DCAF4 ORF clone pFN21AE3218

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3218

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Clone info.

Species

human

Clone Name

pFN21AE3218 Sequence (with vector) Restriction Map

Product ID

FHC25224

Flexi Clone PID

FXC25224

ORF Sequence

DNA sequence (1485 bp)
Protein sequence (495 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3218

Symbol

DCAF4

Alias : WDR21, WDR21A

Description

DDB1 and CUL4 associated factor 4, transcript variant 1

Original Clone ID

cp93218

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1485 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 495 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9809.1 2.5e-180 99.4 DCAF4 CCDS55926.1 4.1e-176 98.0 DCAF4 CCDS9810.1 7.4e-141 99.2 DCAF4 CCDS41968.2 1.2e-112 86.9 DCAF4 CCDS33978.1 2.3e-108 76.5 DCAF4L1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR019781 368 398 PF00400 WD40 repeat HMMSmart IPR001680 360 398 SM00320 WD40 repeat IPR001680 401 442 SM00320 WD40 repeat ProfileScan IPR017986 366 451 PS50294 WD40-repeat-containing domain IPR019782 369 407 PS50082 WD40 repeat 2
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056419 495 616372 99.4 100.0 XP_016876697 495 616372 99.4 100.0 XP_016876696 495 616372 99.4 100.0 XP_016876691 496 616372 99.2 100.0 XP_016876695 496 616372 99.2 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3218 Download

>KIEE3218  1485 bp
ATGAATAAAAGTCGCTGGCAGAGTAGAAGACGACATGGGAGAAGAAGCCACCAGCAGAAC
CCTTGGTTCAGACTCCGTGATTCTGAAGACAGGTCTGACTCCCGGGCAGCACAGCCCGCT
CACGATTCCGGCCACGGTGATGACGAGTCTCCGTCAACCTCGTCTGGCACAGCTGGGACC
TCCTCTGTGCCAGAGCTACCTGGGTTTTACTTTGACCCTGAAAAGAAACGCTACTTCCGC
TTGCTCCCTGGACATAACAACTGCAACCCCCTGACGAAAGAGAGCATCCGGCAGAAGGAG
ATGGAGAGCAAGAGACTGCGGCTGCTCCAGGAAGAAGACAGACGGAAAAAGATTGCCAGG
ATGGGATTTAATGCATCTTCCATGCTACGAAAAAGCCAGCTGGGTTTTCTCAACGTCACC
AATTACTGCCATTTAGCCCACGAGCTGCGTCTCAGCTGCATGGAGAGGAAAAAGGTCCAG
ATTCGAAGCATGGATCCCTCCGCCTTGGCAAGCGACCGATTTAACCTCATACTGGCAGAT
ACCAACAGTGACCGGCTCTTCACAGTGAACGATGTTACAGTTGGAGGCTCCAAGTATGGT
ATCATCAACCTGCAAAGTCTGAAGACCCCTACGCTCAAGGTGTTCATGCACGAAAACCTC
TACTTCACCAACCGGAAGGTGAATTCGGTGTGCTGGGCCTCGCTGAATCACTTGGATTCC
CACATTCTGCTATGCCTCATGGGACTCGCAGAGACTCCAGGCTGTGCCACCCTGCTCCCA
GCATCACTGTTCGTCAATAGTCACCCAGGAATAGACCGGCCTGGCATGCTCTGCAGTTTC
CGGATCCCTGGTGCCTGGTCCTGTGCCTGGTCCCTGAATATCCAAGCAAATAACTGCTTC
AGTACAGGCTTGTCTCGGCGGGTCCTGTTGACCAACGTGGTGACGGGACACCGGCAGTCC
TTTGGGACCAACAGTGATGTCTTGGCCCAGCAGTTTGCTTTCATGGCTCCTCTGCTGTTT
AATGGCTGCCGCTCTGGGGAAATCTTTGCCATTGATCTGCGTTGTGGAAATCAAGGCAAG
GGATGGAAGGCCACCCGCCTGTTTCATGATTCAGCAGTGACCTCTGTGCGGATCCTCCAA
GATGAGCAATACCTGATGGCTTCAGACATGGCTGGAAAGATCAAGCTGTGGGACCTGAGG
ACCACGAAGTGCGTAAGGCAGTACGAAGGCCACGTGAATGAGTACGCCTACCTGCCCCTG
CATGTGCACGAGGAAGAAGGAATCCTGGTGGCAGTGGGCCAGGACTGCTACACGATAATC
TGGAGCCTCCACGATGCCCGCCTACTGAGAACCATACCCTCCCCGTACCCTGCCTCCAAG
GCCGACATTCCCAGTGTGGCCTTCTCGTCGCGGCTGGGGGGCTCCCGGGGCGCGCCGGGG
CTGCTCATGGCTGTCGGGCAGGACCTTTACTGTTACTCCTACAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE3218 Download

>KIEE3218  495 aa
MNKSRWQSRRRHGRRSHQQNPWFRLRDSEDRSDSRAAQPAHDSGHGDDESPSTSSGTAGT
SSVPELPGFYFDPEKKRYFRLLPGHNNCNPLTKESIRQKEMESKRLRLLQEEDRRKKIAR
MGFNASSMLRKSQLGFLNVTNYCHLAHELRLSCMERKKVQIRSMDPSALASDRFNLILAD
TNSDRLFTVNDVTVGGSKYGIINLQSLKTPTLKVFMHENLYFTNRKVNSVCWASLNHLDS
HILLCLMGLAETPGCATLLPASLFVNSHPGIDRPGMLCSFRIPGAWSCAWSLNIQANNCF
STGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFAFMAPLLFNGCRSGEIFAIDLRCGNQGK
GWKATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVNEYAYLPL
HVHEEEGILVAVGQDCYTIIWSLHDARLLRTIPSPYPASKADIPSVAFSSRLGGSRGAPG
LLMAVGQDLYCYSYS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3218 Download

>pFN21AE3218  6190 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATAAAAGTCGCTGGCAGAGTAGAAGACGACA
TGGGAGAAGAAGCCACCAGCAGAACCCTTGGTTCAGACTCCGTGATTCTGAAGACAGGTC
TGACTCCCGGGCAGCACAGCCCGCTCACGATTCCGGCCACGGTGATGACGAGTCTCCGTC
AACCTCGTCTGGCACAGCTGGGACCTCCTCTGTGCCAGAGCTACCTGGGTTTTACTTTGA
CCCTGAAAAGAAACGCTACTTCCGCTTGCTCCCTGGACATAACAACTGCAACCCCCTGAC
GAAAGAGAGCATCCGGCAGAAGGAGATGGAGAGCAAGAGACTGCGGCTGCTCCAGGAAGA
AGACAGACGGAAAAAGATTGCCAGGATGGGATTTAATGCATCTTCCATGCTACGAAAAAG
CCAGCTGGGTTTTCTCAACGTCACCAATTACTGCCATTTAGCCCACGAGCTGCGTCTCAG
CTGCATGGAGAGGAAAAAGGTCCAGATTCGAAGCATGGATCCCTCCGCCTTGGCAAGCGA
CCGATTTAACCTCATACTGGCAGATACCAACAGTGACCGGCTCTTCACAGTGAACGATGT
TACAGTTGGAGGCTCCAAGTATGGTATCATCAACCTGCAAAGTCTGAAGACCCCTACGCT
CAAGGTGTTCATGCACGAAAACCTCTACTTCACCAACCGGAAGGTGAATTCGGTGTGCTG
GGCCTCGCTGAATCACTTGGATTCCCACATTCTGCTATGCCTCATGGGACTCGCAGAGAC
TCCAGGCTGTGCCACCCTGCTCCCAGCATCACTGTTCGTCAATAGTCACCCAGGAATAGA
CCGGCCTGGCATGCTCTGCAGTTTCCGGATCCCTGGTGCCTGGTCCTGTGCCTGGTCCCT
GAATATCCAAGCAAATAACTGCTTCAGTACAGGCTTGTCTCGGCGGGTCCTGTTGACCAA
CGTGGTGACGGGACACCGGCAGTCCTTTGGGACCAACAGTGATGTCTTGGCCCAGCAGTT
TGCTTTCATGGCTCCTCTGCTGTTTAATGGCTGCCGCTCTGGGGAAATCTTTGCCATTGA
TCTGCGTTGTGGAAATCAAGGCAAGGGATGGAAGGCCACCCGCCTGTTTCATGATTCAGC
AGTGACCTCTGTGCGGATCCTCCAAGATGAGCAATACCTGATGGCTTCAGACATGGCTGG
AAAGATCAAGCTGTGGGACCTGAGGACCACGAAGTGCGTAAGGCAGTACGAAGGCCACGT
GAATGAGTACGCCTACCTGCCCCTGCATGTGCACGAGGAAGAAGGAATCCTGGTGGCAGT
GGGCCAGGACTGCTACACGATAATCTGGAGCCTCCACGATGCCCGCCTACTGAGAACCAT
ACCCTCCCCGTACCCTGCCTCCAAGGCCGACATTCCCAGTGTGGCCTTCTCGTCGCGGCT
GGGGGGCTCCCGGGGCGCGCCGGGGCTGCTCATGGCTGTCGGGCAGGACCTTTACTGTTA
CTCCTACAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTG
CAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG
CCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATA
AGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATT
TGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTT
AACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTT
TTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCG
CTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGT
TTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGA
AATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTC
ATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATT
CAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCT
CACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGT
TACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGT
TTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGAC
GCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTAC
TCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCT
GCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCG
AAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGG
GAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCA
ATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAA
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTT
CCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATC
ATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGG
AGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATT
AAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAA
AGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAA
AAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGA
CAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTC
CGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTT
CTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCT
GTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTA
GCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT
ACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAA
GAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTT
GCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTAT
CAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGAT
GGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGG
TAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGC
GATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGC
GCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCT
GCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGG
ATCGAGATCT