Restriction map for
pFN21AE3218
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AflIII AgeI AhdI AlwNI
Aor51HI ApaI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaI
BsgI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI Bst1107I BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV FbaI FseI FspI HaeII
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccIII 839 TCCGGA T^CCGGA
AcsI 681 GAATTC [GA]^AATT[CT]
Alw44I 1264 GTGCAC G^TGCAC
AocI 1194 CCTGAGG CC^T[ACGT]AGG
ApaLI 1264 GTGCAC G^TGCAC
ApoI 681 GAATTC [GA]^AATT[CT]
AscI 1429 GGCGCGCC GG^CGCGCC
AspHI 1264 GTGCAC G[AT]GC[AT]^C
BanI 850 GGTGCC G^G[CT][GA]CC
BanII 1419 GGGCTC G[GA]GC[CT]^C
BbrPI 1231 CACGTG CAC^GTG
BglI 1372 GCCTCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1231 CACGTG [CT]AC^GT[GA]
BseAI 839 TCCGGA T^CCGGA
BsiEI 513 CGACCG CG[GA][CT]^CG
BsiHKAI 1264 GTGCAC G[AT]GC[AT]^C
BsmBI 448 CGTCTC CGTCTC(1/5)
BspEI 839 TCCGGA T^CCGGA
BspHI 1104 TCATGA T^CATGA
BssHII 1430 GCGCGC G^CGCGC
BstAPI 824 GCATGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 482 TTCGAA TT^CGAA
Bsu36I 1194 CCTGAGG CC^T[ACGT]AGG
DraIII 1203 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 132 CCACGG C^C[GA][CT]GG
EaeI 129 CGGCCA [CT]^GGCC[GA]
EarI 556 CTCTTC CTCTTC(1/4)
Eco81I 1194 CCTGAGG CC^T[ACGT]AGG
EcoRI 681 GAATTC G^AATTC
EcoT22I 371 ATGCAT ATGCA^T
HgaI 447 GCGTC GACGC(5/10)
Ksp632I 556 CTCTTC CTCTTC(1/4)
MroI 839 TCCGGA T^CCGGA
NsiI 371 ATGCAT ATGCA^T
NspV 482 TTCGAA TT^CGAA
PmaCI 1231 CACGTG CAC^GTG
PmlI 1231 CACGTG CAC^GTG
PstI 831 CTGCAG CTGCA^G
RcaI 1104 TCATGA T^CATGA
SapI 555 GCTCTTC GCTCTTC(1/4)
SfuI 482 TTCGAA TT^CGAA
SmlI 634 CTCAAG C^T[CT][GA]AG
SphI 824 GCATGC GCATG^C
TaqI 483 TCGA T^CGA
TthHB8I 483 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1409 CGCG CG^CG
1431 CGCG
AvaI 101 CCCGGG C^[CT]CG[GA]G
1424 CCCGGG
BmyI 1264 GTGCAC G[AGT]GC[ACT]^C
1419 GGGCTC
BsaWI 671 ACCGGA [AT]^CCGG[AT]
839 TCCGGA
BseRI 180 CTCCTC GAGGAG(10/8)
1007 CTCCTC
BsoBI 101 CCCGGG C^[CT]CG[GA]G
1424 CCCGGG
Bsp1286I 1264 GTGCAC G[AGT]GC[ACT]^C
1419 GGGCTC
BspMI 608 ACCTGC ACCTGC(4/8)
1250 ACCTGC
BsrBI 116 CCGCTC CCGCTC(-3/-3)
1029 CCGCTC
BsrI 531 ACTGG ACTGG(1/-1)
1391 CCAGT
BssSI 439 CACGAG CACGAG(-5/-1)
1267 CACGAG
BstUI 1409 CGCG CG^CG
1431 CGCG
CfoI 1430 GCGC GCG^C
1432 GCGC
HhaI 1430 GCGC GCG^C
1432 GCGC
HinP1I 1430 GCGC G^CGC
1432 GCGC
HincII 153 GTCAAC GT[CT]^[GA]AC
927 GTTGAC
HindII 153 GTCAAC GT[CT]^[GA]AC
927 GTTGAC
MslI 133 CACGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1231 CACGTGAATG
MvnI 1409 CGCG CG^CG
1431 CGCG
NspI 824 GCATGC [GA]CATG^[CT]
1260 GCATGT
SfcI 831 CTGCAG C^T[GA][CT]AG
1479 CTACAG
SmaI 101 CCCGGG CCC^GGG
1424 CCCGGG
Tsp509I 421 AATT ^AATT
682 AATT
XmaCI 101 CCCGGG C^CCGGG
1424 CCCGGG
XmaI 101 CCCGGG C^CCGGG
1424 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BbsI BbvI BcnI BpmI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsrFI BssKI BstF5I BstNI
BstYI Cac8I Cfr10I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp45I TspRI XhoII