Restriction map for
pFN21AE3982
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BamHI BanII BbeI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
Cfr13I CpoI DraI DraII DraIII
DrdI DsaI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO109I EcoRI EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI StuI SwaI
TfiI TspRI Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 49 GTCTAC GT^[AC][GT]AC
AclI 957 AACGTT AA^CGTT
AfaI 679 GTAC GT^AC
Asp700I 966 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 304 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 475 GGTGCC G^G[CT][GA]CC
BbrPI 343 CACGTG CAC^GTG
BbsI 500 GTCTTC GAAGAC(2/6)
BpuAI 500 GTCTTC GAAGAC(2/6)
BsaAI 343 CACGTG [CT]AC^GT[GA]
BseRI 608 CTCCTC GAGGAG(10/8)
BsgI 452 GTGCAG GTGCAG(16/14)
BsiEI 22 CGGCCG CG[GA][CT]^CG
BsiYI 595 CCTCATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 595 CCTCATTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI 209 ATCGAT AT^CGAT
BsrI 527 CCAGT ACTGG(1/-1)
BstEII 535 GGTCACC G^GT[ACGT]ACC
BstPI 535 GGTCACC G^GT[ACGT]ACC
ClaI 209 ATCGAT AT^CGAT
EaeI 22 CGGCCG [CT]^GGCC[GA]
EagI 22 CGGCCG C^GGCCG
EclXI 22 CGGCCG C^GGCCG
Eco52I 22 CGGCCG C^GGCCG
EcoO65I 535 GGTCACC G^GT[ACGT]ACC
HincII 580 GTTGAC GT[CT]^[GA]AC
HindII 580 GTTGAC GT[CT]^[GA]AC
MseI 80 TTAA T^TAA
PflFI 304 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 343 CACGTG CAC^GTG
PmlI 343 CACGTG CAC^GTG
Psp1406I 957 AACGTT AA^CGTT
RsaI 679 GTAC GT^AC
SfaNI 647 GCATC GCATC(5/9)
SfcI 806 CTATAG C^T[GA][CT]AG
SphI 427 GCATGC GCATG^C
Tru9I 80 TTAA T^TAA
Tth111I 304 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 966 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BciVI 699 GGATAC GTATCC(6/5)
899 GTATCC
BfaI 757 CTAG C^TAG
824 CTAG
BsrDI 317 CATTGC GCAATG(2/0)
643 CATTGC
BssKI 200 CCTGG ^CC[ACGT]GG
566 CCTGG
BstNI 200 CCTGG CC^[AT]GG
566 CCTGG
Cac8I 328 GCCTGC GC[ACGT]^[ACGT]GC
427 GCATGC
EcoRII 200 CCTGG ^CC[AT]GG
566 CCTGG
EcoRV 561 GATATC GAT^ATC
772 GATATC
HaeIII 23 GGCC GG^CC
522 GGCC
HindIII 82 AAGCTT A^AGCTT
655 AAGCTT
MaeI 757 CTAG C^TAG
824 CTAG
MvaI 200 CCTGG CC^[AT]GG
566 CCTGG
NlaIV 196 GGTTCC GG[ACGT]^[ACGT]CC
475 GGTGCC
ScrFI 200 CCTGG CC^[ACGT]GG
566 CCTGG
SspI 692 AATATT AAT^ATT
843 AATATT
TaqI 210 TCGA T^CGA
984 TCGA
TthHB8I 210 TCGA T^CGA
984 TCGA
XcmI 356 CCAGAACTCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
479 CCAAAGCCCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BpmI BsaJI BstF5I DdeI DpnII
EcoT14I Fnu4HI FokI HinfI HphI
ItaI MaeII MaeIII MboI MboII
MnlI MspA1I MwoI NdeII NlaIII
NspI PleI PvuII Sau3AI StyI
TseI Tsp45I Tsp509I