(Linear) (Six-base) MAPSORT of: KIEE3524.seq Check: 7666 from: 1 to: 1710 KIEE3524.orf With 182 enzymes: SgfI * July 3, 2012 18:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 455 1026 1710 Size: 455 571 684 Fragments arranged by size: 684 571 455 AccI GT'mk_AC Cuts at: 0 1081 1710 Size: 1081 629 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 739 1003 1344 1710 Size: 169 570 264 341 366 Fragments arranged by size: 570 366 341 264 169 AfeI AGC'GCT Cuts at: 0 1242 1710 Size: 1242 468 AflIII A'CryG_T Cuts at: 0 185 590 689 818 1710 Size: 185 405 99 129 892 Fragments arranged by size: 892 405 185 129 99 AhdI GACnn_n'nnGTC Cuts at: 0 117 1710 Size: 117 1593 AleI CACnn'nnGTG Cuts at: 0 554 937 1710 Size: 554 383 773 Fragments arranged by size: 773 554 383 AlwNI CAG_nnn'CTG Cuts at: 0 435 648 777 1710 Size: 435 213 129 933 Fragments arranged by size: 933 435 213 129 ApaI G_GGCC'C Cuts at: 0 215 1710 Size: 215 1495 ApaLI G'TGCA_C Cuts at: 0 805 940 1710 Size: 805 135 770 Fragments arranged by size: 805 770 135 BamHI G'GATC_C Cuts at: 0 1408 1710 Size: 1408 302 BanI G'GyrC_C Cuts at: 0 1560 1710 Size: 1560 150 BanII G_rGCy'C Cuts at: 0 215 356 1710 Size: 215 141 1354 Fragments arranged by size: 1354 215 141 BbsI GAAGACnn'nnnn_ Cuts at: 0 156 793 1138 1350 1710 Size: 156 637 345 212 360 Fragments arranged by size: 637 360 345 212 156 BciVI GTATCCnnnnn_n' Cuts at: 0 529 1710 Size: 529 1181 Bme1580I G_kGCm'C Cuts at: 0 215 809 944 1710 Size: 215 594 135 766 Fragments arranged by size: 766 594 215 135 BmgBI CAC'GTC Cuts at: 0 819 1356 1710 Size: 819 537 354 Fragments arranged by size: 819 537 354 BmrI ACTGGGnnnn_n' Cuts at: 0 650 1710 Size: 650 1060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1051 1086 1238 1239 1493 1508 1710 Size: 1051 35 152 1 254 15 202 Fragments arranged by size: 1051 254 202 152 35 15 1 Bpu10I CC'TnA_GC Cuts at: 0 1438 1710 Size: 1438 272 BsaI GGTCTCn'nnnn_ Cuts at: 0 310 1710 Size: 310 1400 BsaAI yAC'GTr Cuts at: 0 804 1710 Size: 804 906 BsaHI Gr'CG_yC Cuts at: 0 224 1710 Size: 224 1486 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 830 860 1024 1054 1212 1242 1710 Size: 830 30 164 30 158 30 468 Fragments arranged by size: 830 468 164 158 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1241 1710 Size: 1241 469 BseYI C'CCAG_C Cuts at: 0 237 908 1710 Size: 237 671 802 Fragments arranged by size: 802 671 237 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 940 1710 Size: 421 519 770 Fragments arranged by size: 770 519 421 BsiEI CG_ry'CG Cuts at: 0 1622 1710 Size: 1622 88 BsiHKAI G_wGCw'C Cuts at: 0 356 809 944 1444 1710 Size: 356 453 135 500 266 Fragments arranged by size: 500 453 356 266 135 BsmI GAATG_Cn' Cuts at: 0 24 1204 1468 1710 Size: 24 1180 264 242 Fragments arranged by size: 1180 264 242 24 BsmBI CGTCTCn'nnnn_ Cuts at: 0 176 1511 1710 Size: 176 1335 199 Fragments arranged by size: 1335 199 176 Bsp1286I G_dGCh'C Cuts at: 0 215 356 809 944 1444 1710 Size: 215 141 453 135 500 266 Fragments arranged by size: 500 453 266 215 141 135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 455 535 1026 1710 Size: 455 80 491 684 Fragments arranged by size: 684 491 455 80 BsrBI CCG'CTC Cuts at: 0 953 1710 Size: 953 757 BsrFI r'CCGG_y Cuts at: 0 884 1383 1533 1710 Size: 884 499 150 177 Fragments arranged by size: 884 499 177 150 BsrGI T'GTAC_A Cuts at: 0 680 1292 1710 Size: 680 612 418 Fragments arranged by size: 680 612 418 BssHII G'CGCG_C Cuts at: 0 156 1710 Size: 156 1554 BssSI C'ACGA_G Cuts at: 0 138 1591 1710 Size: 138 1453 119 Fragments arranged by size: 1453 138 119 BstAPI GCAn_nnn'nTGC Cuts at: 0 252 582 1243 1710 Size: 252 330 661 467 Fragments arranged by size: 661 467 330 252 BstBI TT'CG_AA Cuts at: 0 486 1710 Size: 486 1224 BstEII G'GTnAC_C Cuts at: 0 744 867 1710 Size: 744 123 843 Fragments arranged by size: 843 744 123 BstXI CCAn_nnnn'nTGG Cuts at: 0 237 556 1645 1710 Size: 237 319 1089 65 Fragments arranged by size: 1089 319 237 65 BstYI r'GATC_y Cuts at: 0 1408 1710 Size: 1408 302 BtgI C'CryG_G Cuts at: 0 635 935 1638 1710 Size: 635 300 703 72 Fragments arranged by size: 703 635 300 72 BtsI GCAGTG_nn' Cuts at: 0 549 561 966 1043 1287 1444 1541 1710 Size: 549 12 405 77 244 157 97 169 Fragments arranged by size: 549 405 244 169 157 97 77 12 DraIII CAC_nnn'GTG Cuts at: 0 658 1710 Size: 658 1052 DrdI GACnn_nn'nnGTC Cuts at: 0 1077 1354 1710 Size: 1077 277 356 Fragments arranged by size: 1077 356 277 EaeI y'GGCC_r Cuts at: 0 23 92 632 1203 1385 1619 1710 Size: 23 69 540 571 182 234 91 Fragments arranged by size: 571 540 234 182 91 69 23 EagI C'GGCC_G Cuts at: 0 1619 1710 Size: 1619 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 118 1710 Size: 118 1592 EcoICRI GAG'CTC Cuts at: 0 354 1710 Size: 354 1356 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 739 1003 1051 1086 1238 1239 1344 Size: 169 570 264 48 35 152 1 105 Cuts at: 1344 1493 1508 1710 Size: 149 15 202 Fragments arranged by size: 570 264 202 169 152 149 105 48 35 15 1 EcoNI CCTnn'n_nnAGG Cuts at: 0 1033 1710 Size: 1033 677 EcoO109I rG'GnC_Cy Cuts at: 0 212 774 1710 Size: 212 562 936 Fragments arranged by size: 936 562 212 FspI TGC'GCA Cuts at: 0 1615 1710 Size: 1615 95 HaeII r_GCGC'y Cuts at: 0 1244 1710 Size: 1244 466 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 226 258 527 559 1024 1056 Size: 103 32 91 32 269 32 465 32 Cuts at: 1056 1212 1244 1710 Size: 156 32 466 Fragments arranged by size: 466 465 269 156 103 91 32 32 32 32 32 MluI A'CGCG_T Cuts at: 0 185 1710 Size: 185 1525 MslI CAynn'nnrTG Cuts at: 0 554 694 937 1643 1710 Size: 554 140 243 706 67 Fragments arranged by size: 706 554 243 140 67 MspA1I CmG'CkG Cuts at: 0 84 108 412 435 533 1023 1457 1710 Size: 84 24 304 23 98 490 434 253 Fragments arranged by size: 490 434 304 253 98 84 24 23 NaeI GCC'GGC Cuts at: 0 1385 1710 Size: 1385 325 NcoI C'CATG_G Cuts at: 0 1638 1710 Size: 1638 72 NgoMIV G'CCGG_C Cuts at: 0 1383 1710 Size: 1383 327 NspI r_CATG'y Cuts at: 0 594 693 1710 Size: 594 99 1017 Fragments arranged by size: 1017 594 99 PciI A'CATG_T Cuts at: 0 590 689 1710 Size: 590 99 1021 Fragments arranged by size: 1021 590 99 PflMI CCAn_nnn'nTGG Cuts at: 0 621 1710 Size: 621 1089 PfoI T'CCnGG_A Cuts at: 0 731 1063 1710 Size: 731 332 647 Fragments arranged by size: 731 647 332 PmlI CAC'GTG Cuts at: 0 804 1710 Size: 804 906 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 460 492 1710 Size: 460 32 1218 Fragments arranged by size: 1218 460 32 PpuMI rG'GwC_Cy Cuts at: 0 774 1710 Size: 774 936 PshAI GACnn'nnGTC Cuts at: 0 412 1710 Size: 412 1298 PspOMI G'GGCC_C Cuts at: 0 211 1710 Size: 211 1499 PstI C_TGCA'G Cuts at: 0 257 532 556 1265 1710 Size: 257 275 24 709 445 Fragments arranged by size: 709 445 275 257 24 PvuII CAG'CTG Cuts at: 0 108 1023 1710 Size: 108 915 687 Fragments arranged by size: 915 687 108 SacI G_AGCT'C Cuts at: 0 356 1710 Size: 356 1354 ScaI AGT'ACT Cuts at: 0 880 1710 Size: 880 830 SfcI C'TryA_G Cuts at: 0 253 528 552 1261 1305 1710 Size: 253 275 24 709 44 405 Fragments arranged by size: 709 405 275 253 44 24 SgrAI Cr'CCGG_yG Cuts at: 0 1533 1710 Size: 1533 177 StyI C'CwwG_G Cuts at: 0 230 1404 1638 1710 Size: 230 1174 234 72 Fragments arranged by size: 1174 234 230 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 669 763 900 906 1637 1710 Size: 669 94 137 6 731 73 Fragments arranged by size: 731 669 137 94 73 6 TatI w'GTAC_w Cuts at: 0 680 878 990 1292 1710 Size: 680 198 112 302 418 Fragments arranged by size: 680 418 302 198 112 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 830 862 1418 1450 1710 Size: 830 32 556 32 260 Fragments arranged by size: 830 556 260 32 32 Tth111I GACn'n_nGTC Cuts at: 0 223 1710 Size: 223 1487 Enzymes that do cut: AarI AccI AcuI AfeI AflIII AhdI AleI AlwNI ApaI ApaLI BamHI BanI BanII BbsI BciVI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I MluI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI ScaI SfcI SgrAI StyI TaqII TatI TstI Tth111I Enzymes that do not cut: AatII Acc65I AclI AflII AgeI AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmtI BplI BpuEI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EarI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MmeI MscI NarI NdeI NheI NotI NruI NsiI PacI PasI PmeI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XcmI XhoI XmaI XmnI ZraI