PCMTD2 ORF clone pFN21AE3755

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3755

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Clone info.

Species

human

Clone Name

pFN21AE3755 Sequence (with vector) Restriction Map

Product ID

FHC27119

ORF Sequence

DNA sequence (1083 bp)
Protein sequence (361 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3755

Symbol

PCMTD2

Alias : C20orf36

Description

protein-L-isoaspartate (D-aspartate) O-methyltransferase domain containing 2, transcript variant 1

Original Clone ID

cp93755

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1083 bp
Restriction map A Restriction map B

Features of the protein sequence

Description

Length: 361 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13559.1 6.6e-144 100.0 PCMTD2 CCDS6148.1 1.3e-95 67.5 PCMTD1 CCDS46631.1 2.4e-75 92.5 PCMTD2 CCDS69480.1 9.1e-45 55.1 PCMTD1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000682 10 220 PF01135 Protein-L-isoaspartate(D-aspartate) O-methyltransferase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3755 Download

>KIEE3755  1083 bp
ATGGGCGGTGCTGTGAGTGCTGGTGAAGACAATGATGAGCTGATAGATAATTTGAAAGAA
GCACAGTATATCCGGACTGAGCTGGTAGAGCAGGCTTTCAGAGCTATCGATCGTGCAGAC
TATTATCTTGAAGAATTTAAAGAAAATGCTTATAAAGACTTGGCATGGAAGCATGGAAAC
ATTCACCTCTCAGCCCCGTGCATCTACTCGGAGGTGATGGAAGCCCTAGATCTGCAGCCT
GGACTCTCGTTTCTGAACCTGGGCAGTGGCACTGGGTATCTCAGCTCCATGGTGGGCCTC
ATTCTAGGTCCTTTTGGTGTGAACCATGGGGTGGAACTTCACTCAGATGTGATAGAGTAT
GCAAAGCAGAAACTGGACTTCTTCATCAGAACAAGTGATAGTTTTGACAAGTTTGACTTC
TGTGAACCTTCCTTTGTTACTGGGAATTGCCTGGAGATTTCTCCGGATTGTTCTCAGTAT
GATCGTGTATACTGTGGGGCTGGCGTGCAGAAAGAGCATGAAGAGTACATGAAGAATCTT
CTCAAAGTGGGAGGGATCCTTGTCATGCCACTGGAAGAGAAGTTGACTAAGATAACACGC
ACAGGTCCTTCAGCTTGGGAAACCAAAAAGATTCTTGCTGTTTCTTTTGCTCCTCTGATC
CAGCCCTGCCATTCAGAGTCAGGAAAATCAAGACTTGTCCAGTTACCACCAGTGGCAGTT
CGCAGCCTCCAGGACTTGGCTCGCATCGCCATCCGGGGCACCATTAAAAAGATTATTCAT
CAGGAAACTGTGAGCAAAAACGGAAACGGACTAAAGAACACCCCCAGGTTCAAACGAAGG
AGAGTTCGCCGCCGTCGAATGGAAACGATTGTCTTTTTGGACAAAGAAGTCTTTGCCAGT
CGGATTTCCAACCCCTCAGATGACAACAGCTGTGAAGACTTGGAAGAGGAACGGAGGGAA
GAAGAAGAGAAGACCCCGCCGGAAACAAAGCCAGACCCCCCAGTGAACTTCCTACGCCAG
AAGGTCCTGAGCCTCCCTCTGCCAGATCCCCTGAAATACTACTTGCTTTATTACAGAGAA
AAA

ORF Protein

Cloned ORF protein sequence for pFN21AE3755 Download

>KIEE3755  361 aa
MGGAVSAGEDNDELIDNLKEAQYIRTELVEQAFRAIDRADYYLEEFKENAYKDLAWKHGN
IHLSAPCIYSEVMEALDLQPGLSFLNLGSGTGYLSSMVGLILGPFGVNHGVELHSDVIEY
AKQKLDFFIRTSDSFDKFDFCEPSFVTGNCLEISPDCSQYDRVYCGAGVQKEHEEYMKNL
LKVGGILVMPLEEKLTKITRTGPSAWETKKILAVSFAPLIQPCHSESGKSRLVQLPPVAV
RSLQDLARIAIRGTIKKIIHQETVSKNGNGLKNTPRFKRRRVRRRRMETIVFLDKEVFAS
RISNPSDDNSCEDLEEERREEEEKTPPETKPDPPVNFLRQKVLSLPLPDPLKYYLLYYRE
K

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3755 Download

>pFN21AE3755  5788 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGCGGTGCTGTGAGTGCTGGTGAAGACAATGA
TGAGCTGATAGATAATTTGAAAGAAGCACAGTATATCCGGACTGAGCTGGTAGAGCAGGC
TTTCAGAGCTATCGATCGTGCAGACTATTATCTTGAAGAATTTAAAGAAAATGCTTATAA
AGACTTGGCATGGAAGCATGGAAACATTCACCTCTCAGCCCCGTGCATCTACTCGGAGGT
GATGGAAGCCCTAGATCTGCAGCCTGGACTCTCGTTTCTGAACCTGGGCAGTGGCACTGG
GTATCTCAGCTCCATGGTGGGCCTCATTCTAGGTCCTTTTGGTGTGAACCATGGGGTGGA
ACTTCACTCAGATGTGATAGAGTATGCAAAGCAGAAACTGGACTTCTTCATCAGAACAAG
TGATAGTTTTGACAAGTTTGACTTCTGTGAACCTTCCTTTGTTACTGGGAATTGCCTGGA
GATTTCTCCGGATTGTTCTCAGTATGATCGTGTATACTGTGGGGCTGGCGTGCAGAAAGA
GCATGAAGAGTACATGAAGAATCTTCTCAAAGTGGGAGGGATCCTTGTCATGCCACTGGA
AGAGAAGTTGACTAAGATAACACGCACAGGTCCTTCAGCTTGGGAAACCAAAAAGATTCT
TGCTGTTTCTTTTGCTCCTCTGATCCAGCCCTGCCATTCAGAGTCAGGAAAATCAAGACT
TGTCCAGTTACCACCAGTGGCAGTTCGCAGCCTCCAGGACTTGGCTCGCATCGCCATCCG
GGGCACCATTAAAAAGATTATTCATCAGGAAACTGTGAGCAAAAACGGAAACGGACTAAA
GAACACCCCCAGGTTCAAACGAAGGAGAGTTCGCCGCCGTCGAATGGAAACGATTGTCTT
TTTGGACAAAGAAGTCTTTGCCAGTCGGATTTCCAACCCCTCAGATGACAACAGCTGTGA
AGACTTGGAAGAGGAACGGAGGGAAGAAGAAGAGAAGACCCCGCCGGAAACAAAGCCAGA
CCCCCCAGTGAACTTCCTACGCCAGAAGGTCCTGAGCCTCCCTCTGCCAGATCCCCTGAA
ATACTACTTGCTTTATTACAGAGAAAAAGTTTAAACGAATTCGGGCTCGGTACCCGGGGA
TCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGG
AAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCG
CTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAG
TGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATA
AGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGG
GAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTC
TAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTC
ATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCA
GGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACAT
TCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAA
AGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTT
TGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAG
TTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGT
TTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCG
GTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAG
AATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTA
AGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTG
ACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTA
ACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGAC
ACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTT
ACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCA
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAG
CGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTA
GTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAG
ATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAAC
CGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGC
TGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTC
AGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCC
TCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTT
CGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCG
TTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTAT
CCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGT
GGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGC
CAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTA
GCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAG
ATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGA
TTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATA
CAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTT
CAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGG
TTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCT
GTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGG
TAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACG
ATGCGTCCGGCGTAGAGGATCGAGATCT