KCNK2 ORF clone pFN21AE3186

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE3186

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Clone info.

Species

human

Clone Name

pFN21AE3186 Sequence (with vector) Restriction Map

Product ID

FHC27769

ORF Sequence

DNA sequence (1278 bp)
Protein sequence (426 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3186

Symbol

KCNK2

Alias : K2p2.1, TPKC1, TREK, TREK-1, TREK1, hTREK-1c, hTREK-1e

Description

potassium channel, two pore domain subfamily K, member 2, transcript variant 3

Original Clone ID

cp93186

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1278 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 426 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS41467.1 6.3e-174 100.0 KCNK2 CCDS41466.1 5.5e-167 97.4 KCNK2 CCDS31024.1 6.2e-167 99.8 KCNK2 CCDS9880.1 2e-97 64.8 KCNK10 CCDS9882.1 2e-97 64.8 KCNK10 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR003976 16 29 PR01499 Potassium channel IPR003976 53 70 PR01499 Potassium channel IPR003976 90 102 PR01499 Potassium channel IPR003976 104 122 PR01499 Potassium channel IPR003976 131 143 PR01499 Potassium channel IPR003280 153 181 PR01333 Potassium channel IPR003976 181 200 PR01499 Potassium channel IPR003976 201 220 PR01499 Potassium channel IPR003976 222 233 PR01499 Potassium channel IPR003280 265 274 PR01333 Potassium channel IPR003976 277 292 PR01499 Potassium channel IPR003976 299 310 PR01499 Potassium channel IPR003976 312 328 PR01499 Potassium channel IPR003976 348 362 PR01499 Potassium channel HMMPfam IPR013099 125 196 PF07885 Ion transport 2 IPR013099 234 312 PF07885 Ion transport 2
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 61 TVSTIFLVVVLYLIIGATVFKAL 83 PRIMARY 23 2 117 LIQQIVAAINAGIIPLGNTSNQI 139 SECONDARY 23 3 143 DLGSSFFFAGTVITTIGFGNISP 165 SECONDARY 23 4 171 KIFCIIYALLGIPLFGFLLAGVG 193 PRIMARY 23 5 222 RIISTIIFILFGCVLFVALPAII 244 PRIMARY 23 6 252 SALDAIYFVVITLTTIGFGDYVA 274 SECONDARY 23 7 289 VWFWILVGLAYFAAVLSMIGDWL 311 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001017425 426 603219 100.0 100.0 XP_016856737 422 603219 97.4 100.0 NP_001017424 422 603219 97.4 100.0 NP_055032 411 603219 99.8 96.5 XP_016856738 412 603219 99.8 96.5 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3186 Download

>KIEE3186  1278 bp
ATGCTTCCCAGCGCCTCGCGGGAGAGACCCGGCTATAGAGCAGGAGTGGCGGCACCTGAC
TTGCTGGATCCTAAATCTGCCGCTCAGAACTCCAAACCGAGGCTCTCGTTTTCCACGAAA
CCCACAGTGCTTGCTTCCCGGGTGGAGAGTGACACGACCATTAATGTTATGAAATGGAAG
ACGGTCTCCACGATATTCCTGGTGGTTGTCCTCTATCTGATCATCGGAGCCACCGTGTTC
AAAGCATTGGAGCAGCCTCATGAGATTTCACAGAGGACCACCATTGTGATCCAGAAGCAA
ACATTCATATCCCAACATTCCTGTGTCAATTCGACGGAGCTGGATGAACTCATTCAGCAA
ATAGTGGCAGCAATAAATGCAGGGATTATACCGTTAGGAAACACCTCCAATCAAATCAGT
CACTGGGATTTGGGAAGTTCCTTCTTCTTTGCTGGCACTGTTATTACAACCATAGGATTT
GGAAACATCTCACCACGCACAGAAGGCGGCAAAATATTCTGTATCATCTATGCCTTACTG
GGAATTCCCCTCTTTGGTTTTCTCTTGGCTGGAGTTGGAGATCAGCTAGGCACCATATTT
GGAAAAGGAATTGCCAAAGTGGAAGATACGTTTATTAAGTGGAATGTTAGTCAGACCAAG
ATTCGCATCATCTCAACAATCATATTTATACTATTTGGCTGTGTACTCTTTGTGGCTCTG
CCTGCGATCATATTCAAACACATAGAAGGCTGGAGTGCCCTGGACGCCATTTATTTTGTG
GTTATCACTCTAACAACTATTGGATTTGGTGACTACGTTGCAGGTGGATCCGATATTGAA
TATCTGGACTTCTATAAGCCTGTCGTGTGGTTCTGGATCCTTGTAGGGCTTGCTTACTTT
GCTGCTGTCCTGAGCATGATTGGAGATTGGCTCCGAGTGATATCTAAAAAGACAAAAGAA
GAGGTGGGAGAGTTCAGAGCACACGCTGCTGAGTGGACAGCCAACGTCACAGCCGAATTC
AAAGAAACCAGGAGGCGACTGAGTGTGGAGATTTATGACAAGTTCCAGCGGGCCACCTCC
ATCAAGCGGAAGCTCTCGGCAGAACTGGCTGGAAACCACAATCAGGAGCTGACTCCTTGT
AGGAGGACCCTGTCAGTGAACCACCTGACCAGCGAGAGGGATGTCTTGCCTCCCTTACTG
AAGACTGAGAGTATCTATCTGAATGGTTTGACGCCACACTGTGCTGGTGAAGAGATTGCT
GTGATTGAGAACATCAAA

ORF Protein

Cloned ORF protein sequence for pFN21AE3186 Download

>KIEE3186  426 aa
MLPSASRERPGYRAGVAAPDLLDPKSAAQNSKPRLSFSTKPTVLASRVESDTTINVMKWK
TVSTIFLVVVLYLIIGATVFKALEQPHEISQRTTIVIQKQTFISQHSCVNSTELDELIQQ
IVAAINAGIIPLGNTSNQISHWDLGSSFFFAGTVITTIGFGNISPRTEGGKIFCIIYALL
GIPLFGFLLAGVGDQLGTIFGKGIAKVEDTFIKWNVSQTKIRIISTIIFILFGCVLFVAL
PAIIFKHIEGWSALDAIYFVVITLTTIGFGDYVAGGSDIEYLDFYKPVVWFWILVGLAYF
AAVLSMIGDWLRVISKKTKEEVGEFRAHAAEWTANVTAEFKETRRRLSVEIYDKFQRATS
IKRKLSAELAGNHNQELTPCRRTLSVNHLTSERDVLPPLLKTESIYLNGLTPHCAGEEIA
VIENIK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3186 Download

>pFN21AE3186  5983 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCTTCCCAGCGCCTCGCGGGAGAGACCCGGCTA
TAGAGCAGGAGTGGCGGCACCTGACTTGCTGGATCCTAAATCTGCCGCTCAGAACTCCAA
ACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTTGCTTCCCGGGTGGAGAGTGACAC
GACCATTAATGTTATGAAATGGAAGACGGTCTCCACGATATTCCTGGTGGTTGTCCTCTA
TCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAGCAGCCTCATGAGATTTCACAGAG
GACCACCATTGTGATCCAGAAGCAAACATTCATATCCCAACATTCCTGTGTCAATTCGAC
GGAGCTGGATGAACTCATTCAGCAAATAGTGGCAGCAATAAATGCAGGGATTATACCGTT
AGGAAACACCTCCAATCAAATCAGTCACTGGGATTTGGGAAGTTCCTTCTTCTTTGCTGG
CACTGTTATTACAACCATAGGATTTGGAAACATCTCACCACGCACAGAAGGCGGCAAAAT
ATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTCTTTGGTTTTCTCTTGGCTGGAGT
TGGAGATCAGCTAGGCACCATATTTGGAAAAGGAATTGCCAAAGTGGAAGATACGTTTAT
TAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATCTCAACAATCATATTTATACTATT
TGGCTGTGTACTCTTTGTGGCTCTGCCTGCGATCATATTCAAACACATAGAAGGCTGGAG
TGCCCTGGACGCCATTTATTTTGTGGTTATCACTCTAACAACTATTGGATTTGGTGACTA
CGTTGCAGGTGGATCCGATATTGAATATCTGGACTTCTATAAGCCTGTCGTGTGGTTCTG
GATCCTTGTAGGGCTTGCTTACTTTGCTGCTGTCCTGAGCATGATTGGAGATTGGCTCCG
AGTGATATCTAAAAAGACAAAAGAAGAGGTGGGAGAGTTCAGAGCACACGCTGCTGAGTG
GACAGCCAACGTCACAGCCGAATTCAAAGAAACCAGGAGGCGACTGAGTGTGGAGATTTA
TGACAAGTTCCAGCGGGCCACCTCCATCAAGCGGAAGCTCTCGGCAGAACTGGCTGGAAA
CCACAATCAGGAGCTGACTCCTTGTAGGAGGACCCTGTCAGTGAACCACCTGACCAGCGA
GAGGGATGTCTTGCCTCCCTTACTGAAGACTGAGAGTATCTATCTGAATGGTTTGACGCC
ACACTGTGCTGGTGAAGAGATTGCTGTGATTGAGAACATCAAAGTTTAAACGAATTCGGG
CTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTA
ACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAAC
CCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
ACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTA
TTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATG
TTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGT
GGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCC
CAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATG
GTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTT
CAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCC
TTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAA
GATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGT
AAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTT
CTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGC
ATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACG
GATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCG
GCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAAC
ATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCA
AACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTA
ACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGAT
AAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAA
TCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAG
CCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAAT
AGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGAC
CAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTA
AAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTC
CCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGT
CCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA
GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCG
ACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTAT
CGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTA
CAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAAC
AAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA
AAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAA
ACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTT
TATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCAT
GAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCA
TAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTG
TTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACA
ATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGC
AGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT