################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BamHI BbeI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 226 GGTACC G^GTACC AflIII 328 ACGTGT A^C[GA][CT]GT Alw44I 1025 GTGCAC G^TGCAC ApaLI 1025 GTGCAC G^TGCAC Asp718I 226 GGTACC G^GTACC AspI 37 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1181 GGACC G^G[AT]CC BalI 1394 TGGCCA TGG^CCA BanI 226 GGTACC G^G[CT][GA]CC BanII 1174 GAGCTC G[GA]GC[CT]^C BbrPI 327 CACGTG CAC^GTG BbsI 596 GTCTTC GAAGAC(2/6) BbvCI 1158 GCTGAGG CCTCAGC(-5/-2) BclI 27 TGATCA T^GATCA BglII 1041 AGATCT A^GATCT BpmI 1411 CTGGAG CTGGAG(16/14) BpuAI 596 GTCTTC GAAGAC(2/6) BsaWI 1257 TCCGGT [AT]^CCGG[AT] BsmFI 1334 GTCCC GGGAC(10/14) BsmI 372 GAATGC GAATGC(1/-1) BspMI 550 ACCTGC ACCTGC(4/8) BsrDI 1297 GCAATG GCAATG(2/0) BstXI 326 CCACGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 264 GCTGGC GC[ACGT]^[ACGT]GC CfoI 957 GCGC GCG^C Cfr13I 1181 GGACC G^G[ACGT]CC DraI 1035 TTTAAA TTT^AAA DraIII 701 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1394 TGGCCA [CT]^GGCC[GA] EarI 1171 GAAGAG CTCTTC(1/4) EcoRI 1208 GAATTC G^AATTC FbaI 27 TGATCA T^GATCA HaeIII 1395 GGCC GG^CC HapII 1258 CCGG C^CGG HhaI 957 GCGC GCG^C HinP1I 957 GCGC G^CGC HincII 43 GTCAAC GT[CT]^[GA]AC HindII 43 GTCAAC GT[CT]^[GA]AC HindIII 453 AAGCTT A^AGCTT HpaII 1258 CCGG C^CGG KpnI 226 GGTACC GGTAC^C Ksp632I 1171 GAAGAG CTCTTC(1/4) MluNI 1394 TGGCCA TGG^CCA MscI 1394 TGGCCA TGG^CCA MslI 157 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 1258 CCGG C^CGG NdeI 1283 CATATG CA^TATG NspI 430 GCATGT [GA]CATG^[CT] PflFI 37 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 327 CACGTG CAC^GTG PmlI 327 CACGTG CAC^GTG SacI 1174 GAGCTC GAGCT^C SapI 1171 GAAGAGC GCTCTTC(1/4) Sau96I 1181 GGACC G^G[ACGT]CC SexAI 841 ACCAGGT A^CC[AT]GGT TaqI 609 TCGA T^CGA Tth111I 37 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 609 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 427 GCGG CCGC(-3/-1) 1363 GCGG AlwI 1255 GATCC GGATC(4/5) 1310 GGATC AlwNI 259 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 575 CAGTTTCTG BsaAI 116 TACGTG [CT]AC^GT[GA] 327 CACGTG BseRI 471 CTCCTC GAGGAG(10/8) 1000 GAGGAG BssKI 842 CCAGG ^CC[ACGT]GG 933 CCTGG BstF5I 498 GGATG GGATG(2/0) 1005 GGATG BstNI 842 CCAGG CC^[AT]GG 933 CCTGG BstYI 1041 AGATCT [GA]^GATC[CT] 1254 AGATCC EcoRII 842 CCAGG ^CC[AT]GG 933 CCTGG FokI 498 GGATG GGATG(9/13) 1005 GGATG MflI 1041 AGATCT [GA]^GATC[CT] 1254 AGATCC MvaI 842 CCAGG CC^[AT]GG 933 CCTGG MwoI 337 GCATCAGGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 427 GCGGCATGTGC PvuII 347 CAGCTG CAG^CTG 970 CAGCTG ScrFI 842 CCAGG CC^[ACGT]GG 933 CCTGG XhoII 1041 AGATCT [GA]^GATC[CT] 1254 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AfaI AluI ApoI AspHI BbvI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI DdeI DpnII EcoT14I Fnu4HI HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I NdeII NlaIII NlaIV PleI RsaI Sau3AI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI