(Linear) (Six-base) MAPSORT of: KIEE4044.seq Check: 821 from: 1 to: 801 KIEE4044.orf With 182 enzymes: SgfI * March 22, 2012 17:14 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 801 Size: 601 200 AfeI AGC'GCT Cuts at: 0 612 801 Size: 612 189 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 682 714 801 Size: 682 32 87 Fragments arranged by size: 682 87 32 AlwNI CAG_nnn'CTG Cuts at: 0 54 801 Size: 54 747 ApaI G_GGCC'C Cuts at: 0 126 234 440 801 Size: 126 108 206 361 Fragments arranged by size: 361 206 126 108 AvaI C'yCGr_G Cuts at: 0 76 233 801 Size: 76 157 568 Fragments arranged by size: 568 157 76 BanI G'GyrC_C Cuts at: 0 131 486 550 801 Size: 131 355 64 251 Fragments arranged by size: 355 251 131 64 BanII G_rGCy'C Cuts at: 0 126 234 440 447 801 Size: 126 108 206 7 354 Fragments arranged by size: 354 206 126 108 7 BbeI G_GCGC'C Cuts at: 0 135 801 Size: 135 666 BciVI GTATCCnnnnn_n' Cuts at: 0 464 801 Size: 464 337 Bme1580I G_kGCm'C Cuts at: 0 126 234 304 440 553 801 Size: 126 108 70 136 113 248 Fragments arranged by size: 248 136 126 113 108 70 BmgBI CAC'GTC Cuts at: 0 682 801 Size: 682 119 BmrI ACTGGGnnnn_n' Cuts at: 0 444 801 Size: 444 357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 801 Size: 502 299 BsaHI Gr'CG_yC Cuts at: 0 132 801 Size: 132 669 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 535 801 Size: 535 266 BseYI C'CCAG_C Cuts at: 0 125 369 395 790 801 Size: 125 244 26 395 11 Fragments arranged by size: 395 244 125 26 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 86 247 801 Size: 86 161 554 Fragments arranged by size: 554 161 86 BsiHKAI G_wGCw'C Cuts at: 0 223 801 Size: 223 578 BsmI GAATG_Cn' Cuts at: 0 568 801 Size: 568 233 Bsp1286I G_dGCh'C Cuts at: 0 126 223 234 304 440 447 553 801 Size: 126 97 11 70 136 7 106 248 Fragments arranged by size: 248 136 126 106 97 70 11 7 BsrBI CCG'CTC Cuts at: 0 201 801 Size: 201 600 BtgI C'CryG_G Cuts at: 0 135 209 311 326 573 801 Size: 135 74 102 15 247 228 Fragments arranged by size: 247 228 135 102 74 15 BtsI GCAGTG_nn' Cuts at: 0 716 801 Size: 716 85 EaeI y'GGCC_r Cuts at: 0 323 801 Size: 323 478 EarI CTCTTCn'nnn_ Cuts at: 0 83 735 801 Size: 83 652 66 Fragments arranged by size: 652 83 66 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 502 601 801 Size: 502 99 200 Fragments arranged by size: 502 200 99 EcoO109I rG'GnC_Cy Cuts at: 0 51 122 230 258 436 590 801 Size: 51 71 108 28 178 154 211 Fragments arranged by size: 211 178 154 108 71 51 28 HaeII r_GCGC'y Cuts at: 0 135 367 614 801 Size: 135 232 247 187 Fragments arranged by size: 247 232 187 135 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 82 114 582 614 712 744 801 Size: 82 32 468 32 98 32 57 Fragments arranged by size: 468 98 82 57 32 32 32 KasI G'GCGC_C Cuts at: 0 131 801 Size: 131 670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 121 801 Size: 121 680 MscI TGG'CCA Cuts at: 0 325 801 Size: 325 476 MspA1I CmG'CkG Cuts at: 0 129 281 342 460 480 575 801 Size: 129 152 61 118 20 95 226 Fragments arranged by size: 226 152 129 118 95 61 20 NarI GG'CG_CC Cuts at: 0 132 801 Size: 132 669 NspI r_CATG'y Cuts at: 0 310 801 Size: 310 491 PpuMI rG'GwC_Cy Cuts at: 0 590 801 Size: 590 211 PspOMI G'GGCC_C Cuts at: 0 122 230 436 801 Size: 122 108 206 365 Fragments arranged by size: 365 206 122 108 PstI C_TGCA'G Cuts at: 0 347 801 Size: 347 454 PvuII CAG'CTG Cuts at: 0 281 480 801 Size: 281 199 321 Fragments arranged by size: 321 281 199 SacII CC_GC'GG Cuts at: 0 576 801 Size: 576 225 SapI GCTCTTCn'nnn_ Cuts at: 0 83 801 Size: 83 718 SfcI C'TryA_G Cuts at: 0 343 801 Size: 343 458 SfoI GGC'GCC Cuts at: 0 133 801 Size: 133 668 SmaI CCC'GGG Cuts at: 0 235 801 Size: 235 566 SphI G_CATG'C Cuts at: 0 310 801 Size: 310 491 StuI AGG'CCT Cuts at: 0 670 801 Size: 670 131 TatI w'GTAC_w Cuts at: 0 191 801 Size: 191 610 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 223 801 Size: 223 578 XmaI C'CCGG_G Cuts at: 0 233 801 Size: 233 568 XmnI GAAnn'nnTTC Cuts at: 0 729 801 Size: 729 72 Enzymes that do cut: AcuI AfeI AloI AlwNI ApaI AvaI BanI BanII BbeI BciVI Bme1580I BmgBI BmrI BpmI BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BsrBI BtgI BtsI EaeI EarI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MspA1I NarI NspI PpuMI PspOMI PstI PvuII SacII SapI SfcI SfoI SmaI SphI StuI TatI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StyI SwaI TaqII TstI Tth111I XbaI XhoI ZraI