################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BclI BfrI BglII BlnI BsaAI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AatII 771 GACGTC GACGT^C AlwNI 317 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 268 CCTCAGG CC^T[ACGT]AGG Aor51HI 986 AGCGCT AGC^GCT BalI 262 TGGCCA TGG^CCA BanI 957 GGTGCC G^G[CT][GA]CC BbsI 580 GTCTTC GAAGAC(2/6) BciVI 447 GGATAC GTATCC(6/5) BlpI 750 GCTGAGC GC^T[ACGT]AGC Bpu1102I 750 GCTGAGC GC^T[ACGT]AGC BpuAI 580 GTCTTC GAAGAC(2/6) BseRI 724 GAGGAG GAGGAG(10/8) BspMI 903 ACCTGC ACCTGC(4/8) BssSI 795 CACGAG CACGAG(-5/-1) BstAPI 316 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 164 GGTTACC G^GT[ACGT]ACC BstPI 164 GGTTACC G^GT[ACGT]ACC BstYI 827 AGATCC [GA]^GATC[CT] Bsu36I 268 CCTCAGG CC^T[ACGT]AGG CelII 750 GCTGAGC GC^T[ACGT]AGC EagI 776 CGGCCG C^GGCCG EclXI 776 CGGCCG C^GGCCG Eco47III 986 AGCGCT AGC^GCT Eco52I 776 CGGCCG C^GGCCG Eco81I 268 CCTCAGG CC^T[ACGT]AGG EcoO65I 164 GGTTACC G^GT[ACGT]ACC EcoT14I 299 CCTTGG C^C[AT][AT]GG HaeII 986 AGCGCT [GA]GCGC^[CT] MaeII 772 ACGT A^CGT MflI 827 AGATCC [GA]^GATC[CT] MluNI 262 TGGCCA TGG^CCA MscI 262 TGGCCA TGG^CCA PshAI 489 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 802 GAGCTC GAGCT^C SapI 804 GCTCTTC GCTCTTC(1/4) SexAI 953 ACCTGGT A^CC[AT]GGT SfcI 245 CTGTAG C^T[GA][CT]AG SmaI 707 CCCGGG CCC^GGG StuI 272 AGGCCT AGG^CCT StyI 299 CCTTGG C^C[AT][AT]GG XhoII 827 AGATCC [GA]^GATC[CT] XmaCI 707 CCCGGG C^CCGGG XmaI 707 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwI 828 GATCC GGATC(4/5) 874 GATCC AvaI 707 CCCGGG C^[CT]CG[GA]G 886 CCCGAG BcgI 888 CGAGGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 758 GCAAGCACATCG BsaI 622 GAGACC GGTCTC(1/5) 406 GGTCTC BsaWI 155 ACCGGA [AT]^CCGG[AT] 432 ACCGGA BsgI 784 CTGCAC GTGCAG(16/14) 905 CTGCAC BsiEI 594 CGACCG CG[GA][CT]^CG 776 CGGCCG BsmBI 459 GAGACG CGTCTC(1/5) 769 GAGACG BsoBI 707 CCCGGG C^[CT]CG[GA]G 886 CCCGAG BsrI 260 ACTGG ACTGG(1/-1) 720 ACTGG DpnII 828 GATC ^GATC 874 GATC EarI 805 CTCTTC CTCTTC(1/4) 194 GAAGAG HinfI 295 GACTC G^A[ACGT]TC 489 GACTC HphI 590 TCACC GGTGA(8/7) 923 TCACC Ksp632I 805 CTCTTC CTCTTC(1/4) 194 GAAGAG MboI 828 GATC ^GATC 874 GATC NdeII 828 GATC ^GATC 874 GATC PleI 295 GACTC GAGTC(4/5) 489 GACTC Sau3AI 828 GATC ^GATC 874 GATC TaqI 767 TCGA T^CGA 809 TCGA TthHB8I 767 TCGA T^CGA 809 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AspHI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeI MaeIII MboII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI Tsp45I TspRI