MARCH3 ORF clone pFN21AE4038

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE4038

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Clone info.

Species

human

Clone Name

pFN21AE4038 Sequence (with vector) Restriction Map

Product ID

FHC22269

ORF Sequence

DNA sequence (759 bp)
Protein sequence (253 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE4038

Symbol

MARCH3

Description

membrane-associated ring finger (C3HC4) 3, E3 ubiquitin protein ligase

Original Clone ID

cp94038

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 759 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 253 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS4141.1 2.9e-110 100.0 MARCH3 CCDS12202.1 6.2e-66 64.7 MARCH2 CCDS32894.1 1.2e-30 63.4 MARCH2 CCDS3806.1 1.1e-13 30.2 MARCH1 CCDS54814.1 1.1e-13 30.2 MARCH1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR018957 71 116 PF00097 Zinc finger HMMSmart IPR011016 70 117 SM00744 Zinc finger ProfileScan IPR011016 63 123 PS51292 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 146 FGDMVCFLFITPLATISGWLCLR 168 PRIMARY 23 2 182 AVGLIALTVALFTIYLFWTLVSF 204 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_848545 253 613333 100.0 100.0 NP_057580 246 613332 64.7 100.0 NP_001005415 246 613332 64.7 100.0 XP_016882342 246 613332 64.7 100.0 XP_011526348 256 613332 62.3 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE4038 Download

>KIEE4038  759 bp
ATGACAACCAGCCGCTGCAGTCACCTGCCCGAAGTCCTGCCAGACTGCACCAGCTCAGCT
GCACCCGTGGTGAAGACGGTGGAGGATTGTGGCAGCCTAGTGAATGGGCAGCCGCAGTAT
GTCATGCAAGTTTCAGCCAAGGACGGGCAGCTGCTGTCAACAGTAGTGCGGACTCTTGCC
ACCCAGAGCCCCTTCAATGACCGGCCGATGTGCAGGATCTGCCACGAGGGCAGCAGCCAA
GAGGACTTGCTCTCTCCATGTGAATGTACAGGGACCTTGGGGACAATTCATCGGAGCTGC
CTGGAGCACTGGCTGTCATCCTCAAACACCAGCTACTGTGAACTCTGCCACTTCAGGTTT
GCAGTCGAGCGCAAACCCAGGCCGTTAGTGGAGTGGCTGAGAAACCCTGGCCCCCAGCAT
GAGAAGCGGACTCTGTTTGGCGACATGGTGTGCTTCTTGTTTATAACTCCCCTGGCCACC
ATCTCGGGCTGGCTGTGCCTGCGGGGCGCCGTGGACCACCTGCACTTTAGTAGTCGGCTG
GAAGCCGTCGGACTGATTGCACTCACTGTCGCACTCTTCACTATTTACCTCTTTTGGACA
CTAGTGTCATTTAGGTACCACTGTCGATTGTACAACGAGTGGCGTCGGACCAATCAGAGG
GTGATTCTCCTCATTCCAAAGTCTGTCAATGTACCTTCTAACCAGCCGTCCTTGCTGGGC
CTCCATTCGGTCAAGAGGAACTCAAAGGAGACAGTTGTT

ORF Protein

Cloned ORF protein sequence for pFN21AE4038 Download

>KIEE4038  253 aa
MTTSRCSHLPEVLPDCTSSAAPVVKTVEDCGSLVNGQPQYVMQVSAKDGQLLSTVVRTLA
TQSPFNDRPMCRICHEGSSQEDLLSPCECTGTLGTIHRSCLEHWLSSSNTSYCELCHFRF
AVERKPRPLVEWLRNPGPQHEKRTLFGDMVCFLFITPLATISGWLCLRGAVDHLHFSSRL
EAVGLIALTVALFTIYLFWTLVSFRYHCRLYNEWRRTNQRVILLIPKSVNVPSNQPSLLG
LHSVKRNSKETVV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE4038 Download

>pFN21AE4038  5464 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGACAACCAGCCGCTGCAGTCACCTGCCCGAAGT
CCTGCCAGACTGCACCAGCTCAGCTGCACCCGTGGTGAAGACGGTGGAGGATTGTGGCAG
CCTAGTGAATGGGCAGCCGCAGTATGTCATGCAAGTTTCAGCCAAGGACGGGCAGCTGCT
GTCAACAGTAGTGCGGACTCTTGCCACCCAGAGCCCCTTCAATGACCGGCCGATGTGCAG
GATCTGCCACGAGGGCAGCAGCCAAGAGGACTTGCTCTCTCCATGTGAATGTACAGGGAC
CTTGGGGACAATTCATCGGAGCTGCCTGGAGCACTGGCTGTCATCCTCAAACACCAGCTA
CTGTGAACTCTGCCACTTCAGGTTTGCAGTCGAGCGCAAACCCAGGCCGTTAGTGGAGTG
GCTGAGAAACCCTGGCCCCCAGCATGAGAAGCGGACTCTGTTTGGCGACATGGTGTGCTT
CTTGTTTATAACTCCCCTGGCCACCATCTCGGGCTGGCTGTGCCTGCGGGGCGCCGTGGA
CCACCTGCACTTTAGTAGTCGGCTGGAAGCCGTCGGACTGATTGCACTCACTGTCGCACT
CTTCACTATTTACCTCTTTTGGACACTAGTGTCATTTAGGTACCACTGTCGATTGTACAA
CGAGTGGCGTCGGACCAATCAGAGGGTGATTCTCCTCATTCCAAAGTCTGTCAATGTACC
TTCTAACCAGCCGTCCTTGCTGGGCCTCCATTCGGTCAAGAGGAACTCAAAGGAGACAGT
TGTTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCA
TGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCG
CTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATAC
ATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAA
ATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAAC
AACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGC
AAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCG
TTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGC
GGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTG
CGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGA
CAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT
TTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCA
GAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATC
GAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCA
ATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGG
CAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCA
GTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATA
ACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAG
CTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCG
GAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCA
ACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTA
ATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCT
GGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCA
GCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAG
GCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAT
TGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGG
TAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCC
AGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCC
CCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGAC
TATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCC
TGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATA
GCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGC
ACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCA
ACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAG
CGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTA
GAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTG
GTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGC
AGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGT
CTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAA
GGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCT
TCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGT
GGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGC
GGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAA
CGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTC
GTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAG
ATCT