Fragments arranged by size:
3173 1071 762 380 213 85 41
BsmI GAATG_Cn'
Cuts at: 3236 3329 3236
Size: 93 5632
BsmBI CGTCTCn'nnnn_
Cuts at: 2458 2768 2458
Size: 310 5415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2191 2245 2312 2390 2431 2811 2900
Size: 399 401 54 67 78 41 380 89
Cuts at: 2900 3024 3044 3786 3871 4942 1391
Size: 124 20 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 401 399 380 124 89
85 78 67 54 41 20
BspEI T'CCGG_A
Cuts at: 5388 5388
Size: 5725
BspHI T'CATG_A
Cuts at: 3614 5344 3614
Size: 1730 3995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2917 3080 844
Size: 2073 163 3489
Fragments arranged by size:
3489 2073 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2166 2373 2709 3612 1394
Size: 429 343 207 336 903 3507
Fragments arranged by size:
3507 903 429 343 336 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 4220 4394 65
Size: 1183 2972 174 1396
Fragments arranged by size:
2972 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4369 4556 1700
Size: 110 2559 187 2869
Fragments arranged by size:
2869 2559 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5725
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5725
BssSI C'ACGA_G
Cuts at: 2800 3785 4797 2800
Size: 985 1012 3728
Fragments arranged by size:
3728 1012 985
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2327 794
Size: 1533 4192
BstBI TT'CG_AA
Cuts at: 4529 4529
Size: 5725
BstEII G'GTnAC_C
Cuts at: 1184 2169 1184
Size: 985 4740
BstYI r'GATC_y
Cuts at: 1972 2832 3055 3422 3807 3824 5276 5362 5374
Size: 860 223 367 385 17 1452 86 12
Cuts at: 5374 5720 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 860 385 367 346 223 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2274 2274
Size: 5725
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4986
Fragments arranged by size:
4986 683 56
BtsI GCAGTG_nn'
Cuts at: 2551 2786 3241 4035 4055 2551
Size: 235 455 794 20 4221
Fragments arranged by size:
4221 794 455 235 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5725
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5690
DraI TTT'AAA
Cuts at: 3029 3029
Size: 5725
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4732 809
Size: 507 3416 1802
Fragments arranged by size:
3416 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2267 2745 2781
Size: 54 1425 50 275 18 437 478 36
Cuts at: 2781 3172 4061 5690 8
Size: 391 889 1629 43
Fragments arranged by size:
1629 1425 889 478 437 391 275 54
50 43 36 18
EagI C'GGCC_G
Cuts at: 1537 1812 2781 3172 1537
Size: 275 969 391 4090
Fragments arranged by size:
4090 969 391 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2194 2816 3655 1353
Size: 841 622 839 3423
Fragments arranged by size:
3423 841 839 622
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4312 4686 4832 387
Size: 1064 138 2723 374 146 1280
Fragments arranged by size:
2723 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2809 2809
Size: 5725
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2073 2399 2543 2747 2980 3788 4385
Size: 393 441 326 144 204 233 808 597
Cuts at: 4385 5172 5440 1239
Size: 787 268 1524
Fragments arranged by size:
1524 808 787 597 441 393 326 268
233 204 144
EcoO109I rG'GnC_Cy
Cuts at: 2187 2188 2896 2897 2187
Size: 1 708 1 5015
Fragments arranged by size:
5015 708 1 1
EcoRI G'AATT_C
Cuts at: 3034 3412 3034
Size: 378 5347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 5725
FspI TGC'GCA
Cuts at: 4231 4231
Size: 5725
HaeII r_GCGC'y
Cuts at: 1678 2995 4872 1678
Size: 1317 1877 2531
Fragments arranged by size:
2531 1877 1317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2863 2895 4366 4398 4440
Size: 32 135 32 1291 32 1471 32 42
Cuts at: 4440 4472 1373
Size: 32 2626
Fragments arranged by size:
2626 1471 1291 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3069 3315 669
Size: 988 281 1131 246 3079
Fragments arranged by size:
3079 1131 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5725
HpaI GTT'AAC
Cuts at: 3315 3315
Size: 5725
KpnI G_GTAC'C
Cuts at: 3050 3050
Size: 5725
MfeI C'AATT_G
Cuts at: 3324 3324
Size: 5725
MluI A'CGCG_T
Cuts at: 5601 5601
Size: 5725
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2136 2665 4839 5023 1305
Size: 831 529 2174 184 2007
Fragments arranged by size:
2174 2007 831 529 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2269 10
Size: 54 1425 343 437 3466
Fragments arranged by size:
3466 1425 437 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2027 2033 2360 2474 3683 4042 4201
Size: 648 861 6 327 114 1209 359 159
Cuts at: 4201 518
Size: 2042
Fragments arranged by size:
2042 1209 861 648 359 327 159 114
6
MspA1I CmG'CkG
Cuts at: 1606 3141 3818 4966 5211 1606
Size: 1535 677 1148 245 2120
Fragments arranged by size:
2120 1535 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5173
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5725
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5725
NotI GC'GGCC_GC
Cuts at: 3172 3172
Size: 5725
NspI r_CATG'y
Cuts at: 1132 1229 3083 4628 1132
Size: 97 1854 1545 2229
Fragments arranged by size:
2229 1854 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2183 1348
Size: 835 4890
PciI A'CATG_T
Cuts at: 4624 4624
Size: 5725
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5725
PmeI GTTT'AAAC
Cuts at: 3029 3029
Size: 5725
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 5307 5339 3794
Size: 32 1481 32 4180
Fragments arranged by size:
4180 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2498 1613
Size: 885 4840
PsiI TTA'TAA
Cuts at: 3295 3295
Size: 5725
PspOMI G'GGCC_C
Cuts at: 2187 2241 2896 2187
Size: 54 655 5016
Fragments arranged by size:
5016 655 54
PstI C_TGCA'G
Cuts at: 830 3077 830
Size: 2247 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5725
SacI G_AGCT'C
Cuts at: 2811 2811
Size: 5725
SalI G'TCGA_C
Cuts at: 1936 3067 1936
Size: 1131 4594
SapI GCTCTTCn'nnn_
Cuts at: 2816 2816
Size: 5725
SbfI CC_TGCA'GG
Cuts at: 3077 3077
Size: 5725
ScaI AGT'ACT
Cuts at: 3973 5685 3973
Size: 1712 4013
SexAI A'CCwGG_T
Cuts at: 2958 2958
Size: 5725
SfcI C'TryA_G
Cuts at: 826 1045 2250 3073 4208 4889 5080 826
Size: 219 1205 823 1135 681 191 1471
Fragments arranged by size:
1471 1205 1135 823 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5725
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5725
SmaI CCC'GGG
Cuts at: 1872 2714 3052 1872
Size: 842 338 4545
Fragments arranged by size:
4545 842 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3829 4730 4992 820
Size: 197 926 1886 901 262 1553
Fragments arranged by size:
1886 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3516 493
Size: 3023 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5725
SphI G_CATG'C
Cuts at: 1132 3083 1132
Size: 1951 3774
SspI AAT'ATT
Cuts at: 5 52 3649 5
Size: 47 3597 2081
Fragments arranged by size:
3597 2081 47
StuI AGG'CCT
Cuts at: 1517 2279 1517
Size: 762 4963
StyI C'CwwG_G
Cuts at: 513 1065 2304 3164 513
Size: 552 1239 860 3074
Fragments arranged by size:
3074 1239 860 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2616 3752 3769 3922 4107 4552 325
Size: 1255 1036 1136 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1136 1036 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3971 5683 96
Size: 274 80 33 51 1443 1994 1712 138
Fragments arranged by size:
1994 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5685 5717 5685
Size: 32 5693
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5725
XbaI T'CTAG_A
Cuts at: 3061 3061
Size: 5725
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5725
XmaI C'CCGG_G
Cuts at: 1870 2712 3050 1870
Size: 842 338 4545
Fragments arranged by size:
4545 842 338
XmnI GAAnn'nnTTC
Cuts at: 3854 3854
Size: 5725
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2778 3533 276
Size: 53 83 186 936 1244 755 2468
Fragments arranged by size:
2468 1244 936 755 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI
SfiI SfoI SrfI SwaI XcmI