(Circular) (Six-base) MAPSORT of: pFN21AE3755.seq Check: 8942 from: 1 to: 5788 pFN21AE3755 5788 bp With 182 enzymes: SgfI * March 28, 2012 12:04 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3598 278 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2493 3131 1937 Size: 556 638 4594 Fragments arranged by size: 4594 638 556 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2598 3851 5235 5503 1632 Size: 966 1253 1384 268 1917 Fragments arranged by size: 1917 1384 1253 966 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 4687 5664 1203 Size: 3484 977 1327 Fragments arranged by size: 3484 1327 977 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 2377 3531 5103 2377 Size: 1154 1572 3062 Fragments arranged by size: 3062 1572 1154 ApaLI G'TGCA_C Cuts at: 3845 5001 3845 Size: 1156 4632 ApoI r'AATT_y Cuts at: 1493 2138 3097 3323 3475 5584 1493 Size: 645 959 226 152 2109 1697 Fragments arranged by size: 2109 1697 959 645 226 152 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 3113 1870 Size: 73 1170 4545 Fragments arranged by size: 4545 1170 73 BamHI G'GATC_C Cuts at: 2559 3118 3485 2559 Size: 559 367 4862 Fragments arranged by size: 4862 559 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2762 3109 4564 618 Size: 325 217 627 975 347 1455 1842 Fragments arranged by size: 1842 1455 975 627 347 325 217 BanII G_rGCy'C Cuts at: 1391 3107 1391 Size: 1716 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2946 2981 928 Size: 971 138 909 35 3735 Fragments arranged by size: 3735 971 909 138 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 1548 Size: 5788 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2233 5783 2233 Size: 3550 2238 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2765 3849 5005 1790 Size: 975 1084 1156 2573 Fragments arranged by size: 2573 1156 1084 975 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2286 2454 2999 Size: 416 53 83 399 24 869 168 545 Cuts at: 2999 3519 4477 442 Size: 520 958 1753 Fragments arranged by size: 1753 958 869 545 520 416 399 168 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3057 3057 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2477 2717 4448 1239 Size: 1238 240 1731 2579 Fragments arranged by size: 2579 1731 1238 240 Bpu10I CC'TnA_GC Cuts at: 3032 3032 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4451 882 Size: 617 2952 2219 Fragments arranged by size: 2952 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3579 493 Size: 711 2375 2702 Fragments arranged by size: 2702 2375 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3595 3977 4612 275 Size: 53 83 186 936 2062 382 635 1451 Fragments arranged by size: 2062 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2076 2467 4221 4619 4893 5040 5451 2076 Size: 391 1754 398 274 147 411 2413 Fragments arranged by size: 2413 1754 411 398 391 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2646 1186 Size: 1460 4328 BseYI C'CCAG_C Cuts at: 4991 5487 4991 Size: 496 5292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2139 2531 1163 Size: 573 403 392 4420 Fragments arranged by size: 4420 573 403 392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 3238 3999 5027 1540 Size: 275 186 115 1122 761 1028 2301 Fragments arranged by size: 2301 1122 1028 761 275 186 115 BsiHKAI G_wGCw'C Cuts at: 3849 3934 5005 3849 Size: 85 1071 4632 Fragments arranged by size: 4632 1071 85 BsmI GAATG_Cn' Cuts at: 3299 3392 3299 Size: 93 5695 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2765 3107 3849 3934 5005 1391 Size: 399 975 342 742 85 1071 2174 Fragments arranged by size: 2174 1071 975 742 399 342 85 BspEI T'CCGG_A Cuts at: 2076 2467 5451 2076 Size: 391 2984 2413 Fragments arranged by size: 2984 2413 391 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 844 Size: 2299 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3675 1394 Size: 429 1852 3507 Fragments arranged by size: 3507 1852 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4432 4619 1700 Size: 110 2622 187 2869 Fragments arranged by size: 2869 2622 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5788 BssSI C'ACGA_G Cuts at: 3848 4860 3848 Size: 1012 4776 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5788 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2233 2559 3049 3118 3485 3870 3887 5339 Size: 261 326 490 69 367 385 17 1452 Cuts at: 5339 5425 5437 5783 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 490 385 367 346 326 261 86 69 17 12 BstZ17I GTA'TAC Cuts at: 2494 2494 Size: 5788 BtgI C'CryG_G Cuts at: 513 1065 2004 2292 2329 513 Size: 552 939 288 37 3972 Fragments arranged by size: 3972 939 552 288 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2734 487 Size: 683 56 1508 3541 Fragments arranged by size: 3541 1508 683 56 BtsI GCAGTG_nn' Cuts at: 2275 3304 4098 4118 2275 Size: 1029 794 20 3945 Fragments arranged by size: 3945 1029 794 20 ClaI AT'CG_AT Cuts at: 1554 2112 1554 Size: 558 5230 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 2143 3092 2143 Size: 949 4839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 4795 809 Size: 507 1575 1904 1802 Fragments arranged by size: 1904 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3235 4124 5753 Size: 54 1425 50 275 18 1405 889 1629 Cuts at: 5753 8 Size: 43 Fragments arranged by size: 1629 1425 1405 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 2574 2943 2964 3718 1353 Size: 1167 54 369 21 754 3423 Fragments arranged by size: 3423 1167 754 369 54 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2477 2598 2717 3851 4448 5235 5503 Size: 393 845 121 119 1134 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 1134 845 787 597 393 268 121 119 EcoO109I rG'GnC_Cy Cuts at: 2312 2609 3028 2312 Size: 297 419 5072 Fragments arranged by size: 5072 419 297 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2437 2437 Size: 5788 FspI TGC'GCA Cuts at: 4294 4294 Size: 5788 HaeII r_GCGC'y Cuts at: 1678 4935 1678 Size: 3257 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2767 2799 4429 4461 4503 Size: 32 135 32 1195 32 1630 32 42 Cuts at: 4503 4535 1373 Size: 32 2626 Fragments arranged by size: 2626 1630 1195 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2589 3132 3378 669 Size: 988 281 651 543 246 3079 Fragments arranged by size: 3079 988 651 543 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2885 2937 4902 5086 1305 Size: 1580 52 1965 184 2007 Fragments arranged by size: 2007 1965 1580 184 52 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3966 Fragments arranged by size: 3966 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2349 3746 4105 4264 518 Size: 648 1183 1397 359 159 2042 Fragments arranged by size: 2042 1397 1183 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2934 3204 3881 5029 5274 1606 Size: 1328 270 677 1148 245 2120 Fragments arranged by size: 2120 1328 1148 677 270 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2292 2329 513 Size: 552 939 288 37 3972 Fragments arranged by size: 3972 939 552 288 37 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 2740 1108 Size: 1227 405 4156 Fragments arranged by size: 4156 1227 405 PfoI T'CCnGG_A Cuts at: 2733 2733 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2312 2609 3028 2312 Size: 297 419 5072 Fragments arranged by size: 5072 419 297 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 2157 3358 2157 Size: 1201 4587 PstI C_TGCA'G Cuts at: 830 2241 3140 830 Size: 1411 899 3478 Fragments arranged by size: 3478 1411 899 PvuI CG_AT'CG Cuts at: 2001 2116 2001 Size: 115 5673 PvuII CAG'CTG Cuts at: 2934 2934 Size: 5788 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SfcI C'TryA_G Cuts at: 826 1045 2237 3136 4271 4952 5143 826 Size: 219 1192 899 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 899 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3892 4793 5055 820 Size: 197 926 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5788 StyI C'CwwG_G Cuts at: 513 1065 2004 2292 2329 3227 513 Size: 552 939 288 37 898 3074 Fragments arranged by size: 3074 939 898 552 288 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3815 3832 3985 4170 4615 325 Size: 1255 2235 17 153 185 445 1498 Fragments arranged by size: 2235 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2529 4034 5746 Size: 274 80 33 51 1443 552 1505 1712 Cuts at: 5746 96 Size: 138 Fragments arranged by size: 1712 1505 1443 552 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2058 2090 5748 5780 2058 Size: 32 3658 32 2066 Fragments arranged by size: 3658 2066 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5788 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3596 276 Size: 53 83 186 936 2062 2468 Fragments arranged by size: 2468 2062 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI