Restriction map for
pFN21AE4038
(Linear) (Six-base) MAPSORT of: KIEE4038.seq Check: 9890 from: 1 to: 759
KIEE4038.orf
With 182 enzymes: SgfI *
March 22, 2012 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 32 527 759
Size: 32 495 232
Fragments arranged by size:
495 232 32
Acc65I G'GTAC_C
Cuts at: 0 614 759
Size: 614 145
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 759
Size: 336 423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 759
Size: 40 719
AlwNI CAG_nnn'CTG
Cuts at: 0 14 153 218 335 759
Size: 14 139 65 117 424
Fragments arranged by size:
424 139 117 65 14
AvaI C'yCGr_G
Cuts at: 0 483 759
Size: 483 276
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 606 639 759
Size: 606 33 120
Fragments arranged by size:
606 120 33
BanI G'GyrC_C
Cuts at: 0 505 614 759
Size: 505 109 145
Fragments arranged by size:
505 145 109
BanII G_rGCy'C
Cuts at: 0 190 759
Size: 190 569
BbeI G_GCGC'C
Cuts at: 0 509 759
Size: 509 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 79 759
Size: 79 680
BglI GCCn_nnn'nGGC
Cuts at: 0 227 503 759
Size: 227 276 256
Fragments arranged by size:
276 256 227
BlpI GC'TnA_GC
Cuts at: 0 54 759
Size: 54 705
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 759
Size: 322 437
BsaHI Gr'CG_yC
Cuts at: 0 506 642 759
Size: 506 136 117
Fragments arranged by size:
506 136 117
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 658 759
Size: 658 101
BseYI C'CCAG_C
Cuts at: 0 413 714 759
Size: 413 301 45
Fragments arranged by size:
413 301 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 44 231 505 759
Size: 30 14 187 274 254
Fragments arranged by size:
274 254 187 30 14
BsiEI CG_ry'CG
Cuts at: 0 205 759
Size: 205 554
BsiHKAI G_wGCw'C
Cuts at: 0 308 759
Size: 308 451
Bsp1286I G_dGCh'C
Cuts at: 0 190 308 759
Size: 190 118 451
Fragments arranged by size:
451 190 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 527 759
Size: 32 495 232
Fragments arranged by size:
495 232 32
BsrFI r'CCGG_y
Cuts at: 0 200 759
Size: 200 559
BsrGI T'GTAC_A
Cuts at: 0 265 629 759
Size: 265 364 130
Fragments arranged by size:
364 265 130
BssSI C'ACGA_G
Cuts at: 0 223 759
Size: 223 536
BstAPI GCAn_nnn'nTGC
Cuts at: 0 218 759
Size: 218 541
BstYI r'GATC_y
Cuts at: 0 215 759
Size: 215 544
BtgI C'CryG_G
Cuts at: 0 65 509 759
Size: 65 444 250
Fragments arranged by size:
444 250 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 603 759
Size: 603 156
EaeI y'GGCC_r
Cuts at: 0 202 473 759
Size: 202 271 286
Fragments arranged by size:
286 271 202
EagI C'GGCC_G
Cuts at: 0 202 759
Size: 202 557
EarI CTCTTCn'nnn_
Cuts at: 0 580 759
Size: 580 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 336 759
Size: 322 14 423
Fragments arranged by size:
423 322 14
EcoO109I rG'GnC_Cy
Cuts at: 0 272 759
Size: 272 487
HaeII r_GCGC'y
Cuts at: 0 509 759
Size: 509 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 235 267 273 305 759
Size: 235 32 6 32 454
Fragments arranged by size:
454 235 32 32 6
HincII GTy'rAC
Cuts at: 0 158 759
Size: 158 601
KasI G'GCGC_C
Cuts at: 0 505 759
Size: 505 254
KpnI G_GTAC'C
Cuts at: 0 618 759
Size: 618 141
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 625 759
Size: 528 97 134
Fragments arranged by size:
528 134 97
MscI TGG'CCA
Cuts at: 0 475 759
Size: 475 284
MslI CAynn'nnrTG
Cuts at: 0 261 759
Size: 261 498
MspA1I CmG'CkG
Cuts at: 0 14 58 150 759
Size: 14 44 92 609
Fragments arranged by size:
609 92 44 14
NarI GG'CG_CC
Cuts at: 0 506 759
Size: 506 253
PpuMI rG'GwC_Cy
Cuts at: 0 272 759
Size: 272 487
PsiI TTA'TAA
Cuts at: 0 463 759
Size: 463 296
PstI C_TGCA'G
Cuts at: 0 19 759
Size: 19 740
PvuII CAG'CTG
Cuts at: 0 58 150 759
Size: 58 92 609
Fragments arranged by size:
609 92 58
SfcI C'TryA_G
Cuts at: 0 15 759
Size: 15 744
SfoI GGC'GCC
Cuts at: 0 507 759
Size: 507 252
SpeI A'CTAG_T
Cuts at: 0 600 759
Size: 600 159
StyI C'CwwG_G
Cuts at: 0 137 275 759
Size: 137 138 484
Fragments arranged by size:
484 138 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 196 717 759
Size: 196 521 42
Fragments arranged by size:
521 196 42
TatI w'GTAC_w
Cuts at: 0 265 629 759
Size: 265 364 130
Fragments arranged by size:
364 265 130
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 759
Size: 384 375
Enzymes that do cut:
AarI Acc65I AcuI AlfI AlwNI AvaI BaeI BanI
BanII BbeI BbsI BglI BlpI BpmI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BsrGI
BssSI BstAPI BstYI BtgI DrdI EaeI EagI EarI
Eco57MI EcoO109I HaeII Hin4I HincII KasI KpnI MmeI
MscI MslI MspA1I NarI PpuMI PsiI PstI PvuII
SfcI SfoI SpeI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BbvCI BcgI BciVI BclI BfrBI BglII
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI