(Circular) (Six-base) MAPSORT of: pFN21AE3218.seq Check: 9789 from: 1 to: 6190 pFN21AE3218 6190 bp With 182 enzymes: SgfI * July 6, 2012 17:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4000 278 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3533 1937 Size: 1596 4594 Acc65I G'GTAC_C Cuts at: 3511 3511 Size: 6190 AclI AA'CG_TT Cuts at: 1563 4317 4690 1563 Size: 2754 373 3063 Fragments arranged by size: 3063 2754 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 2645 2887 3150 4253 5637 5905 1632 Size: 477 536 242 263 1103 1384 268 1917 Fragments arranged by size: 1917 1384 1103 536 477 268 263 242 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5993 AflIII A'CryG_T Cuts at: 1203 5089 6066 1203 Size: 3886 977 1327 Fragments arranged by size: 3886 1327 977 AgeI A'CCGG_T Cuts at: 5021 5021 Size: 6190 AhdI GACnn_n'nnGTC Cuts at: 4919 4919 Size: 6190 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6190 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2336 2914 1184 Size: 1152 578 4460 Fragments arranged by size: 4460 1152 578 AlwNI CAG_nnn'CTG Cuts at: 3933 5505 3933 Size: 1572 4618 ApaLI G'TGCA_C Cuts at: 3269 4247 5403 3269 Size: 978 1156 4056 Fragments arranged by size: 4056 1156 978 ApoI r'AATT_y Cuts at: 1493 2686 3499 3725 3877 5986 1493 Size: 1193 813 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 813 226 152 AscI GG'CGCG_CC Cuts at: 3435 3435 Size: 6190 AseI AT'TA_AT Cuts at: 160 1032 4744 160 Size: 872 3712 1606 Fragments arranged by size: 3712 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 AvaI C'yCGr_G Cuts at: 1870 1943 2106 3429 3515 1870 Size: 73 163 1323 86 4545 Fragments arranged by size: 4545 1323 163 86 73 BamHI G'GATC_C Cuts at: 2497 2847 3135 3520 3887 2497 Size: 350 288 385 367 4800 Fragments arranged by size: 4800 385 367 350 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2855 3511 4966 618 Size: 325 217 627 1068 656 1455 1842 Fragments arranged by size: 1842 1455 1068 656 627 325 217 BanII G_rGCy'C Cuts at: 1391 3428 3509 1391 Size: 2037 81 4072 Fragments arranged by size: 4072 2037 81 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2038 2097 2346 2633 928 Size: 971 139 59 249 287 4485 Fragments arranged by size: 4485 971 287 249 139 59 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4381 4415 1903 Size: 34 2444 34 3678 Fragments arranged by size: 3678 2444 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4082 5292 4082 Size: 1210 4980 BclI T'GATC_A Cuts at: 1548 1548 Size: 6190 BfrBI ATG'CAT Cuts at: 2378 2378 Size: 6190 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3383 4801 136 Size: 107 122 71 631 605 1711 1418 1525 Fragments arranged by size: 1711 1525 1418 631 605 122 107 71 BglII A'GATC_T Cuts at: 6185 6185 Size: 6190 BlpI GC'TnA_GC Cuts at: 3607 3607 Size: 6190 Bme1580I G_kGCm'C Cuts at: 1790 3273 4251 5407 1790 Size: 1483 978 1156 2573 Fragments arranged by size: 2573 1483 1156 978 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6190 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 3921 4879 Size: 416 53 83 399 24 1973 531 958 Cuts at: 4879 442 Size: 1753 Fragments arranged by size: 1973 1753 958 531 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6190 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2315 2745 3346 4850 1239 Size: 1076 430 601 1504 2579 Fragments arranged by size: 2579 1504 1076 601 430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2624 4315 5180 5478 2624 Size: 1691 865 298 3336 Fragments arranged by size: 3336 1691 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4853 882 Size: 617 3354 2219 Fragments arranged by size: 3354 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3238 3981 493 Size: 711 2034 743 2702 Fragments arranged by size: 2702 2034 743 711 BsaBI GATnn'nnATC Cuts at: 6184 6184 Size: 6190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3997 4379 5014 275 Size: 53 83 186 936 2464 382 635 1451 Fragments arranged by size: 2464 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2676 2844 4623 5021 5295 5442 5853 2676 Size: 168 1779 398 274 147 411 3013 Fragments arranged by size: 3013 1779 411 398 274 168 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3362 3392 3464 3494 1178 Size: 30 165 30 1959 30 72 30 3874 Fragments arranged by size: 3874 1959 165 72 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2176 3003 1186 Size: 990 827 4373 Fragments arranged by size: 4373 990 827 BseYI C'CCAG_C Cuts at: 2177 2404 2697 2782 2991 3418 5393 5889 2177 Size: 227 293 85 209 427 1975 496 2478 Fragments arranged by size: 2478 1975 496 427 293 227 209 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5617 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2521 3640 4401 5429 1540 Size: 275 186 520 1119 761 1028 2301 Fragments arranged by size: 2301 1119 1028 761 520 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 4251 4336 5407 3273 Size: 978 85 1071 4056 Fragments arranged by size: 4056 1071 978 85 BsmI GAATG_Cn' Cuts at: 3701 3794 3701 Size: 93 6097 BsmBI CGTCTCn'nnnn_ Cuts at: 2459 2459 Size: 6190 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3273 3428 3509 4251 4336 5407 1391 Size: 399 1483 155 81 742 85 1071 2174 Fragments arranged by size: 2174 1483 1071 742 399 155 85 81 BspEI T'CCGG_A Cuts at: 2844 5853 2844 Size: 3009 3181 BspHI T'CATG_A Cuts at: 3109 4079 5809 3109 Size: 970 1730 3490 Fragments arranged by size: 3490 1730 970 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2622 3264 3545 844 Size: 1778 642 281 3489 Fragments arranged by size: 3489 1778 642 281 BsrBI CCG'CTC Cuts at: 1394 1823 2123 3036 4077 1394 Size: 429 300 913 1041 3507 Fragments arranged by size: 3507 1041 913 429 300 BsrDI GCAATG_nn' Cuts at: 65 1248 4685 4859 65 Size: 1183 3437 174 1396 Fragments arranged by size: 3437 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 2820 2955 4834 5021 1700 Size: 110 746 264 135 1879 187 2869 Fragments arranged by size: 2869 1879 746 264 187 135 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6190 BssHII G'CGCG_C Cuts at: 1925 3435 1925 Size: 1510 4680 BssSI C'ACGA_G Cuts at: 2444 3272 4250 5262 2444 Size: 828 978 1012 3372 Fragments arranged by size: 3372 1012 978 828 BstAPI GCAn_nnn'nTGC Cuts at: 794 2835 794 Size: 2041 4149 BstBI TT'CG_AA Cuts at: 2488 4994 2488 Size: 2506 3684 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6190 BstYI r'GATC_y Cuts at: 1972 2497 2847 3135 3520 3887 4272 4289 5741 Size: 525 350 288 385 367 385 17 1452 Cuts at: 5741 5827 5839 6185 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 525 385 385 367 350 346 288 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3200 3200 Size: 6190 BtgI C'CryG_G Cuts at: 513 1065 2137 513 Size: 552 1072 4566 Fragments arranged by size: 4566 1072 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5451 Fragments arranged by size: 5451 683 56 BtsI GCAGTG_nn' Cuts at: 3126 3303 3706 4500 4520 3126 Size: 177 403 794 20 4796 Fragments arranged by size: 4796 794 403 177 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6190 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6155 DraI TTT'AAA Cuts at: 3494 3494 Size: 6190 DraIII CAC_nnn'GTG Cuts at: 3213 3213 Size: 6190 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5197 809 Size: 507 3881 1802 Fragments arranged by size: 3881 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 3637 4526 Size: 54 1425 50 275 18 304 1503 889 Cuts at: 4526 6155 8 Size: 1629 43 Fragments arranged by size: 1629 1503 1425 889 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3637 1537 Size: 275 1825 4090 Fragments arranged by size: 4090 1825 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2567 4120 1353 Size: 1214 1553 3423 Fragments arranged by size: 3423 1553 1214 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2494 4777 5151 5297 387 Size: 1064 138 905 2283 374 146 1280 Fragments arranged by size: 2283 1280 1064 905 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2315 2645 2745 2887 3150 3346 Size: 393 477 206 330 100 142 263 196 Cuts at: 3346 4253 4850 5637 5905 1239 Size: 907 597 787 268 1524 Fragments arranged by size: 1524 907 787 597 477 393 330 268 263 206 196 142 100 EcoO109I rG'GnC_Cy Cuts at: 2181 2926 3196 3466 2181 Size: 745 270 270 4905 Fragments arranged by size: 4905 745 270 270 EcoRI G'AATT_C Cuts at: 2686 3499 3877 2686 Size: 813 378 4999 Fragments arranged by size: 4999 813 378 FspI TGC'GCA Cuts at: 4696 4696 Size: 6190 HaeII r_GCGC'y Cuts at: 1678 5337 1678 Size: 3659 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2141 2167 2173 2514 Size: 32 135 32 563 6 26 6 341 Cuts at: 2514 2546 4831 4863 4905 4937 1373 Size: 32 2285 32 42 32 2626 Fragments arranged by size: 2626 2285 563 341 135 42 32 32 32 32 32 26 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 2160 2934 3534 3780 669 Size: 988 281 222 774 600 246 3079 Fragments arranged by size: 3079 988 774 600 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 6190 HpaI GTT'AAC Cuts at: 3780 3780 Size: 6190 KpnI G_GTAC'C Cuts at: 3515 3515 Size: 6190 MfeI C'AATT_G Cuts at: 3789 3789 Size: 6190 MluI A'CGCG_T Cuts at: 6066 6066 Size: 6190 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2566 5304 5488 1305 Size: 1261 2738 184 2007 Fragments arranged by size: 2738 2007 1261 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4368 Fragments arranged by size: 4368 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 3240 4148 4507 4666 518 Size: 648 976 1098 908 359 159 2042 Fragments arranged by size: 2042 1098 976 908 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2177 2404 2460 3606 4283 5431 5676 1606 Size: 571 227 56 1146 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 677 571 245 227 56 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5638 NdeI CA'TA_TG Cuts at: 387 387 Size: 6190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6190 NotI GC'GGCC_GC Cuts at: 3637 3637 Size: 6190 NsiI A_TGCA'T Cuts at: 2380 2380 Size: 6190 NspI r_CATG'y Cuts at: 1132 1229 2833 3269 3548 5093 1132 Size: 97 1604 436 279 1545 2229 Fragments arranged by size: 2229 1604 1545 436 279 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6190 PciI A'CATG_T Cuts at: 5089 5089 Size: 6190 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6190 PfoI T'CCnGG_A Cuts at: 2331 2331 Size: 6190 PmeI GTTT'AAAC Cuts at: 3494 3494 Size: 6190 PmlI CAC'GTG Cuts at: 3238 3238 Size: 6190 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4259 4291 5772 5804 4259 Size: 32 1481 32 4645 Fragments arranged by size: 4645 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2181 2926 3196 3466 2181 Size: 745 270 270 4905 Fragments arranged by size: 4905 745 270 270 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6190 PsiI TTA'TAA Cuts at: 3760 3760 Size: 6190 PstI C_TGCA'G Cuts at: 830 2840 3542 830 Size: 2010 702 3478 Fragments arranged by size: 3478 2010 702 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6190 PvuII CAG'CTG Cuts at: 2177 2404 2460 2177 Size: 227 56 5907 Fragments arranged by size: 5907 227 56 SalI G'TCGA_C Cuts at: 1936 3532 1936 Size: 1596 4594 SapI GCTCTTCn'nnn_ Cuts at: 2567 2567 Size: 6190 SbfI CC_TGCA'GG Cuts at: 3542 3542 Size: 6190 ScaI AGT'ACT Cuts at: 4438 6150 4438 Size: 1712 4478 SfcI C'TryA_G Cuts at: 826 1045 2836 3484 3538 4673 5354 5545 826 Size: 219 1791 648 54 1135 681 191 1471 Fragments arranged by size: 1791 1471 1135 681 648 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6190 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6190 SmaI CCC'GGG Cuts at: 1872 2108 3431 3517 1872 Size: 236 1323 86 4545 Fragments arranged by size: 4545 1323 236 86 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 4294 5195 5457 820 Size: 197 926 696 1655 901 262 1553 Fragments arranged by size: 1655 1553 926 901 696 262 197 SnaBI TAC'GTA Cuts at: 493 3981 493 Size: 3488 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6190 SphI G_CATG'C Cuts at: 1132 2833 3548 1132 Size: 1701 715 3774 Fragments arranged by size: 3774 1701 715 SspI AAT'ATT Cuts at: 5 52 4114 5 Size: 47 4062 2081 Fragments arranged by size: 4062 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6190 StyI C'CwwG_G Cuts at: 513 1065 2066 2508 3381 3629 513 Size: 552 1001 442 873 248 3074 Fragments arranged by size: 3074 1001 873 552 442 248 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2535 2823 4217 4234 4387 4572 5017 Size: 1255 955 288 1394 17 153 185 445 Cuts at: 5017 325 Size: 1498 Fragments arranged by size: 1498 1394 1255 955 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2906 4436 6148 Size: 274 80 33 51 1443 929 1530 1712 Cuts at: 6148 96 Size: 138 Fragments arranged by size: 1712 1530 1443 929 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2705 2737 2839 2871 2945 2962 2977 2994 3276 Size: 32 102 32 74 17 15 17 282 Cuts at: 3276 3308 6150 6182 2705 Size: 32 2842 32 2713 Fragments arranged by size: 2842 2713 282 102 74 32 32 32 32 17 17 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6190 XbaI T'CTAG_A Cuts at: 3526 3526 Size: 6190 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6190 XmaI C'CCGG_G Cuts at: 1870 2106 3429 3515 1870 Size: 236 1323 86 4545 Fragments arranged by size: 4545 1323 236 86 XmnI GAAnn'nnTTC Cuts at: 4319 4319 Size: 6190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3998 276 Size: 53 83 186 936 2464 2468 Fragments arranged by size: 2468 2464 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI