Restriction map for
pFN21AE4038
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# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco47III Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KspI
MaeII MamI MfeI MluI MroI
MseI MunI MvnI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfuI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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Acc65I 614 GGTACC G^GTACC
AlwI 215 GGATC GGATC(4/5)
Asp718I 614 GGTACC G^GTACC
AspHI 304 GAGCAC G[AT]GC[AT]^C
AvaI 483 CTCGGG C^[CT]CG[GA]G
BalI 473 TGGCCA TGG^CCA
BanII 186 GAGCCC G[GA]GC[CT]^C
BbeI 505 GGCGCC GGCGC^C
BbsI 72 GAAGAC GAAGAC(2/6)
BlpI 53 GCTCAGC GC^T[ACGT]AGC
BpmI 301 CTGGAG CTGGAG(16/14)
Bpu1102I 53 GCTCAGC GC^T[ACGT]AGC
BpuAI 72 GAAGAC GAAGAC(2/6)
BseRI 667 CTCCTC GAGGAG(10/8)
BsiEI 202 CGGCCG CG[GA][CT]^CG
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
BsmAI 748 GAGAC GTCTC(1/5)
BsoBI 483 CTCGGG C^[CT]CG[GA]G
BsrFI 200 ACCGGC [GA]^CCGG[CT]
BsrI 308 ACTGG ACTGG(1/-1)
BssSI 223 CACGAG CACGAG(-5/-1)
BstAPI 212 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 317 CATCC GGATG(2/0)
BstYI 215 GGATCT [GA]^GATC[CT]
CelII 53 GCTCAGC GC^T[ACGT]AGC
Cfr10I 200 ACCGGC [GA]^CCGG[CT]
DpnII 216 GATC ^GATC
DraII 271 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 597 GACACTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 202 CGGCCG C^GGCCG
EarI 574 CTCTTC CTCTTC(1/4)
EclXI 202 CGGCCG C^GGCCG
Eco52I 202 CGGCCG C^GGCCG
EcoO109I 271 GGGACCT [GA]G^G[ACGT]CC[CT]
FauI 501 GCGGG CCCGC(4/6)
FokI 317 CATCC GGATG(9/13)
HaeII 505 GGCGCC [GA]GCGC^[CT]
HapII 201 CCGG C^CGG
HgaI 642 GCGTC GACGC(5/10)
HincII 156 GTCAAC GT[CT]^[GA]AC
HindII 156 GTCAAC GT[CT]^[GA]AC
HpaII 201 CCGG C^CGG
KasI 505 GGCGCC G^GCGCC
KpnI 614 GGTACC GGTAC^C
Ksp632I 574 CTCTTC CTCTTC(1/4)
MaeIII 20 GTCAC ^GT[ACGT]AC
MboI 216 GATC ^GATC
MflI 215 GGATCT [GA]^GATC[CT]
MluNI 473 TGGCCA TGG^CCA
MscI 473 TGGCCA TGG^CCA
MslI 257 CATGTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 201 CCGG C^CGG
NarI 505 GGCGCC GG^CGCC
NdeII 216 GATC ^GATC
PpuMI 271 GGGACCT [GA]G^G[AT]CC[CT]
PstI 15 CTGCAG CTGCA^G
Sau3AI 216 GATC ^GATC
SfcI 15 CTGCAG C^T[GA][CT]AG
SfoI 505 GGCGCC GGC^GCC
SpeI 600 ACTAGT A^CTAGT
TfiI 663 GATTC G^A[AT]TC
Tsp45I 20 GTCAC ^GT[GC]AC
Tsp509I 285 AATT ^AATT
XcmI 377 CCAGGCCGTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 215 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 505 GGCGCC G[GA]^CG[CT]C
641 GGCGTC
BanI 505 GGCGCC G^G[CT][GA]CC
614 GGTACC
BfaI 97 CTAG C^TAG
601 CTAG
BglI 221 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
497 GCCTGCGGGGC
BmyI 186 GAGCCC G[AGT]GC[ACT]^C
304 GAGCAC
BsaHI 505 GGCGCC G[GA]^CG[CT]C
641 GGCGTC
BsmFI 271 GGGAC GGGAC(10/14)
280 GGGAC
Bsp1286I 186 GAGCCC G[AGT]GC[ACT]^C
304 GAGCAC
Bsp1407I 265 TGTACA T^GTACA
629 TGTACA
BspMI 23 ACCTGC ACCTGC(4/8)
518 ACCTGC
BsrGI 265 TGTACA T^GTACA
629 TGTACA
Cac8I 488 GCTGGC GC[ACGT]^[ACGT]GC
497 GCCTGC
CfoI 369 GCGC GCG^C
506 GCGC
DdeI 54 CTCAG C^T[ACGT]AG
397 CTGAG
DsaI 65 CCGTGG C^C[GA][CT]GG
509 CCGTGG
EaeI 202 CGGCCG [CT]^GGCC[GA]
473 TGGCCA
EcoT14I 137 CCAAGG C^C[AT][AT]GG
275 CCTTGG
HhaI 369 GCGC GCG^C
506 GCGC
Hin1I 505 GGCGCC G[GA]^CG[CT]C
641 GGCGTC
HinP1I 369 GCGC G^CGC
506 GCGC
MaeI 97 CTAG C^TAG
601 CTAG
MboII 72 GAAGA GAAGA(8/7)
575 TCTTC
PleI 171 GACTC GAGTC(4/5)
429 GACTC
PvuII 56 CAGCTG CAG^CTG
148 CAGCTG
SspBI 265 TGTACA T^GTACA
629 TGTACA
StyI 137 CCAAGG C^C[AT][AT]GG
275 CCTTGG
TaqI 365 TCGA T^CGA
624 TCGA
TthHB8I 365 TCGA T^CGA
624 TCGA
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BsaJI BsgI BsiYI BslI
BssKI BstNI Cfr13I EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MnlI
MspA1I MvaI MwoI NlaIII NlaIV
RsaI Sau96I ScrFI TseI TspRI