SIM2 ORF clone pFN21AE3524

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Halotag ORF Clone pFN21AE3524

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Clone info.

Species

human

Clone Name

pFN21AE3524 Sequence (with vector) Restriction Map

Product ID

FHC11667

ORF Sequence

DNA sequence (1710 bp)
Protein sequence (570 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3524

Symbol

SIM2

Alias : HMC13F06, HMC29C01, SIM, bHLHe15

Description

single-minded family bHLH transcription factor 2, transcript variant SIM2s

Original Clone ID

cp93524

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1710 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 570 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13646.1 1.9e-163 100.0 SIM2 CCDS5045.1 6.1e-102 69.2 SIM1 CCDS9754.1 3.4e-39 39.9 HIF1A CCDS9753.1 3.7e-39 39.9 HIF1A CCDS58324.1 3.8e-39 39.9 HIF1A The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001092 8 51 PF00010 Helix-loop-helix DNA-binding domain IPR013767 81 142 PF00989 PAS fold IPR013655 243 329 PF08447 PAS fold-3 IPR010578 358 529 PF06621 Single-minded HMMSmart IPR001092 6 58 SM00353 Helix-loop-helix DNA-binding domain IPR000014 79 145 SM00091 PAS IPR000014 220 286 SM00091 PAS IPR001610 292 335 SM00086 PAC motif ProfileScan IPR001092 1 54 PS50888 Helix-loop-helix DNA-binding domain IPR000014 77 149 PS50112 PAS IPR000014 233 288 PS50112 PAS IPR010578 336 570 PS51302 Single-minded
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_033664 570 600892 100.0 100.0 NP_005060 667 600892 100.0 92.1 XP_005267157 766 601665,603128 69.2 88.4 NP_005059 766 601665,603128 69.2 88.4 XP_016866686 766 601665,603128 69.2 88.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3524 Download

>KIEE3524  1710 bp
ATGAAGGAGAAGTCCAAGAATGCGGCCAAGACCAGGAGGGAGAAGGAAAATGGCGAGTTT
TACGAGCTTGCCAAGCTGCTCCCGCTGCCGTCGGCCATCACTTCGCAGCTGGACAAAGCG
TCCATCATCCGCCTCACCACGAGCTACCTGAAGATGCGCGCCGTCTTCCCCGAAGGTTTA
GGAGACGCGTGGGGACAGCCGAGCCGCGCCGGGCCCCTGGACGGCGTCGCCAAGGAGCTG
GGATCGCACTTGCTGCAGACTTTGGATGGATTTGTTTTTGTGGTAGCATCTGATGGCAAA
ATCATGTATATATCCGAGACCGCTTCTGTCCATTTAGGCTTATCCCAGGTGGAGCTCACG
GGCAACAGTATTTATGAATACATCCATCCTTCTGACCACGATGAGATGACCGCTGTCCTC
ACGGCCCACCAGCCGCTGCACCACCACCTGCTCCAAGAGTATGAGATAGAGAGGTCGTTC
TTTCTTCGAATGAAATGTGTCTTGGCGAAAAGGAACGCGGGCCTGACCTGCAGCGGATAC
AAGGTCATCCACTGCAGTGGCTACTTGAAGATCAGGCAGTATATGCTGGACATGTCCCTG
TACGACTCCTGCTACCAGATTGTGGGGCTGGTGGCCGTGGGCCAGTCGCTGCCACCCAGT
GCCATCACCGAGATCAAGCTGTACAGTAACATGTTCATGTTCAGGGCCAGCCTTGACCTG
AAGCTGATATTCCTGGATTCCAGGGTGACCGAGGTGACGGGGTACGAGCCGCAGGACCTG
ATCGAGAAGACCCTATACCATCACGTGCACGGCTGCGACGTGTTCCACCTCCGCTACGCA
CACCACCTCCTGTTGGTGAAGGGCCAGGTCACCACCAAGTACTACCGGCTGCTGTCCAAG
CGGGGCGGCTGGGTGTGGGTGCAGAGCTACGCCACCGTGGTGCACAACAGCCGCTCGTCC
CGGCCCCACTGCATCGTGAGTGTCAATTATGTACTCACGGAGATTGAATACAAGGAACTT
CAGCTGTCCCTGGAGCAGGTGTCCACTGCCAAGTCCCAGGACTCCTGGAGGACCGCCTTG
TCTACCTCACAAGAAACTAGGAAATTAGTGAAACCCAAAAATACCAAGATGAAGACAAAG
CTGAGAACAAACCCTTACCCCCCACAGCAATACAGCTCGTTCCAAATGGACAAACTGGAA
TGCGGCCAGCTCGGAAACTGGAGAGCCAGTCCCCCTGCAAGCGCTGCTGCTCCTCCAGAA
CTGCAGCCCCACTCAGAAAGCAGTGACCTTCTGTACACGCCATCCTACAGCCTGCCCTTC
TCCTACCATTACGGACACTTCCCTCTGGACTCTCACGTCTTCAGCAGCAAAAAGCCAATG
TTGCCGGCCAAGTTCGGGCAGCCCCAAGGATCCCCTTGTGAGGTGGCACGCTTTTTCCTG
AGCACACTGCCAGCCAGCGGTGAATGCCAGTGGCATTATGCCAACCCCCTAGTGCCTAGC
AGCTCGTCTCCAGCTAAAAATCCTCCAGAGCCACCGGCGAACACTGCTAGGCACAGCCTG
GTGCCAAGCTACGAAGGTGGGTCAGGTCTGCTCGTGGGGAAGGTGGGAGGACTGCGCACG
GCCGGGAGCCGAAGCAGCCATGGCGGTGGGTGGCAGATGGAGACAGAACCCTCACGCTTT
GGGCAAACTTGCCCTCTTTCTGCTTCTAAG

ORF Protein

Cloned ORF protein sequence for pFN21AE3524 Download

>KIEE3524  570 aa
MKEKSKNAAKTRREKENGEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRAVFPEGL
GDAWGQPSRAGPLDGVAKELGSHLLQTLDGFVFVVASDGKIMYISETASVHLGLSQVELT
GNSIYEYIHPSDHDEMTAVLTAHQPLHHHLLQEYEIERSFFLRMKCVLAKRNAGLTCSGY
KVIHCSGYLKIRQYMLDMSLYDSCYQIVGLVAVGQSLPPSAITEIKLYSNMFMFRASLDL
KLIFLDSRVTEVTGYEPQDLIEKTLYHHVHGCDVFHLRYAHHLLLVKGQVTTKYYRLLSK
RGGWVWVQSYATVVHNSRSSRPHCIVSVNYVLTEIEYKELQLSLEQVSTAKSQDSWRTAL
STSQETRKLVKPKNTKMKTKLRTNPYPPQQYSSFQMDKLECGQLGNWRASPPASAAAPPE
LQPHSESSDLLYTPSYSLPFSYHYGHFPLDSHVFSSKKPMLPAKFGQPQGSPCEVARFFL
STLPASGECQWHYANPLVPSSSSPAKNPPEPPANTARHSLVPSYEGGSGLLVGKVGGLRT
AGSRSSHGGGWQMETEPSRFGQTCPLSASK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3524 Download

>pFN21AE3524  6415 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAAGGAGAAGTCCAAGAATGCGGCCAAGACCAG
GAGGGAGAAGGAAAATGGCGAGTTTTACGAGCTTGCCAAGCTGCTCCCGCTGCCGTCGGC
CATCACTTCGCAGCTGGACAAAGCGTCCATCATCCGCCTCACCACGAGCTACCTGAAGAT
GCGCGCCGTCTTCCCCGAAGGTTTAGGAGACGCGTGGGGACAGCCGAGCCGCGCCGGGCC
CCTGGACGGCGTCGCCAAGGAGCTGGGATCGCACTTGCTGCAGACTTTGGATGGATTTGT
TTTTGTGGTAGCATCTGATGGCAAAATCATGTATATATCCGAGACCGCTTCTGTCCATTT
AGGCTTATCCCAGGTGGAGCTCACGGGCAACAGTATTTATGAATACATCCATCCTTCTGA
CCACGATGAGATGACCGCTGTCCTCACGGCCCACCAGCCGCTGCACCACCACCTGCTCCA
AGAGTATGAGATAGAGAGGTCGTTCTTTCTTCGAATGAAATGTGTCTTGGCGAAAAGGAA
CGCGGGCCTGACCTGCAGCGGATACAAGGTCATCCACTGCAGTGGCTACTTGAAGATCAG
GCAGTATATGCTGGACATGTCCCTGTACGACTCCTGCTACCAGATTGTGGGGCTGGTGGC
CGTGGGCCAGTCGCTGCCACCCAGTGCCATCACCGAGATCAAGCTGTACAGTAACATGTT
CATGTTCAGGGCCAGCCTTGACCTGAAGCTGATATTCCTGGATTCCAGGGTGACCGAGGT
GACGGGGTACGAGCCGCAGGACCTGATCGAGAAGACCCTATACCATCACGTGCACGGCTG
CGACGTGTTCCACCTCCGCTACGCACACCACCTCCTGTTGGTGAAGGGCCAGGTCACCAC
CAAGTACTACCGGCTGCTGTCCAAGCGGGGCGGCTGGGTGTGGGTGCAGAGCTACGCCAC
CGTGGTGCACAACAGCCGCTCGTCCCGGCCCCACTGCATCGTGAGTGTCAATTATGTACT
CACGGAGATTGAATACAAGGAACTTCAGCTGTCCCTGGAGCAGGTGTCCACTGCCAAGTC
CCAGGACTCCTGGAGGACCGCCTTGTCTACCTCACAAGAAACTAGGAAATTAGTGAAACC
CAAAAATACCAAGATGAAGACAAAGCTGAGAACAAACCCTTACCCCCCACAGCAATACAG
CTCGTTCCAAATGGACAAACTGGAATGCGGCCAGCTCGGAAACTGGAGAGCCAGTCCCCC
TGCAAGCGCTGCTGCTCCTCCAGAACTGCAGCCCCACTCAGAAAGCAGTGACCTTCTGTA
CACGCCATCCTACAGCCTGCCCTTCTCCTACCATTACGGACACTTCCCTCTGGACTCTCA
CGTCTTCAGCAGCAAAAAGCCAATGTTGCCGGCCAAGTTCGGGCAGCCCCAAGGATCCCC
TTGTGAGGTGGCACGCTTTTTCCTGAGCACACTGCCAGCCAGCGGTGAATGCCAGTGGCA
TTATGCCAACCCCCTAGTGCCTAGCAGCTCGTCTCCAGCTAAAAATCCTCCAGAGCCACC
GGCGAACACTGCTAGGCACAGCCTGGTGCCAAGCTACGAAGGTGGGTCAGGTCTGCTCGT
GGGGAAGGTGGGAGGACTGCGCACGGCCGGGAGCCGAAGCAGCCATGGCGGTGGGTGGCA
GATGGAGACAGAACCCTCACGCTTTGGGCAAACTTGCCCTCTTTCTGCTTCTAAGGTTTA
AACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTG
ATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAAT
AACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAG
TTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGAT
GCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAAC
CTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTT
GTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCT
TTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACC
CCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCC
TGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTC
GCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTG
GTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGAT
CTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGC
ACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAA
CTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAA
AAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGT
GATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCT
TTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT
GAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTG
CGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGG
ATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTT
ATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGG
CCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATG
GATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTC
GAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGT
TATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGG
CCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACG
AGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGAT
ACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTA
CCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCT
GTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCC
CCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAA
GACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATG
TAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAG
TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTT
GATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTA
CGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTC
AGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCA
CCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGA
TGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTAC
CCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTT
TCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTA
GCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATC
GAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT