################################## # NO CUT ENZYMES ( 155 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I CpoI DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 487 GTATAC GT^[AC][GT]AC AcsI 133 GAATTT [GA]^AATT[CT] AfaI 525 GTAC GT^AC AlwNI 367 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 133 GAATTT [GA]^AATT[CT] BamHI 554 GGATCC G^GATCC BanI 757 GGCACC G^G[CT][GA]CC BcnI 753 CCGGG CC^[GC]GG BglII 228 AGATCT A^GATCT BmyI 756 GGGCAC G[AGT]GC[ACT]^C BseRI 650 CTCCTC GAGGAG(10/8) BsiEI 108 CGATCG CG[GA][CT]^CG Bsp1286I 756 GGGCAC G[AGT]GC[ACT]^C BspDI 106 ATCGAT AT^CGAT Bst1107I 487 GTATAC GTA^TAC BstF5I 750 CATCC GGATG(2/0) BstZ17I 487 GTATAC GTA^TAC ClaI 106 ATCGAT AT^CGAT DraI 136 TTTAAA TTT^AAA DrdI 880 GACAAAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 975 CCCGC CCCGC(4/6) FokI 750 CATCC GGATG(9/13) HaeIII 295 GGCC GG^CC HincII 582 GTTGAC GT[CT]^[GA]AC HindII 582 GTTGAC GT[CT]^[GA]AC MslI 340 CACTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 927 CAGCTG C[AC]G^C[GT]G NciI 753 CCGGG CC^[GC]GG PstI 232 CTGCAG CTGCA^G PvuI 108 CGATCG CGAT^CG PvuII 927 CAGCTG CAG^CTG RsaI 525 GTAC GT^AC SfcI 232 CTGCAG C^T[GA][CT]AG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 71 TCCGGA T^CCGGA 462 TCCGGA BbvI 234 GCAGC GCAGC(8/12) 722 GCAGC BfaI 226 CTAG C^TAG 304 CTAG BpmI 727 CTCCAG CTGGAG(16/14) 451 CTGGAG BsaWI 71 TCCGGA [AT]^CCGG[AT] 462 TCCGGA BseAI 71 TCCGGA T^CCGGA 462 TCCGGA BsgI 113 GTGCAG GTGCAG(16/14) 505 GTGCAG BspEI 71 TCCGGA T^CCGGA 462 TCCGGA DsaI 287 CCATGG C^C[GA][CT]GG 324 CCATGG EcoT14I 287 CCATGG C^C[AT][AT]GG 324 CCATGG MaeI 226 CTAG C^TAG 304 CTAG MaeIII 436 GTTAC ^GT[ACGT]AC 702 GTTAC MroI 71 TCCGGA T^CCGGA 462 TCCGGA MseI 137 TTAA T^TAA 764 TTAA NcoI 287 CCATGG C^CATGG 324 CCATGG NlaIV 554 GGATCC GG[ACGT]^[ACGT]CC 757 GGCACC PflMI 324 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 729 CCAGGACTTGG PleI 242 GACTC GAGTC(4/5) 676 GAGTC SfaNI 200 GCATC GCATC(5/9) 743 GCATC StyI 287 CCATGG C^C[AT][AT]GG 324 CCATGG TaqI 107 TCGA T^CGA 855 TCGA TfiI 534 GAATC G^A[AT]TC 630 GATTC Tru9I 137 TTAA T^TAA 764 TTAA TseI 234 GCAGC G^C[AT]GC 722 GCAGC TthHB8I 107 TCGA T^CGA 855 TCGA Van91I 324 CCATGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 729 CCAGGACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AciI AluI AlwI AvaII BbsI BpuAI BsaJI BsiYI BslI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI HapII HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII PpuMI Sau3AI Sau96I ScrFI Tsp509I TspRI XhoII