(Linear) (Six-base) MAPSORT of: KIEE3186.seq  Check: 8110  from: 1  to: 1278



KIEE3186.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     811    1278 
   Size:        811     467
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1219    1278 
   Size:       1219      59
 
AleI CACnn'nnGTG

Cuts at:      0     283    1278 
   Size:        283     995
 
AlwNI CAG_nnn'CTG

Cuts at:      0     422    1128    1278 
   Size:        422     706     150

  Fragments arranged by size: 

                 706     422     150
 
ApoI r'AATT_y

Cuts at:      0     542    1015    1278 
   Size:        542     473     263

  Fragments arranged by size: 

                 542     473     263
 
AseI AT'TA_AT

Cuts at:      0     161    1278 
   Size:        161    1117
 
AvaI C'yCGr_G

Cuts at:      0     137    1278 
   Size:        137    1141
 
BamHI G'GATC_C

Cuts at:      0      66     826     875    1278 
   Size:         66     760      49     403

  Fragments arranged by size: 

                 760     403      66      49
 
BanI G'GyrC_C

Cuts at:      0      51     589    1278 
   Size:         51     538     689

  Fragments arranged by size: 

                 689     538      51
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     184    1207    1278 
   Size:        184    1023      71

  Fragments arranged by size: 

                1023     184      71
 
BclI T'GATC_A

Cuts at:      0     218    1278 
   Size:        218    1060
 
Bme1580I G_kGCm'C

Cuts at:      0     759    1278 
   Size:        759     519
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     432     547    1278 
   Size:        432     115     731

  Fragments arranged by size: 

                 731     432     115
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     946    1278 
   Size:        946     332
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     771    1278 
   Size:        590     181     507

  Fragments arranged by size: 

                 590     507     181
 
Bpu10I CC'TnA_GC

Cuts at:      0     910    1278 
   Size:        910     368
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      18     189    1278 
   Size:         18     171    1089

  Fragments arranged by size: 

                1089     171      18
 
BsaBI GATnn'nnATC

Cuts at:      0     664    1278 
   Size:        664     614
 
BsaHI Gr'CG_yC

Cuts at:      0     764    1231    1278 
   Size:        764     467      47

  Fragments arranged by size: 

                 764     467      47
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     218     248    1278 
   Size:        218      30    1030

  Fragments arranged by size: 

                1030     218      30
 
BseYI C'CCAG_C

Cuts at:      0       7    1278 
   Size:          7    1271
 
BsiHKAI G_wGCw'C

Cuts at:      0     981    1278 
   Size:        981     297
 
Bsp1286I G_dGCh'C

Cuts at:      0     759     981    1278 
   Size:        759     222     297

  Fragments arranged by size: 

                 759     297     222
 
BspHI T'CATG_A

Cuts at:      0     258    1278 
   Size:        258    1020
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     811    1278 
   Size:        811     467
 
BsrBI CCG'CTC

Cuts at:      0      82    1278 
   Size:         82    1196
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     376     985    1278 
   Size:        376     609     293

  Fragments arranged by size: 

                 609     376     293
 
BstYI r'GATC_y

Cuts at:      0      66     826     875    1278 
   Size:         66     760      49     403

  Fragments arranged by size: 

                 760     403      66      49
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     111     146    1278 
   Size:        111      35    1132

  Fragments arranged by size: 

                1132     111      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1240    1278 
   Size:       1240      38
 
EarI CTCTTCn'nnn_

Cuts at:      0     953    1244    1278 
   Size:        953     291      34

  Fragments arranged by size: 

                 953     291      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     590     771    1219    1278 
   Size:        590     181     448      59

  Fragments arranged by size: 

                 590     448     181      59
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1145    1278 
   Size:       1145     133
 
EcoRI G'AATT_C

Cuts at:      0     542    1015    1278 
   Size:        542     473     263

  Fragments arranged by size: 

                 542     473     263
 
EcoRV GAT'ATC

Cuts at:      0     941    1278 
   Size:        941     337
 
HaeII r_GCGC'y

Cuts at:      0      14    1278 
   Size:         14    1264
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90    1200    1232    1278 
   Size:         58      32    1110      32      46

  Fragments arranged by size: 

                1110      58      46      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     555    1278 
   Size:        555     723
 
MslI CAynn'nnrTG

Cuts at:      0     283     320    1278 
   Size:        283      37     958

  Fragments arranged by size: 

                 958     283      37
 
MspA1I CmG'CkG

Cuts at:      0    1068    1278 
   Size:       1068     210
 
PpuMI rG'GwC_Cy

Cuts at:      0    1145    1278 
   Size:       1145     133
 
SfcI C'TryA_G

Cuts at:      0      33    1278 
   Size:         33    1245
 
SmaI CCC'GGG

Cuts at:      0     139    1278 
   Size:        139    1139
 
SspI AAT'ATT

Cuts at:      0     515    1278 
   Size:        515     763
 
TatI w'GTAC_w

Cuts at:      0     702    1278 
   Size:        702     576
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     218     250    1278 
   Size:        218      32    1028

  Fragments arranged by size: 

                1028     218      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1149    1278 
   Size:       1149     129
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     195     774    1278 
   Size:        195     579     504

  Fragments arranged by size: 

                 579     504     195
 
XmaI C'CCGG_G

Cuts at:      0     137    1278 
   Size:        137    1141
 
XmnI GAAnn'nnTTC

Cuts at:      0     350    1278 
   Size:        350     928
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApoI     AseI     AvaI    BamHI
     BanI     BbsI     BclI Bme1580I     BmrI     BplI     BpmI   Bpu10I
     BsaI    BsaBI    BsaHI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspHI
    BspMI    BsrBI   BstAPI    BstYI    CspCI   DraIII     EarI  Eco57MI
 EcoO109I    EcoRI    EcoRV    HaeII    Hin4I     MmeI     MslI   MspA1I
    PpuMI     SfcI     SmaI     SspI     TatI     TstI  Tth111I     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI    BpuEI    BsaAI    BsaWI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     XbaI
     XhoI     ZraI