(Circular) (Six-base) MAPSORT of: pFN21AE3982.seq Check: 3198 from: 1 to: 5692 pFN21AE3982 5692 bp With 182 enzymes: SgfI * March 28, 2012 11:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2055 3035 1937 Size: 118 980 4594 Fragments arranged by size: 4594 980 118 Acc65I G'GTAC_C Cuts at: 3013 3013 Size: 5692 AclI AA'CG_TT Cuts at: 1563 2963 3819 4192 1563 Size: 1400 856 373 3063 Fragments arranged by size: 3063 1400 856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2990 3755 5139 5407 1632 Size: 1358 765 1384 268 1917 Fragments arranged by size: 1917 1384 1358 765 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 3435 5007 3435 Size: 1572 4120 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 2174 2595 2765 2974 3001 3227 3379 5488 Size: 681 421 170 209 27 226 152 2109 Cuts at: 5488 1493 Size: 1697 Fragments arranged by size: 2109 1697 681 421 226 209 170 152 27 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 3017 1870 Size: 73 1074 4545 Fragments arranged by size: 4545 1074 73 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2480 3013 4468 618 Size: 325 217 627 693 533 1455 1842 Fragments arranged by size: 1842 1455 693 627 533 325 217 BanII G_rGCy'C Cuts at: 1391 3011 1391 Size: 1620 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2498 928 Size: 971 599 4122 Fragments arranged by size: 4122 971 599 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2698 2915 3584 4794 2698 Size: 217 669 1210 3596 Fragments arranged by size: 3596 1210 669 217 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 3753 4909 1790 Size: 1963 1156 2573 Fragments arranged by size: 2573 1963 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3423 4381 442 Size: 416 53 83 399 24 2006 958 1753 Fragments arranged by size: 2006 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2516 2936 4352 1239 Size: 1253 24 420 1416 2579 Fragments arranged by size: 2579 1416 1253 420 24 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2350 3483 493 Size: 711 1146 1133 2702 Fragments arranged by size: 2702 1146 1133 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4125 4523 4797 4944 5355 4125 Size: 398 274 147 411 4462 Fragments arranged by size: 4462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 2373 2403 2420 Size: 30 165 30 931 30 9 30 17 Cuts at: 2420 2450 1178 Size: 30 4420 Fragments arranged by size: 4420 931 165 30 30 30 30 30 17 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2604 1186 Size: 1418 4274 BseYI C'CCAG_C Cuts at: 2490 4895 5391 2490 Size: 2405 496 2791 Fragments arranged by size: 2791 2405 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2478 1163 Size: 573 742 4377 Fragments arranged by size: 4377 742 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2030 3142 3903 4931 1540 Size: 275 186 29 1112 761 1028 2301 Fragments arranged by size: 2301 1112 1028 761 275 186 29 BsiHKAI G_wGCw'C Cuts at: 3753 3838 4909 3753 Size: 85 1071 4536 Fragments arranged by size: 4536 1071 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3011 3753 3838 4909 1391 Size: 399 1221 742 85 1071 2174 Fragments arranged by size: 2174 1221 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3047 844 Size: 2203 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2321 2647 4187 4361 65 Size: 1183 1073 326 1540 174 1396 Fragments arranged by size: 1540 1396 1183 1073 326 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4336 4523 1700 Size: 110 2526 187 2869 Fragments arranged by size: 2869 2526 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 3752 4764 3752 Size: 1012 4680 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 2540 1184 Size: 1356 4336 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5140 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 3208 4002 4022 3208 Size: 794 20 4878 Fragments arranged by size: 4878 794 20 ClaI AT'CG_AT Cuts at: 1554 2215 1554 Size: 661 5031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2027 3139 4028 Size: 54 1425 50 275 18 197 1112 889 Cuts at: 4028 5657 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1112 889 275 197 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2027 3139 1537 Size: 275 215 1112 4090 Fragments arranged by size: 4090 1112 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 3622 1353 Size: 2269 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4279 4653 4799 387 Size: 1064 138 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2516 2936 2990 3755 4352 5139 Size: 393 860 24 420 54 765 597 787 Cuts at: 5139 5407 1239 Size: 268 1524 Fragments arranged by size: 1524 860 787 765 597 420 393 268 54 24 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 EcoRV GAT'ATC Cuts at: 2568 2779 2568 Size: 211 5481 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2267 2354 2995 2267 Size: 87 641 4964 Fragments arranged by size: 4964 641 87 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2763 2795 2889 2921 4333 Size: 32 135 32 1191 32 94 32 1412 Cuts at: 4333 4365 4407 4439 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1412 1191 135 94 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2587 3036 3282 669 Size: 988 281 649 449 246 3079 Fragments arranged by size: 3079 988 649 449 281 246 HindIII A'AGCT_T Cuts at: 748 2087 2660 748 Size: 1339 573 3780 Fragments arranged by size: 3780 1339 573 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 3017 3017 Size: 5692 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4806 4990 1305 Size: 3501 184 2007 Fragments arranged by size: 3501 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3650 4009 4168 518 Size: 648 843 1641 359 159 2042 Fragments arranged by size: 2042 1641 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2171 2494 2956 3108 3785 4933 5178 1606 Size: 565 323 462 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 565 462 323 245 152 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5140 NdeI CA'TA_TG Cuts at: 387 387 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 2358 2436 2720 3050 4595 1132 Size: 97 1129 78 284 330 1545 2229 Fragments arranged by size: 2229 1545 1129 330 284 97 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PmlI CAC'GTG Cuts at: 2350 2350 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 2286 3262 2286 Size: 976 4716 PstI C_TGCA'G Cuts at: 830 3044 830 Size: 2214 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 PvuII CAG'CTG Cuts at: 2171 2494 2956 2171 Size: 323 462 4907 Fragments arranged by size: 4907 462 323 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2811 3040 4175 4856 5047 826 Size: 219 1766 229 1135 681 191 1471 Fragments arranged by size: 1766 1471 1135 681 229 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 2436 3050 1132 Size: 1304 614 3774 Fragments arranged by size: 3774 1304 614 SspI AAT'ATT Cuts at: 5 52 2699 2850 3616 5 Size: 47 2647 151 766 2081 Fragments arranged by size: 2647 2081 766 151 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2191 2338 2440 3131 513 Size: 552 1126 147 102 691 3074 Fragments arranged by size: 3074 1126 691 552 147 102 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3719 3736 3889 4074 4519 325 Size: 1255 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2334 2366 2628 2660 5652 5684 2334 Size: 32 262 32 2992 32 2342 Fragments arranged by size: 2992 2342 262 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2312 1316 Size: 996 4696 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 2491 2368 Size: 123 5569 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 2975 3821 2975 Size: 846 4846 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI