WDSUB1 ORF clone pFN21AE3206

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3206

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Clone info.

Species

human

Clone Name

pFN21AE3206 Sequence (with vector) Restriction Map

Product ID

FHC11670

ORF Sequence

DNA sequence (1428 bp)
Protein sequence (476 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3206

Symbol

WDSUB1

Alias : UBOX6, WDSAM1

Description

WD repeat, sterile alpha motif and U-box domain containing 1, transcript variant 3

Original Clone ID

cp93206

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1428 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 476 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS2208.1 1.2e-129 100.0 WDSUB1 CCDS82525.1 1.4e-103 90.1 WDSUB1 CCDS77477.1 2.8e-61 80.7 WDSUB1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR020472 69 83 PR00320 G-protein beta WD-40 repeat IPR020472 112 126 PR00320 G-protein beta WD-40 repeat IPR020472 296 310 PR00320 G-protein beta WD-40 repeat HMMPfam IPR019781 3 38 PF00400 WD40 repeat IPR019781 50 82 PF00400 WD40 repeat IPR019781 98 125 PF00400 WD40 repeat IPR019781 144 167 PF00400 WD40 repeat IPR019781 171 217 PF00400 WD40 repeat IPR019781 230 267 PF00400 WD40 repeat IPR019781 273 308 PF00400 WD40 repeat IPR011510 332 394 PF07647 Sterile alpha motif IPR003613 404 476 PF04564 U box domain HMMSmart IPR001680 1 38 SM00320 WD40 repeat IPR001680 43 82 SM00320 WD40 repeat IPR001680 85 125 SM00320 WD40 repeat IPR001680 128 167 SM00320 WD40 repeat IPR001680 169 218 SM00320 WD40 repeat IPR001680 228 267 SM00320 WD40 repeat IPR001680 270 309 SM00320 WD40 repeat IPR001660 329 396 SM00454 Sterile alpha motif domain IPR003613 407 470 SM00504 U box domain ProfileScan IPR017986 8 318 PS50294 WD40-repeat-containing domain IPR019782 50 91 PS50082 WD40 repeat 2 IPR019782 93 134 PS50082 WD40 repeat 2 IPR019782 146 167 PS50082 WD40 repeat 2 IPR019782 235 276 PS50082 WD40 repeat 2 IPR019782 277 311 PS50082 WD40 repeat 2 IPR001660 332 396 PS50105 Sterile alpha motif domain Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3206 Download

>KIEE3206  1428 bp
ATGGTGAAACTGATTCACACATTAGCTGATCATGGTGACGATGTCAACTGCTGTGCCTTC
TCCTTTTCCCTCTTGGCTACTTGCTCCTTGGACAAAACAATTCGCCTGTACTCGTTACGT
GACTTTACTGAACTGCCACATTCTCCATTGAAGTTTCATACCTATGCTGTCCACTGCTGC
TGTTTCTCCCCTTCAGGACATATTTTGGCATCGTGTTCAACAGATGGTACCACTGTCCTA
TGGAATACTGAAAATGGACAGATGCTGGCAGTGATGGAACAGCCTAGTGGCAGCCCTGTG
AGGGTTTGCCAGTTTTCCCCAGACTCCACGTGTTTGGCATCAGGGGCAGCTGATGGAACT
GTGGTTTTGTGGAATGCACAGTCATACAAATTATATAGATGTGGTAGTGTTAAAGATGGC
TCCTTGGCGGCATGTGCATTTTCTCCTAATGGAAGCTTCTTTGTCACTGGCTCCTCATGT
GGTGATTTAACAGTGTGGGATGATAAAATGAGGTGTCTGCATAGTGAAAAAGCACATGAT
CTTGGAATTACCTGCTGCGATTTTTCTTCACAGCCAGTTTCTGATGGAGAACAAGGTCTT
CAGTTTTTTCGACTGGCATCATGTGGTCAGGATTGCCAAGTCAAAATTTGGATTGTTTCT
TTTACCCATATCTTAGGTTTTGAATTAAAATATAAAAGTACACTGAGTGGGCACTGTGCT
CCTGTTCTGGCTTGTGCTTTTTCCCATGATGGGCAGATGCTAGTCTCAGGGTCAGTGGAT
AAGTCTGTCATAGTATATGATACTAATACTGAGAATATACTTCACACATTGACTCAGCAC
ACCAGGTATGTCACAACTTGTGCTTTTGCACCTAATACCCTTTTACTTGCTACTGGTTCA
ATGGACAAAACAGTGAACATCTGGCAATTTGACCTGGAAACACTTTGCCAAGCAAGGCGC
ACAGAACATCAGCTGAAGCAATTTACCGAAGATTGGTCAGAGGAGGATGTCTCAACATGG
CTTTGTGCACAAGATTTAAAAGATCTTGTTGGTATTTTCAAGATGAATAACATTGATGGA
AAAGAACTGTTGAATCTTACAAAAGAAAGTCTGGCTGATGATTTGAAAATTGAATCTCTA
GGACTGCGTAGTAAAGTGCTGAGGAAAATTGAAGAGCTCAGGACCAAGGTTAAATCCCTT
TCTTCAGGAATTCCTGATGAATTTATATGTCCAATAACTAGAGAACTTATGAAAGATCCG
GTCATCGCATCAGATGGCTATTCATATGAAAAGGAAGCAATGGAAAATTGGATCAGCAAA
AAGAAACGTACAAGTCCCATGACAAATCTTGTTCTTCCTTCAGCGGTACTTACACCAAAT
AGGACTCTGAAAATGGCCATCAATAGATGGCTGGAGACACACCAAAAG

ORF Protein

Cloned ORF protein sequence for pFN21AE3206 Download

>KIEE3206  476 aa
MVKLIHTLADHGDDVNCCAFSFSLLATCSLDKTIRLYSLRDFTELPHSPLKFHTYAVHCC
CFSPSGHILASCSTDGTTVLWNTENGQMLAVMEQPSGSPVRVCQFSPDSTCLASGAADGT
VVLWNAQSYKLYRCGSVKDGSLAACAFSPNGSFFVTGSSCGDLTVWDDKMRCLHSEKAHD
LGITCCDFSSQPVSDGEQGLQFFRLASCGQDCQVKIWIVSFTHILGFELKYKSTLSGHCA
PVLACAFSHDGQMLVSGSVDKSVIVYDTNTENILHTLTQHTRYVTTCAFAPNTLLLATGS
MDKTVNIWQFDLETLCQARRTEHQLKQFTEDWSEEDVSTWLCAQDLKDLVGIFKMNNIDG
KELLNLTKESLADDLKIESLGLRSKVLRKIEELRTKVKSLSSGIPDEFICPITRELMKDP
VIASDGYSYEKEAMENWISKKKRTSPMTNLVLPSAVLTPNRTLKMAINRWLETHQK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3206 Download

>pFN21AE3206  6133 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGTGAAACTGATTCACACATTAGCTGATCATGG
TGACGATGTCAACTGCTGTGCCTTCTCCTTTTCCCTCTTGGCTACTTGCTCCTTGGACAA
AACAATTCGCCTGTACTCGTTACGTGACTTTACTGAACTGCCACATTCTCCATTGAAGTT
TCATACCTATGCTGTCCACTGCTGCTGTTTCTCCCCTTCAGGACATATTTTGGCATCGTG
TTCAACAGATGGTACCACTGTCCTATGGAATACTGAAAATGGACAGATGCTGGCAGTGAT
GGAACAGCCTAGTGGCAGCCCTGTGAGGGTTTGCCAGTTTTCCCCAGACTCCACGTGTTT
GGCATCAGGGGCAGCTGATGGAACTGTGGTTTTGTGGAATGCACAGTCATACAAATTATA
TAGATGTGGTAGTGTTAAAGATGGCTCCTTGGCGGCATGTGCATTTTCTCCTAATGGAAG
CTTCTTTGTCACTGGCTCCTCATGTGGTGATTTAACAGTGTGGGATGATAAAATGAGGTG
TCTGCATAGTGAAAAAGCACATGATCTTGGAATTACCTGCTGCGATTTTTCTTCACAGCC
AGTTTCTGATGGAGAACAAGGTCTTCAGTTTTTTCGACTGGCATCATGTGGTCAGGATTG
CCAAGTCAAAATTTGGATTGTTTCTTTTACCCATATCTTAGGTTTTGAATTAAAATATAA
AAGTACACTGAGTGGGCACTGTGCTCCTGTTCTGGCTTGTGCTTTTTCCCATGATGGGCA
GATGCTAGTCTCAGGGTCAGTGGATAAGTCTGTCATAGTATATGATACTAATACTGAGAA
TATACTTCACACATTGACTCAGCACACCAGGTATGTCACAACTTGTGCTTTTGCACCTAA
TACCCTTTTACTTGCTACTGGTTCAATGGACAAAACAGTGAACATCTGGCAATTTGACCT
GGAAACACTTTGCCAAGCAAGGCGCACAGAACATCAGCTGAAGCAATTTACCGAAGATTG
GTCAGAGGAGGATGTCTCAACATGGCTTTGTGCACAAGATTTAAAAGATCTTGTTGGTAT
TTTCAAGATGAATAACATTGATGGAAAAGAACTGTTGAATCTTACAAAAGAAAGTCTGGC
TGATGATTTGAAAATTGAATCTCTAGGACTGCGTAGTAAAGTGCTGAGGAAAATTGAAGA
GCTCAGGACCAAGGTTAAATCCCTTTCTTCAGGAATTCCTGATGAATTTATATGTCCAAT
AACTAGAGAACTTATGAAAGATCCGGTCATCGCATCAGATGGCTATTCATATGAAAAGGA
AGCAATGGAAAATTGGATCAGCAAAAAGAAACGTACAAGTCCCATGACAAATCTTGTTCT
TCCTTCAGCGGTACTTACACCAAATAGGACTCTGAAAATGGCCATCAATAGATGGCTGGA
GACACACCAAAAGGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT