(Circular) (Six-base) MAPSORT of: pFN21AE3206.seq Check: 2277 from: 1 to: 6133 pFN21AE3206 6133 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 2231 3454 2231 Size: 1223 4910 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2588 2999 3192 3348 4196 5580 5848 Size: 549 407 411 193 156 848 1384 268 Cuts at: 5848 1632 Size: 1917 Fragments arranged by size: 1917 1384 848 549 411 407 268 193 156 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 2333 5032 6009 1203 Size: 1130 2699 977 1327 Fragments arranged by size: 2699 1327 1130 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6133 AlwNI CAG_nnn'CTG Cuts at: 2269 2585 3876 5448 2269 Size: 316 1291 1572 2954 Fragments arranged by size: 2954 1572 1291 316 ApaLI G'TGCA_C Cuts at: 3030 4190 5346 3030 Size: 1160 1156 3817 Fragments arranged by size: 3817 1160 1156 ApoI r'AATT_y Cuts at: 1493 2649 3213 3224 3442 3668 3820 5929 1493 Size: 1156 564 11 218 226 152 2109 1697 Fragments arranged by size: 2109 1697 1156 564 226 218 152 11 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 3458 1870 Size: 73 1515 4545 Fragments arranged by size: 4545 1515 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2223 2256 2223 Size: 33 6100 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2231 3454 4909 618 Size: 325 217 627 444 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 444 325 217 BanII G_rGCy'C Cuts at: 1391 3183 3452 1391 Size: 1792 269 4072 Fragments arranged by size: 4072 1792 269 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2594 928 Size: 971 695 4467 Fragments arranged by size: 4467 971 695 BbvCI CC'TCA_GC Cuts at: 3164 3164 Size: 6133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4324 4358 1903 Size: 34 2387 34 3678 Fragments arranged by size: 3678 2387 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 2032 1548 Size: 484 5649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4744 136 Size: 107 122 71 631 605 3072 1525 Fragments arranged by size: 3072 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3046 6128 3046 Size: 3082 3051 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2718 3034 4194 5350 1790 Size: 928 316 1160 1156 2573 Fragments arranged by size: 2573 1160 1156 928 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4822 442 Size: 416 53 83 399 24 2447 958 1753 Fragments arranged by size: 2447 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3031 3031 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3437 4793 1239 Size: 2198 1356 2579 Fragments arranged by size: 2579 2198 1356 Bpu10I CC'TnA_GC Cuts at: 3164 3182 3164 Size: 18 6115 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4258 5123 5421 4258 Size: 865 298 4970 Fragments arranged by size: 4970 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4796 882 Size: 617 3297 2219 Fragments arranged by size: 3297 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2123 2334 3924 493 Size: 711 919 211 1590 2702 Fragments arranged by size: 2702 1590 919 711 211 BsaBI GATnn'nnATC Cuts at: 6127 6127 Size: 6133 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3940 4322 4957 275 Size: 53 83 186 936 2407 382 635 1451 Fragments arranged by size: 2407 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3262 4566 4964 5238 5385 5796 3262 Size: 1304 398 274 147 411 3599 Fragments arranged by size: 3599 1304 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5908 Fragments arranged by size: 5908 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2467 3020 1186 Size: 1281 553 4299 Fragments arranged by size: 4299 1281 553 BseYI C'CCAG_C Cuts at: 5336 5832 5336 Size: 496 5637 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3583 4344 5372 1540 Size: 275 186 1582 761 1028 2301 Fragments arranged by size: 2301 1582 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2725 3034 3183 4194 4279 5350 2725 Size: 309 149 1011 85 1071 3508 Fragments arranged by size: 3508 1071 1011 309 149 85 BsmI GAATG_Cn' Cuts at: 2383 3644 3737 2383 Size: 1261 93 4779 Fragments arranged by size: 4779 1261 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2718 2725 3034 3183 3452 4194 4279 Size: 399 928 7 309 149 269 742 85 Cuts at: 4279 5350 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 928 742 399 309 269 149 85 7 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2564 3488 844 Size: 1720 924 3489 Fragments arranged by size: 3489 1720 924 BsrBI CCG'CTC Cuts at: 1394 1823 4020 1394 Size: 429 2197 3507 Fragments arranged by size: 3507 2197 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3309 4628 4802 65 Size: 1183 2061 1319 174 1396 Fragments arranged by size: 2061 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4777 4964 1700 Size: 110 2967 187 2869 Fragments arranged by size: 2967 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 4193 5205 4193 Size: 1012 5121 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstXI CCAn_nnnn'nTGG Cuts at: 2338 2338 Size: 6133 BstYI r'GATC_y Cuts at: 1972 3046 3259 3463 3830 4215 4232 5684 5770 Size: 1074 213 204 367 385 17 1452 86 Cuts at: 5770 5782 6128 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1074 385 367 346 213 204 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4642 Fragments arranged by size: 4642 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3253 487 Size: 683 56 2027 3367 Fragments arranged by size: 3367 2027 683 56 BtsI GCAGTG_nn' Cuts at: 2176 2280 3649 4443 4463 2176 Size: 104 1369 794 20 3846 Fragments arranged by size: 3846 1369 794 104 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3042 3437 3042 Size: 395 5738 DraIII CAC_nnn'GTG Cuts at: 2711 2711 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3399 3580 4469 Size: 54 1425 50 275 18 1569 181 889 Cuts at: 4469 6098 8 Size: 1629 43 Fragments arranged by size: 1629 1569 1425 889 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3580 1537 Size: 275 1768 4090 Fragments arranged by size: 4090 1768 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3171 4063 1353 Size: 1818 892 3423 Fragments arranged by size: 3423 1818 892 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3181 3181 Size: 6133 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2588 2999 3192 3348 3437 4196 Size: 393 549 407 411 193 156 89 759 Cuts at: 4196 4793 5580 5848 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 759 597 549 411 407 393 268 193 156 89 EcoRI G'AATT_C Cuts at: 3213 3442 3820 3213 Size: 229 378 5526 Fragments arranged by size: 5526 378 229 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 5280 1678 Size: 3602 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373 Size: 32 135 32 3202 32 42 32 2626 Fragments arranged by size: 3202 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2050 3477 3723 669 Size: 988 281 112 1427 246 3079 Fragments arranged by size: 3079 1427 988 281 246 112 HindIII A'AGCT_T Cuts at: 748 2458 748 Size: 1710 4423 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KpnI G_GTAC'C Cuts at: 2235 3458 2235 Size: 1223 4910 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5247 5431 1305 Size: 3942 184 2007 Fragments arranged by size: 3942 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3401 10 Size: 54 1425 343 1569 2742 Fragments arranged by size: 2742 1569 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2166 4091 4450 4609 518 Size: 648 1000 1925 359 159 2042 Fragments arranged by size: 2042 1925 1000 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2354 2977 3368 3549 4226 5374 5619 1606 Size: 748 623 391 181 677 1148 245 2120 Fragments arranged by size: 2120 1148 748 677 623 391 245 181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4642 Fragments arranged by size: 4642 939 552 NdeI CA'TA_TG Cuts at: 387 3289 387 Size: 2902 3231 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2439 3491 5036 1132 Size: 97 1210 1052 1545 2229 Fragments arranged by size: 2229 1545 1210 1052 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PmlI CAC'GTG Cuts at: 2334 2334 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PstI C_TGCA'G Cuts at: 830 3485 830 Size: 2655 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2354 2977 2354 Size: 623 5510 SacI G_AGCT'C Cuts at: 3183 3183 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SapI GCTCTTCn'nnn_ Cuts at: 3171 3171 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SexAI A'CCwGG_T Cuts at: 2846 2846 Size: 6133 SfcI C'TryA_G Cuts at: 826 1045 3481 4616 5297 5488 826 Size: 219 2436 1135 681 191 1471 Fragments arranged by size: 2436 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6133 SmaI CCC'GGG Cuts at: 1872 3460 1872 Size: 1588 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4237 5138 5400 820 Size: 197 926 2294 901 262 1553 Fragments arranged by size: 2294 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 3491 1132 Size: 2359 3774 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6133 StyI C'CwwG_G Cuts at: 513 1065 2004 2091 2427 3189 3572 513 Size: 552 939 87 336 762 383 3074 Fragments arranged by size: 3074 939 762 552 383 336 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4160 4177 4330 4515 4960 325 Size: 1255 2580 17 153 185 445 1498 Fragments arranged by size: 2580 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 2702 4379 Size: 274 80 33 51 1443 135 590 1677 Cuts at: 4379 6091 96 Size: 1712 138 Fragments arranged by size: 1712 1677 1443 590 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 2045 1316 Size: 729 5404 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6133 XmaI C'CCGG_G Cuts at: 1870 3458 1870 Size: 1588 4545 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI