ASB5 ORF clone pFN21AE3982

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Halotag ORF Clone pFN21AE3982

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Clone info.

Species

human

Clone Name

pFN21AE3982 Sequence (with vector) Restriction Map

Product ID

FHC11065

ORF Sequence

DNA sequence (987 bp)
Protein sequence (329 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3982

Symbol

ASB5

Alias : ASB-5

Description

ankyrin repeat and SOCS box containing 5

Original Clone ID

cp93982

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 987 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 329 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS3827.1 1.7e-106 100.0 ASB5 CCDS14164.1 4.4e-55 53.8 ASB11 CCDS35209.1 5.7e-46 54.9 ASB11 CCDS35208.1 5.7e-41 53.1 ASB9 CCDS14163.1 6.6e-36 53.4 ASB9 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan NULL 136 151 PR01415 NULL NULL 248 262 PR01415 NULL HMMPfam IPR002110 71 100 PF00023 Ankyrin repeat IPR002110 103 133 PF00023 Ankyrin repeat IPR002110 135 160 PF00023 Ankyrin repeat IPR002110 234 263 PF00023 Ankyrin repeat IPR001496 290 329 PF07525 SOCS protein HMMSmart IPR002110 69 98 SM00248 Ankyrin repeat IPR002110 102 131 SM00248 Ankyrin repeat IPR002110 135 164 SM00248 Ankyrin repeat IPR002110 167 196 SM00248 Ankyrin repeat IPR002110 200 229 SM00248 Ankyrin repeat IPR002110 232 261 SM00248 Ankyrin repeat IPR001496 290 329 SM00969 SOCS protein ProfileScan IPR002110 69 101 PS50088 Ankyrin repeat IPR020683 69 283 PS50297 Ankyrin repeat-containing domain IPR002110 102 134 PS50088 Ankyrin repeat IPR002110 135 167 PS50088 Ankyrin repeat IPR002110 232 264 PS50088 Ankyrin repeat IPR001496 278 329 PS50225 SOCS protein
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 19 FTILSLFCFKLFVKISLAILSHF 41 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_543150 329 615050 100.0 100.0 XP_005262816 329 615050 100.0 100.0 XP_011529919 276 615050 100.0 80.2 XP_011529918 284 615050 100.0 80.2 NP_543149 323 300626 53.8 98.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3982 Download

>KIEE3982  987 bp
ATGTCGGTGTTAGAAGAAAATCGGCCGTTTGCTCAACAATTATCCAATGTCTACTTTACA
ATACTTTCGCTGTTCTGTTTTAAGCTTTTTGTGAAAATCAGCCTTGCCATCCTCAGTCAT
TTCTACATAGTGAAAGGCAACCGCAAGGAAGCGGCAAGGATAGCAGCTGAATTTTATGGA
GTAACCCAAGGACAAGGTTCCTGGGCAGATCGATCACCACTACATGAAGCAGCAAGTCAA
GGTCGCCTTCTTGCTCTGAGAACATTATTATCACAGGGTTATAATGTAAATGCAGTAACC
TTAGACCATGTCACCCCATTGCACGAAGCCTGCCTTGGAGATCACGTGGCATGTGCCAGA
ACTCTGCTGGAAGCAGGAGCTAATGTAAATGCAATCACGATAGATGGCGTGACTCCGTTA
TTCAACGCATGCTCCCAAGGCAGTCCAAGCTGTGCAGAGCTGCTTCTGGAGTATGGTGCC
AAAGCCCAGCTGGAGTCATGTCTTCCATCCCCAACGCATGAGGCCGCCAGTAAAGGTCAC
CATGAATGTCTTGACATCCTGATATCCTGGGGCATAGATGTTGACCAAGAAATTCCTCAT
TTGGGAACTCCTCTCTATGTAGCTTGTATGTCACAGCAATTCCATTGCATCTGGAAGCTT
CTTTATGCTGGTGCTGACGTACAGAAAGGCAAATATTGGGATACTCCATTACATGCTGCT
GCTCAACAATCCAGCACAGAAATTGTAAACTTACTGCTAGAATTTGGAGCAGATATCAAT
GCCAAAAATACAGAGCTTCTGCGACCTATAGATGTAGCTACGTCTAGCAGTATGGTGGAA
AGAATATTGCTTCAACATGAAGCTACTCCAAGCTCTCTTTACCAACTTTGCCGACTCTGT
ATCCGAAGCTACATAGGAAAACCAAGATTGCACCTTATCCCACAACTCCAGCTGCCAACG
TTACTGAAGAATTTCTTACAGTATCGA

ORF Protein

Cloned ORF protein sequence for pFN21AE3982 Download

>KIEE3982  329 aa
MSVLEENRPFAQQLSNVYFTILSLFCFKLFVKISLAILSHFYIVKGNRKEAARIAAEFYG
VTQGQGSWADRSPLHEAASQGRLLALRTLLSQGYNVNAVTLDHVTPLHEACLGDHVACAR
TLLEAGANVNAITIDGVTPLFNACSQGSPSCAELLLEYGAKAQLESCLPSPTHEAASKGH
HECLDILISWGIDVDQEIPHLGTPLYVACMSQQFHCIWKLLYAGADVQKGKYWDTPLHAA
AQQSSTEIVNLLLEFGADINAKNTELLRPIDVATSSSMVERILLQHEATPSSLYQLCRLC
IRSYIGKPRLHLIPQLQLPTLLKNFLQYR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3982 Download

>pFN21AE3982  5692 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCGGTGTTAGAAGAAAATCGGCCGTTTGCTCA
ACAATTATCCAATGTCTACTTTACAATACTTTCGCTGTTCTGTTTTAAGCTTTTTGTGAA
AATCAGCCTTGCCATCCTCAGTCATTTCTACATAGTGAAAGGCAACCGCAAGGAAGCGGC
AAGGATAGCAGCTGAATTTTATGGAGTAACCCAAGGACAAGGTTCCTGGGCAGATCGATC
ACCACTACATGAAGCAGCAAGTCAAGGTCGCCTTCTTGCTCTGAGAACATTATTATCACA
GGGTTATAATGTAAATGCAGTAACCTTAGACCATGTCACCCCATTGCACGAAGCCTGCCT
TGGAGATCACGTGGCATGTGCCAGAACTCTGCTGGAAGCAGGAGCTAATGTAAATGCAAT
CACGATAGATGGCGTGACTCCGTTATTCAACGCATGCTCCCAAGGCAGTCCAAGCTGTGC
AGAGCTGCTTCTGGAGTATGGTGCCAAAGCCCAGCTGGAGTCATGTCTTCCATCCCCAAC
GCATGAGGCCGCCAGTAAAGGTCACCATGAATGTCTTGACATCCTGATATCCTGGGGCAT
AGATGTTGACCAAGAAATTCCTCATTTGGGAACTCCTCTCTATGTAGCTTGTATGTCACA
GCAATTCCATTGCATCTGGAAGCTTCTTTATGCTGGTGCTGACGTACAGAAAGGCAAATA
TTGGGATACTCCATTACATGCTGCTGCTCAACAATCCAGCACAGAAATTGTAAACTTACT
GCTAGAATTTGGAGCAGATATCAATGCCAAAAATACAGAGCTTCTGCGACCTATAGATGT
AGCTACGTCTAGCAGTATGGTGGAAAGAATATTGCTTCAACATGAAGCTACTCCAAGCTC
TCTTTACCAACTTTGCCGACTCTGTATCCGAAGCTACATAGGAAAACCAAGATTGCACCT
TATCCCACAACTCCAGCTGCCAACGTTACTGAAGAATTTCTTACAGTATCGAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT