################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI MaeIII MamI MfeI MflI MluI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcyI 131 GGCGCC G[GA]^CG[CT]C AlwNI 49 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 610 AGCGCT AGC^GCT Asp700I 725 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 219 GAGCAC G[AT]GC[AT]^C BalI 323 TGGCCA TGG^CCA BbeI 131 GGCGCC GGCGC^C BciVI 453 GTATCC GTATCC(6/5) BfaI 289 CTAG C^TAG BpmI 481 CTGGAG CTGGAG(16/14) BsaHI 131 GGCGCC G[GA]^CG[CT]C BseRI 520 GAGGAG GAGGAG(10/8) BsiHKAI 219 GAGCAC G[AT]GC[AT]^C BsmAI 495 GAGAC GTCTC(1/5) BsmI 568 GCATTC GAATGC(1/-1) BsrBI 199 GAGCGG CCGCTC(-3/-3) DdeI 467 CTCAG C^T[ACGT]AG DpnII 276 GATC ^GATC EaeI 323 TGGCCA [CT]^GGCC[GA] Eco47III 610 AGCGCT AGC^GCT Hin1I 131 GGCGCC G[GA]^CG[CT]C HinfI 385 GAGTC G^A[ACGT]TC KasI 131 GGCGCC G^GCGCC KspI 573 CCGCGG CCGC^GG MaeI 289 CTAG C^TAG MaeII 681 ACGT A^CGT MboI 276 GATC ^GATC MluNI 323 TGGCCA TGG^CCA MscI 323 TGGCCA TGG^CCA NarI 131 GGCGCC GG^CGCC NdeII 276 GATC ^GATC NspI 306 GCATGC [GA]CATG^[CT] PleI 385 GAGTC GAGTC(4/5) PpuMI 589 AGGACCC [GA]G^G[AT]CC[CT] PstI 343 CTGCAG CTGCA^G SacII 573 CCGCGG CCGC^GG SapI 88 GAAGAGC GCTCTTC(1/4) Sau3AI 276 GATC ^GATC SfaNI 638 GCATC GCATC(5/9) SfcI 343 CTGCAG C^T[GA][CT]AG SfoI 131 GGCGCC GGC^GCC SmaI 233 CCCGGG CCC^GGG SphI 306 GCATGC GCATG^C StuI 668 AGGCCT AGG^CCT TaqI 170 TCGA T^CGA TthHB8I 170 TCGA T^CGA XcmI 216 CCAGAGCACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 233 CCCGGG C^CCGGG XmaI 233 CCCGGG C^CCGGG XmnI 725 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AccII 360 CGCG CG^CG 574 CGCG AvaI 76 CCCGAG C^[CT]CG[GA]G 233 CCCGGG BsgI 101 CTGCAC GTGCAG(16/14) 262 CTGCAC BsoBI 76 CCCGAG C^[CT]CG[GA]G 233 CCCGGG BsrI 450 CCAGT ACTGG(1/-1) 624 CCAGT BstUI 360 CGCG CG^CG 574 CGCG EarI 729 CTCTTC CTCTTC(1/4) 88 GAAGAG HphI 472 GGTGA GGTGA(8/7) 664 GGTGA Ksp632I 729 CTCTTC CTCTTC(1/4) 88 GAAGAG MboII 88 GAAGA GAAGA(8/7) 730 TCTTC MvnI 360 CGCG CG^CG 574 CGCG NlaIII 307 CATG CATG^ 516 CATG PvuII 279 CAGCTG CAG^CTG 478 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI ApaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI Cac8I CfoI Cfr13I DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIV RsaI Sau96I ScrFI TseI TspRI