(Circular) (Six-base) MAPSORT of: pFN21AE3524.seq Check: 6081 from: 1 to: 6415 pFN21AE3524 6415 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2460 3031 2460 Size: 571 5844 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4225 278 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 3758 1937 Size: 1149 672 4594 Fragments arranged by size: 4594 1149 672 Acc65I G'GTAC_C Cuts at: 3736 3736 Size: 6415 AclI AA'CG_TT Cuts at: 1563 4542 4915 1563 Size: 2979 373 3063 Fragments arranged by size: 3063 2979 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2174 2744 3008 3349 4478 5862 6130 1632 Size: 542 570 264 341 1129 1384 268 1917 Fragments arranged by size: 1917 1384 1129 570 542 341 268 264 AfeI AGC'GCT Cuts at: 3247 3247 Size: 6415 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6218 AflIII A'CryG_T Cuts at: 1203 2190 2595 2694 2823 5314 6291 1203 Size: 987 405 99 129 2491 977 1327 Fragments arranged by size: 2491 1327 987 977 405 129 99 AgeI A'CCGG_T Cuts at: 5246 5246 Size: 6415 AhdI GACnn_n'nnGTC Cuts at: 2122 5144 2122 Size: 3022 3393 AleI CACnn'nnGTG Cuts at: 1166 2559 2942 1166 Size: 1393 383 4639 Fragments arranged by size: 4639 1393 383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6415 AlwNI CAG_nnn'CTG Cuts at: 2440 2653 2782 4158 5730 2440 Size: 213 129 1376 1572 3125 Fragments arranged by size: 3125 1572 1376 213 129 ApaI G_GGCC'C Cuts at: 2220 2220 Size: 6415 ApaLI G'TGCA_C Cuts at: 2810 2945 4472 5628 2810 Size: 135 1527 1156 3597 Fragments arranged by size: 3597 1527 1156 135 ApoI r'AATT_y Cuts at: 1493 3724 3950 4102 6211 1493 Size: 2231 226 152 2109 1697 Fragments arranged by size: 2231 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4969 160 Size: 872 3937 1606 Fragments arranged by size: 3937 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 AvaI C'yCGr_G Cuts at: 1870 1943 3740 1870 Size: 73 1797 4545 Fragments arranged by size: 4545 1797 73 BamHI G'GATC_C Cuts at: 3413 3745 4112 3413 Size: 332 367 5716 Fragments arranged by size: 5716 367 332 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3565 3736 5191 618 Size: 325 217 627 1778 171 1455 1842 Fragments arranged by size: 1842 1778 1455 627 325 217 171 BanII G_rGCy'C Cuts at: 1391 2220 2361 3734 1391 Size: 829 141 1373 4072 Fragments arranged by size: 4072 1373 829 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2161 2798 3143 3355 928 Size: 971 262 637 345 212 3988 Fragments arranged by size: 3988 971 637 345 262 212 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4606 4640 1903 Size: 34 2669 34 3678 Fragments arranged by size: 3678 2669 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2534 4307 5517 2534 Size: 1773 1210 3432 Fragments arranged by size: 3432 1773 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6415 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5026 136 Size: 107 122 71 631 605 3354 1525 Fragments arranged by size: 3354 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6410 6410 Size: 6415 BlpI GC'TnA_GC Cuts at: 3832 3832 Size: 6415 Bme1580I G_kGCm'C Cuts at: 1790 2220 2814 2949 4476 5632 1790 Size: 430 594 135 1527 1156 2573 Fragments arranged by size: 2573 1527 1156 594 430 135 BmgBI CAC'GTC Cuts at: 1318 2824 3361 1318 Size: 1506 537 4372 Fragments arranged by size: 4372 1506 537 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2655 4146 5104 Size: 416 53 83 399 24 1238 1491 958 Cuts at: 5104 442 Size: 1753 Fragments arranged by size: 1753 1491 1238 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6415 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3056 3091 3243 3244 3498 3513 5075 1239 Size: 1817 35 152 1 254 15 1562 2579 Fragments arranged by size: 2579 1817 1562 254 152 35 15 1 Bpu10I CC'TnA_GC Cuts at: 3443 3443 Size: 6415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4540 5405 5703 4540 Size: 865 298 5252 Fragments arranged by size: 5252 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 5078 882 Size: 617 816 2763 2219 Fragments arranged by size: 2763 2219 816 617 BsaAI yAC'GTr Cuts at: 493 1204 2809 4206 493 Size: 711 1605 1397 2702 Fragments arranged by size: 2702 1605 1397 711 BsaBI GATnn'nnATC Cuts at: 6409 6409 Size: 6415 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 4222 4604 5239 Size: 53 83 186 936 696 1993 382 635 Cuts at: 5239 275 Size: 1451 Fragments arranged by size: 1993 1451 936 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4848 5246 5520 5667 6078 4848 Size: 398 274 147 411 5185 Fragments arranged by size: 5185 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2835 2865 3029 3059 3217 Size: 30 165 30 1432 30 164 30 158 Cuts at: 3217 3247 1178 Size: 30 4346 Fragments arranged by size: 4346 1432 165 164 158 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3246 1186 Size: 2060 4355 BseYI C'CCAG_C Cuts at: 2242 2913 5618 6114 2242 Size: 671 2705 496 2543 Fragments arranged by size: 2705 2543 671 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2426 2945 1163 Size: 573 690 519 4633 Fragments arranged by size: 4633 690 573 519 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3627 3865 4626 5654 1540 Size: 275 186 1626 238 761 1028 2301 Fragments arranged by size: 2301 1626 1028 761 275 238 186 BsiHKAI G_wGCw'C Cuts at: 2361 2814 2949 3449 4476 4561 5632 2361 Size: 453 135 500 1027 85 1071 3144 Fragments arranged by size: 3144 1071 1027 500 453 135 85 BsmI GAATG_Cn' Cuts at: 2029 3209 3473 3926 4019 2029 Size: 1180 264 453 93 4425 Fragments arranged by size: 4425 1180 453 264 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2181 3516 2181 Size: 1335 5080 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2361 2814 2949 3449 3734 4476 Size: 399 430 141 453 135 500 285 742 Cuts at: 4476 4561 5632 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 453 430 399 285 141 135 85 BspEI T'CCGG_A Cuts at: 6078 6078 Size: 6415 BspHI T'CATG_A Cuts at: 4304 6034 4304 Size: 1730 4685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2460 2540 3031 3770 844 Size: 1616 80 491 739 3489 Fragments arranged by size: 3489 1616 739 491 80 BsrBI CCG'CTC Cuts at: 1394 1823 2958 4302 1394 Size: 429 1135 1344 3507 Fragments arranged by size: 3507 1344 1135 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4910 5084 65 Size: 1183 3662 174 1396 Fragments arranged by size: 3662 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2889 3388 3538 5059 5246 1700 Size: 110 1079 499 150 1521 187 2869 Fragments arranged by size: 2869 1521 1079 499 187 150 110 BsrGI T'GTAC_A Cuts at: 96 2685 3297 96 Size: 2589 612 3214 Fragments arranged by size: 3214 2589 612 BssHII G'CGCG_C Cuts at: 1925 2161 1925 Size: 236 6179 BssSI C'ACGA_G Cuts at: 2143 3596 4475 5487 2143 Size: 1453 879 1012 3071 Fragments arranged by size: 3071 1453 1012 879 BstAPI GCAn_nnn'nTGC Cuts at: 794 2257 2587 3248 794 Size: 1463 330 661 3961 Fragments arranged by size: 3961 1463 661 330 BstBI TT'CG_AA Cuts at: 2491 5219 2491 Size: 2728 3687 BstEII G'GTnAC_C Cuts at: 1184 2749 2872 1184 Size: 1565 123 4727 Fragments arranged by size: 4727 1565 123 BstXI CCAn_nnnn'nTGG Cuts at: 2242 2561 3650 2242 Size: 319 1089 5007 Fragments arranged by size: 5007 1089 319 BstYI r'GATC_y Cuts at: 1972 3413 3745 4112 4497 4514 5966 6052 6064 Size: 1441 332 367 385 17 1452 86 12 Cuts at: 6064 6410 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1441 385 367 346 332 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2640 2940 3643 513 Size: 552 1575 300 703 3285 Fragments arranged by size: 3285 1575 703 552 300 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5676 Fragments arranged by size: 5676 683 56 BtsI GCAGTG_nn' Cuts at: 2554 2566 2971 3048 3292 3449 3546 3931 4725 Size: 12 405 77 244 157 97 385 794 Cuts at: 4725 4745 2554 Size: 20 4224 Fragments arranged by size: 4224 794 405 385 244 157 97 77 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6415 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6380 DraI TTT'AAA Cuts at: 3719 3719 Size: 6415 DraIII CAC_nnn'GTG Cuts at: 2663 2663 Size: 6415 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3082 3359 5422 809 Size: 507 1766 277 2063 1802 Fragments arranged by size: 2063 1802 1766 507 277 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2097 2637 Size: 54 1425 50 275 18 198 69 540 Cuts at: 2637 3208 3390 3624 3862 4751 6380 8 Size: 571 182 234 238 889 1629 43 Fragments arranged by size: 1629 1425 889 571 540 275 238 234 198 182 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3624 3862 1537 Size: 275 1812 238 4090 Fragments arranged by size: 4090 1812 275 238 EarI CTCTTCn'nnn_ Cuts at: 1353 4345 1353 Size: 2992 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 5002 5376 5522 387 Size: 1064 138 534 2879 374 146 1280 Fragments arranged by size: 2879 1280 1064 534 374 146 138 EcoICRI GAG'CTC Cuts at: 2359 2359 Size: 6415 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2744 3008 3056 3091 3243 3244 Size: 393 542 570 264 48 35 152 1 Cuts at: 3244 3349 3498 3513 4478 5075 5862 6130 1239 Size: 105 149 15 965 597 787 268 1524 Fragments arranged by size: 1524 965 787 597 570 542 393 268 264 152 149 105 48 35 15 1 EcoNI CCTnn'n_nnAGG Cuts at: 3038 3038 Size: 6415 EcoO109I rG'GnC_Cy Cuts at: 2217 2779 2217 Size: 562 5853 EcoRI G'AATT_C Cuts at: 3724 4102 3724 Size: 378 6037 FspI TGC'GCA Cuts at: 3620 4921 3620 Size: 1301 5114 HaeII r_GCGC'y Cuts at: 1678 3249 5562 1678 Size: 1571 2313 2531 Fragments arranged by size: 2531 2313 1571 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2231 2263 2532 Size: 32 135 32 536 32 91 32 269 Cuts at: 2532 2564 3029 3061 3217 3249 5056 5088 5130 Size: 32 465 32 156 32 1807 32 42 Cuts at: 5130 5162 1373 Size: 32 2626 Fragments arranged by size: 2626 1807 536 465 269 156 135 91 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3759 4005 669 Size: 988 281 1821 246 3079 Fragments arranged by size: 3079 1821 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6415 HpaI GTT'AAC Cuts at: 4005 4005 Size: 6415 KpnI G_GTAC'C Cuts at: 3740 3740 Size: 6415 MfeI C'AATT_G Cuts at: 4014 4014 Size: 6415 MluI A'CGCG_T Cuts at: 2190 6291 2190 Size: 4101 2314 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5529 5713 1305 Size: 4224 184 2007 Fragments arranged by size: 4224 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4593 Fragments arranged by size: 4593 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 2699 2942 3648 4373 4732 4891 Size: 648 1393 140 243 706 725 359 159 Cuts at: 4891 518 Size: 2042 Fragments arranged by size: 2042 1393 725 706 648 359 243 159 140 MspA1I CmG'CkG Cuts at: 1606 2089 2113 2417 2440 2538 3028 3462 3831 Size: 483 24 304 23 98 490 434 369 Cuts at: 3831 4508 5656 5901 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 490 483 434 369 304 245 98 24 23 NaeI GCC'GGC Cuts at: 3390 3390 Size: 6415 NcoI C'CATG_G Cuts at: 513 1065 3643 513 Size: 552 2578 3285 Fragments arranged by size: 3285 2578 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6415 NgoMIV G'CCGG_C Cuts at: 3388 3388 Size: 6415 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6415 NotI GC'GGCC_GC Cuts at: 3862 3862 Size: 6415 NspI r_CATG'y Cuts at: 1132 1229 2599 2698 3773 5318 1132 Size: 97 1370 99 1075 1545 2229 Fragments arranged by size: 2229 1545 1370 1075 99 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6415 PciI A'CATG_T Cuts at: 2595 2694 5314 2595 Size: 99 2620 3696 Fragments arranged by size: 3696 2620 99 PflMI CCAn_nnn'nTGG Cuts at: 1108 2626 1108 Size: 1518 4897 PfoI T'CCnGG_A Cuts at: 2736 3068 2736 Size: 332 6083 PmeI GTTT'AAAC Cuts at: 3719 3719 Size: 6415 PmlI CAC'GTG Cuts at: 2809 2809 Size: 6415 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2465 2497 4484 4516 5997 6029 2465 Size: 32 1987 32 1481 32 2851 Fragments arranged by size: 2851 1987 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2779 2779 Size: 6415 PshAI GACnn'nnGTC Cuts at: 1613 2417 1613 Size: 804 5611 PsiI TTA'TAA Cuts at: 3985 3985 Size: 6415 PspOMI G'GGCC_C Cuts at: 2216 2216 Size: 6415 PstI C_TGCA'G Cuts at: 830 2262 2537 2561 3270 3767 830 Size: 1432 275 24 709 497 3478 Fragments arranged by size: 3478 1432 709 497 275 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6415 PvuII CAG'CTG Cuts at: 2113 3028 2113 Size: 915 5500 SacI G_AGCT'C Cuts at: 2361 2361 Size: 6415 SalI G'TCGA_C Cuts at: 1936 3757 1936 Size: 1821 4594 SbfI CC_TGCA'GG Cuts at: 3767 3767 Size: 6415 ScaI AGT'ACT Cuts at: 2885 4663 6375 2885 Size: 1778 1712 2925 Fragments arranged by size: 2925 1778 1712 SfcI C'TryA_G Cuts at: 826 1045 2258 2533 2557 3266 3310 3763 4898 Size: 219 1213 275 24 709 44 453 1135 Cuts at: 4898 5579 5770 826 Size: 681 191 1471 Fragments arranged by size: 1471 1213 1135 709 681 453 275 219 191 44 24 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6415 SgrAI Cr'CCGG_yG Cuts at: 1700 3538 1700 Size: 1838 4577 SmaI CCC'GGG Cuts at: 1872 3742 1872 Size: 1870 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4519 5420 5682 820 Size: 197 926 2576 901 262 1553 Fragments arranged by size: 2576 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4206 493 Size: 3713 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6415 SphI G_CATG'C Cuts at: 1132 3773 1132 Size: 2641 3774 SspI AAT'ATT Cuts at: 5 52 4339 5 Size: 47 4287 2081 Fragments arranged by size: 4287 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6415 StyI C'CwwG_G Cuts at: 513 1065 2235 3409 3643 3854 513 Size: 552 1170 1174 234 211 3074 Fragments arranged by size: 3074 1174 1170 552 234 211 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2674 2768 2905 2911 3642 4442 4459 Size: 1255 1094 94 137 6 731 800 17 Cuts at: 4459 4612 4797 5242 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1094 800 731 445 185 153 137 94 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2685 2883 2995 Size: 274 80 33 51 1443 708 198 112 Cuts at: 2995 3297 4661 6373 96 Size: 302 1364 1712 138 Fragments arranged by size: 1712 1443 1364 708 302 274 198 138 112 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2835 2867 3423 3455 6375 6407 2835 Size: 32 556 32 2920 32 2843 Fragments arranged by size: 2920 2843 556 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2228 1316 Size: 912 5503 XbaI T'CTAG_A Cuts at: 3751 3751 Size: 6415 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6415 XmaI C'CCGG_G Cuts at: 1870 3740 1870 Size: 1870 4545 XmnI GAAnn'nnTTC Cuts at: 4544 4544 Size: 6415 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4223 276 Size: 53 83 186 936 2689 2468 Fragments arranged by size: 2689 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI