SPSB2 ORF clone pFN21AE4044

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4044

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Clone info.

Species

human

Clone Name

pFN21AE4044 Sequence (with vector) Restriction Map

Product ID

FHC11147

ORF Sequence

DNA sequence (801 bp)
Protein sequence (267 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4044

Symbol

SPSB2

Alias : GRCC9, SSB2

Description

splA/ryanodine receptor domain and SOCS box containing 2

Original Clone ID

cp94044

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 801 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 267 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8567.1 1.7e-93 88.8 SPSB2 CCDS3115.1 3.4e-44 59.0 SPSB4 CCDS102.1 2.5e-42 51.5 SPSB1 CCDS46985.1 1.4e-28 46.3 FBXO45 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003877 87 218 PF00622 SPla/RYanodine receptor SPRY HMMSmart IPR018355 86 220 SM00449 SPla/RYanodine receptor subgroup ProfileScan IPR001870 26 221 PS50188 B302/SPRY domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001139788 263 611658 88.8 97.4 NP_001306599 263 611658 88.8 97.4 NP_116030 263 611658 88.8 97.4 NP_079382 273 611657 51.5 81.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4044 Download

>KIEE4044  801 bp
ATGGGCCAGACAGCTCTGGCAGGGGGCAGCAGCAGCACCCCCACGCCACAGGCCCTGTAC
CCTGACCTCTCCTGTCCCGAGGGCTTGGAAGAGCTGCTGTCTGCACCCCCTCCTGACCTG
GGGGCCCAGCGGCGCCACGGTTGGAACCCCAAAGACTGTTCAGAGAACATCGAGGTCAAG
GAAGGAGGGTTGTACTTTGAGCGGCGGCCCGTGGCCCAGAGCACTGATGGGGCCCGGGGT
AAGAGGGGCTATTCAAGGGGCCTGCACGCCTGGGAGATCAGCTGGCCCCTAGAGCAGAGG
GGCACGCATGCCGTGGTGGGCGTGGCCACGGCCCTCGCCCCGCTGCAGACTGACCACTAC
GCGGCGCTGCTGGGCAGCAACAGCGAGTCGTGGGGCTGGGACATCGGGCGGGGGAAGCTG
TACCATCAGAGCAAGGGGCCCGGAGCCCCCCAGTATCCAGCGGGAACTCAGGGTGAGCAG
CTGGAGGTGCCAGAGAGACTGCTGGTGGTTCTGGACATGGAGGAGGGAACTCTGGGCTAC
GCTATTGGGGGCACCTACCTGGGGCCAGCATTCCGCGGACTGAAGGGCAGGACCCTCTAT
CCGGCAGTAAGCGCTGTCTGGGGCCAGTGCCAGGTCCGCATCCGCTACCTGGGCGAAAGG
AGAGGTGAGGCCTGGGGCAGACGTGGGGAGAACTTTCTGTCCCTGGTGGCAGTGGTTTGG
GATGGAAACTCTTCTGACAAGAGCAGAGGGGATGGACCTTCATCCAGCCTGCCTCAACCT
CTGTTCAGTGCTGGGAAAGGC

ORF Protein

Cloned ORF protein sequence for pFN21AE4044 Download

>KIEE4044  267 aa
MGQTALAGGSSSTPTPQALYPDLSCPEGLEELLSAPPPDLGAQRRHGWNPKDCSENIEVK
EGGLYFERRPVAQSTDGARGKRGYSRGLHAWEISWPLEQRGTHAVVGVATALAPLQTDHY
AALLGSNSESWGWDIGRGKLYHQSKGPGAPQYPAGTQGEQLEVPERLLVVLDMEEGTLGY
AIGGTYLGPAFRGLKGRTLYPAVSAVWGQCQVRIRYLGERRGEAWGRRGENFLSLVAVVW
DGNSSDKSRGDGPSSSLPQPLFSAGKG

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4044 Download

>pFN21AE4044  5506 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCCAGACAGCTCTGGCAGGGGGCAGCAGCAG
CACCCCCACGCCACAGGCCCTGTACCCTGACCTCTCCTGTCCCGAGGGCTTGGAAGAGCT
GCTGTCTGCACCCCCTCCTGACCTGGGGGCCCAGCGGCGCCACGGTTGGAACCCCAAAGA
CTGTTCAGAGAACATCGAGGTCAAGGAAGGAGGGTTGTACTTTGAGCGGCGGCCCGTGGC
CCAGAGCACTGATGGGGCCCGGGGTAAGAGGGGCTATTCAAGGGGCCTGCACGCCTGGGA
GATCAGCTGGCCCCTAGAGCAGAGGGGCACGCATGCCGTGGTGGGCGTGGCCACGGCCCT
CGCCCCGCTGCAGACTGACCACTACGCGGCGCTGCTGGGCAGCAACAGCGAGTCGTGGGG
CTGGGACATCGGGCGGGGGAAGCTGTACCATCAGAGCAAGGGGCCCGGAGCCCCCCAGTA
TCCAGCGGGAACTCAGGGTGAGCAGCTGGAGGTGCCAGAGAGACTGCTGGTGGTTCTGGA
CATGGAGGAGGGAACTCTGGGCTACGCTATTGGGGGCACCTACCTGGGGCCAGCATTCCG
CGGACTGAAGGGCAGGACCCTCTATCCGGCAGTAAGCGCTGTCTGGGGCCAGTGCCAGGT
CCGCATCCGCTACCTGGGCGAAAGGAGAGGTGAGGCCTGGGGCAGACGTGGGGAGAACTT
TCTGTCCCTGGTGGCAGTGGTTTGGGATGGAAACTCTTCTGACAAGAGCAGAGGGGATGG
ACCTTCATCCAGCCTGCCTCAACCTCTGTTCAGTGCTGGGAAAGGCGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT