(Circular) (Six-base) MAPSORT of: pFN21AE4038.seq Check: 9887 from: 1 to: 5464 pFN21AE4038 5464 bp With 182 enzymes: SgfI * March 28, 2012 13:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2037 2532 2037 Size: 495 4969 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2619 2785 2619 Size: 166 5298 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2341 3527 4911 5179 1632 Size: 709 1186 1384 268 1917 Fragments arranged by size: 1917 1384 1186 709 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2045 1184 Size: 861 4603 AlwNI CAG_nnn'CTG Cuts at: 2019 2158 2223 2340 3207 4779 2019 Size: 139 65 117 867 1572 2704 Fragments arranged by size: 2704 1572 867 139 117 65 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2488 2789 1870 Size: 73 545 301 4545 Fragments arranged by size: 4545 545 301 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2611 2644 2611 Size: 33 5431 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2510 2619 2785 4240 618 Size: 325 217 627 723 109 166 1455 1842 Fragments arranged by size: 1842 1455 723 627 325 217 166 109 BanII G_rGCy'C Cuts at: 1391 2195 2783 1391 Size: 804 588 4072 Fragments arranged by size: 4072 804 588 BbeI G_GCGC'C Cuts at: 2514 2514 Size: 5464 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2084 928 Size: 971 185 4308 Fragments arranged by size: 4308 971 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2232 2508 4075 Size: 107 122 71 631 605 560 276 1567 Cuts at: 4075 136 Size: 1525 Fragments arranged by size: 1567 1525 631 605 560 276 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2059 2881 2059 Size: 822 4642 Bme1580I G_kGCm'C Cuts at: 1790 3525 4681 1790 Size: 1735 1156 2573 Fragments arranged by size: 2573 1735 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3195 4153 442 Size: 416 53 83 399 24 1778 958 1753 Fragments arranged by size: 1778 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 4124 1239 Size: 1088 1797 2579 Fragments arranged by size: 2579 1797 1088 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4127 882 Size: 617 2628 2219 Fragments arranged by size: 2628 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2511 2647 3271 3653 Size: 53 83 186 936 978 136 624 382 Cuts at: 3653 4288 275 Size: 635 1451 Fragments arranged by size: 1451 978 936 635 624 382 186 136 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2663 1186 Size: 1477 3987 BseYI C'CCAG_C Cuts at: 2418 2719 4667 5163 2418 Size: 301 1948 496 2719 Fragments arranged by size: 2719 1948 496 301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2035 2049 2236 2510 1163 Size: 573 299 14 187 274 4117 Fragments arranged by size: 4117 573 299 274 187 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2210 2914 3675 4703 1540 Size: 275 186 209 704 761 1028 2301 Fragments arranged by size: 2301 1028 761 704 275 209 186 BsiHKAI G_wGCw'C Cuts at: 2313 3525 3610 4681 2313 Size: 1212 85 1071 3096 Fragments arranged by size: 3096 1212 1071 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2195 2313 2783 3525 3610 4681 1391 Size: 399 405 118 470 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 470 405 399 118 85 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2037 2532 2819 844 Size: 1193 495 287 3489 Fragments arranged by size: 3489 1193 495 287 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3959 4133 65 Size: 1183 2711 174 1396 Fragments arranged by size: 2711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2205 4108 4295 1700 Size: 110 395 1903 187 2869 Fragments arranged by size: 2869 1903 395 187 110 BsrGI T'GTAC_A Cuts at: 96 2270 2634 96 Size: 2174 364 2926 Fragments arranged by size: 2926 2174 364 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 2228 3524 4536 2228 Size: 1296 1012 3156 Fragments arranged by size: 3156 1296 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2223 794 Size: 1429 4035 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2220 2794 3161 3546 3563 5015 5101 5113 Size: 248 574 367 385 17 1452 86 12 Cuts at: 5113 5459 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 574 385 367 346 248 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2070 2514 513 Size: 552 1005 444 3463 Fragments arranged by size: 3463 1005 552 444 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2980 3774 3794 2980 Size: 794 20 4650 Fragments arranged by size: 4650 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2608 4471 809 Size: 507 1292 1863 1802 Fragments arranged by size: 1863 1802 1292 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2207 2478 2911 Size: 54 1425 50 275 18 377 271 433 Cuts at: 2911 3800 5429 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 433 377 275 271 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2207 2911 1537 Size: 275 395 704 4090 Fragments arranged by size: 4090 704 395 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2585 3394 1353 Size: 1232 809 3423 Fragments arranged by size: 3423 1232 809 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2341 3527 4124 4911 5179 1239 Size: 393 695 14 1186 597 787 268 1524 Fragments arranged by size: 1524 1186 787 695 597 393 268 14 EcoO109I rG'GnC_Cy Cuts at: 2277 2277 Size: 5464 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 2514 4611 1678 Size: 836 2097 2531 Fragments arranged by size: 2531 2097 836 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 2278 2310 4105 Size: 32 135 32 668 32 6 32 1795 Cuts at: 4105 4137 4179 4211 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1795 668 135 42 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 2163 2808 3054 669 Size: 988 281 225 645 246 3079 Fragments arranged by size: 3079 988 645 281 246 225 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KasI G'GCGC_C Cuts at: 2510 2510 Size: 5464 KpnI G_GTAC'C Cuts at: 2623 2789 2623 Size: 166 5298 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2533 2630 4578 4762 1305 Size: 1228 97 1948 184 2007 Fragments arranged by size: 2007 1948 1228 184 97 MscI TGG'CCA Cuts at: 10 64 1489 1832 2480 10 Size: 54 1425 343 648 2994 Fragments arranged by size: 2994 1425 648 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2266 3422 3781 3940 518 Size: 648 1100 1156 359 159 2042 Fragments arranged by size: 2042 1156 1100 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 2063 2155 2880 3557 4705 4950 1606 Size: 413 44 92 725 677 1148 245 2120 Fragments arranged by size: 2120 1148 725 677 413 245 92 44 NarI GG'CG_CC Cuts at: 2511 2511 Size: 5464 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4912 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2822 4367 1132 Size: 97 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5464 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2277 2277 Size: 5464 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 2468 3034 2468 Size: 566 4898 PstI C_TGCA'G Cuts at: 830 2024 2816 830 Size: 1194 792 3478 Fragments arranged by size: 3478 1194 792 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 PvuII CAG'CTG Cuts at: 2063 2155 2063 Size: 92 5372 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2020 2812 3947 4628 4819 826 Size: 219 975 792 1135 681 191 1471 Fragments arranged by size: 1471 1135 975 792 681 219 191 SfoI GGC'GCC Cuts at: 2512 2512 Size: 5464 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2791 1872 Size: 919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 2605 152 Size: 2453 3011 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5464 StyI C'CwwG_G Cuts at: 513 1065 2142 2280 2903 513 Size: 552 1077 138 623 3074 Fragments arranged by size: 3074 1077 623 552 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2201 2722 3491 3508 3661 3846 4291 Size: 1255 621 521 769 17 153 185 445 Cuts at: 4291 325 Size: 1498 Fragments arranged by size: 1498 1255 769 621 521 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2270 2634 3710 Size: 274 80 33 51 1443 293 364 1076 Cuts at: 3710 5422 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1076 364 293 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2389 2389 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2789 1870 Size: 919 4545 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI