Restriction map for
pFN21AE3186
(Circular) (Six-base) MAPSORT of: pFN21AE3186.seq Check: 6939 from: 1 to: 5983
pFN21AE3186 5983 bp
With 182 enzymes: SgfI *
March 28, 2012 12:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2816 2816
Size: 5983
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 278
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3326 1937
Size: 1389 4594
Acc65I G'GTAC_C
Cuts at: 3304 3304
Size: 5983
AclI AA'CG_TT
Cuts at: 1563 4110 4483 1563
Size: 2547 373 3063
Fragments arranged by size:
3063 2547 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3224 4046 5430 5698 1632
Size: 1592 822 1384 268 1917
Fragments arranged by size:
1917 1592 1384 822 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5786
AflIII A'CryG_T
Cuts at: 1203 4882 5859 1203
Size: 3679 977 1327
Fragments arranged by size:
3679 1327 977
AgeI A'CCGG_T
Cuts at: 4814 4814
Size: 5983
AhdI GACnn_n'nnGTC
Cuts at: 4712 4712
Size: 5983
AleI CACnn'nnGTG
Cuts at: 1166 2288 1166
Size: 1122 4861
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5983
AlwNI CAG_nnn'CTG
Cuts at: 2427 3133 3726 5298 2427
Size: 706 593 1572 3112
Fragments arranged by size:
3112 1572 706 593
ApaLI G'TGCA_C
Cuts at: 4040 5196 4040
Size: 1156 4827
ApoI r'AATT_y
Cuts at: 1493 2547 3020 3292 3518 3670 5779 1493
Size: 1054 473 272 226 152 2109 1697
Fragments arranged by size:
2109 1697 1054 473 272 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2166 4537 160
Size: 872 1134 2371 1606
Fragments arranged by size:
2371 1606 1134 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
AvaI C'yCGr_G
Cuts at: 1870 1943 2142 3308 1870
Size: 73 199 1166 4545
Fragments arranged by size:
4545 1166 199 73
BamHI G'GATC_C
Cuts at: 2071 2831 2880 3313 3680 2071
Size: 760 49 433 367 4374
Fragments arranged by size:
4374 760 433 367 49
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2056 2594 3304 4759 618
Size: 325 217 627 269 538 710 1455 1842
Fragments arranged by size:
1842 1455 710 627 538 325 269 217
BanII G_rGCy'C
Cuts at: 1391 3302 1391
Size: 1911 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2189 3212 928
Size: 971 290 1023 3699
Fragments arranged by size:
3699 1023 971 290
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4174 4208 1903
Size: 34 50 34 2153 34 3678
Fragments arranged by size:
3678 2153 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3875 5085 3875
Size: 1210 4773
BclI T'GATC_A
Cuts at: 1548 2223 1548
Size: 675 5308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4594 136
Size: 107 122 71 631 605 2922 1525
Fragments arranged by size:
2922 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5978 5978
Size: 5983
BlpI GC'TnA_GC
Cuts at: 3400 3400
Size: 5983
Bme1580I G_kGCm'C
Cuts at: 1790 2764 4044 5200 1790
Size: 974 1280 1156 2573
Fragments arranged by size:
2573 1280 1156 974
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5983
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2437 2552 3714
Size: 416 53 83 399 24 1020 115 1162
Cuts at: 3714 4672 442
Size: 958 1753
Fragments arranged by size:
1753 1162 1020 958 416 399 115 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2951
Size: 5983
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2595 2776 4643 1239
Size: 1356 181 1867 2579
Fragments arranged by size:
2579 1867 1356 181
Bpu10I CC'TnA_GC
Cuts at: 2915 2915
Size: 5983
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4108 4973 5271 4108
Size: 865 298 4820
Fragments arranged by size:
4820 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2023 2194 4646 882
Size: 617 524 171 2452 2219
Fragments arranged by size:
2452 2219 617 524 171
BsaAI yAC'GTr
Cuts at: 493 1204 3774 493
Size: 711 2570 2702
Fragments arranged by size:
2702 2570 711
BsaBI GATnn'nnATC
Cuts at: 2669 5977 2669
Size: 3308 2675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2769 3236 3790 4172
Size: 53 83 186 936 1236 467 554 382
Cuts at: 4172 4807 275
Size: 635 1451
Fragments arranged by size:
1451 1236 936 635 554 467 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4416 4814 5088 5235 5646 4416
Size: 398 274 147 411 4753
Fragments arranged by size:
4753 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2223 2253 1178
Size: 30 165 30 820 30 4908
Fragments arranged by size:
4908 820 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5983
BseYI C'CCAG_C
Cuts at: 2012 5186 5682 2012
Size: 3174 496 2313
Fragments arranged by size:
3174 2313 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5410
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3433 4194 5222 1540
Size: 275 186 1432 761 1028 2301
Fragments arranged by size:
2301 1432 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2986 4044 4129 5200 2986
Size: 1058 85 1071 3769
Fragments arranged by size:
3769 1071 1058 85
BsmI GAATG_Cn'
Cuts at: 3494 3587 3494
Size: 93 5890
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2764 2986 3302 4044 4129 5200 1391
Size: 399 974 222 316 742 85 1071 2174
Fragments arranged by size:
2174 1071 974 742 399 316 222 85
BspEI T'CCGG_A
Cuts at: 5646 5646
Size: 5983
BspHI T'CATG_A
Cuts at: 2263 3872 5602 2263
Size: 1609 1730 2644
Fragments arranged by size:
2644 1730 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 3338 844
Size: 1972 522 3489
Fragments arranged by size:
3489 1972 522
BsrBI CCG'CTC
Cuts at: 1394 1823 2087 3870 1394
Size: 429 264 1783 3507
Fragments arranged by size:
3507 1783 429 264
BsrDI GCAATG_nn'
Cuts at: 65 1248 4478 4652 65
Size: 1183 3230 174 1396
Fragments arranged by size:
3230 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4627 4814 1700
Size: 110 2817 187 2869
Fragments arranged by size:
2869 2817 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5983
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5983
BssSI C'ACGA_G
Cuts at: 4043 5055 4043
Size: 1012 4971
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2381 2990 794
Size: 1587 609 3787
Fragments arranged by size:
3787 1587 609
BstBI TT'CG_AA
Cuts at: 4787 4787
Size: 5983
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5983
BstYI r'GATC_y
Cuts at: 1972 2071 2831 2880 3313 3680 4065 4082 5534
Size: 99 760 49 433 367 385 17 1452
Cuts at: 5534 5620 5632 5978 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 760 433 385 367 346 99
86 49 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5431
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5244
Fragments arranged by size:
5244 683 56
BtsI GCAGTG_nn'
Cuts at: 3499 4293 4313 3499
Size: 794 20 5169
Fragments arranged by size:
5169 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5983
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2116 2151 529
Size: 35 1552 35 4361
Fragments arranged by size:
4361 1552 35 35
DraI TTT'AAA
Cuts at: 3287 3287
Size: 5983
DraIII CAC_nnn'GTG
Cuts at: 3245 3245
Size: 5983
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4990 809
Size: 507 3674 1802
Fragments arranged by size:
3674 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3430 4319 5948
Size: 54 1425 50 275 18 1600 889 1629
Cuts at: 5948 8
Size: 43
Fragments arranged by size:
1629 1600 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3430 1537
Size: 275 1618 4090
Fragments arranged by size:
4090 1618 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2958 3249 3913 1353
Size: 1605 291 664 3423
Fragments arranged by size:
3423 1605 664 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4570 4944 5090 387
Size: 1064 138 2981 374 146 1280
Fragments arranged by size:
2981 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2595 2776 3224 4046 4643 5430 5698
Size: 393 963 181 448 822 597 787 268
Cuts at: 5698 1239
Size: 1524
Fragments arranged by size:
1524 963 822 787 597 448 393 268
181
EcoO109I rG'GnC_Cy
Cuts at: 3150 3150
Size: 5983
EcoRI G'AATT_C
Cuts at: 2547 3020 3292 3670 2547
Size: 473 272 378 4860
Fragments arranged by size:
4860 473 378 272
EcoRV GAT'ATC
Cuts at: 2946 2946
Size: 5983
FspI TGC'GCA
Cuts at: 4489 4489
Size: 5983
HaeII r_GCGC'y
Cuts at: 1678 2019 5130 1678
Size: 341 3111 2531
Fragments arranged by size:
3111 2531 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2063 2095 3205 3237 4624
Size: 32 135 32 491 32 1110 32 1387
Cuts at: 4624 4656 4698 4730 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1387 1110 491 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 3573 669
Size: 988 281 1389 246 3079
Fragments arranged by size:
3079 1389 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5983
HpaI GTT'AAC
Cuts at: 3573 3573
Size: 5983
KpnI G_GTAC'C
Cuts at: 3308 3308
Size: 5983
MfeI C'AATT_G
Cuts at: 3582 3582
Size: 5983
MluI A'CGCG_T
Cuts at: 5859 5859
Size: 5983
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2560 5097 5281 1305
Size: 1255 2537 184 2007
Fragments arranged by size:
2537 2007 1255 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4161
Fragments arranged by size:
4161 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2288 2325 3941 4300 4459 518
Size: 648 1122 37 1616 359 159 2042
Fragments arranged by size:
2042 1616 1122 648 359 159 37
MspA1I CmG'CkG
Cuts at: 1606 3073 3399 4076 5224 5469 1606
Size: 1467 326 677 1148 245 2120
Fragments arranged by size:
2120 1467 1148 677 326 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5431
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5983
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5983
NotI GC'GGCC_GC
Cuts at: 3430 3430
Size: 5983
NspI r_CATG'y
Cuts at: 1132 1229 3341 4886 1132
Size: 97 2112 1545 2229
Fragments arranged by size:
2229 2112 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5983
PciI A'CATG_T
Cuts at: 4882 4882
Size: 5983
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5983
PmeI GTTT'AAAC
Cuts at: 3287 3287
Size: 5983
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4052 4084 5565 5597 4052
Size: 32 1481 32 4438
Fragments arranged by size:
4438 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3150 3150
Size: 5983
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5983
PsiI TTA'TAA
Cuts at: 3553 3553
Size: 5983
PstI C_TGCA'G
Cuts at: 830 3335 830
Size: 2505 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5983
SalI G'TCGA_C
Cuts at: 1936 3325 1936
Size: 1389 4594
SbfI CC_TGCA'GG
Cuts at: 3335 3335
Size: 5983
ScaI AGT'ACT
Cuts at: 4231 5943 4231
Size: 1712 4271
SfcI C'TryA_G
Cuts at: 826 1045 2038 3331 4466 5147 5338 826
Size: 219 993 1293 1135 681 191 1471
Fragments arranged by size:
1471 1293 1135 993 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5983
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5983
SmaI CCC'GGG
Cuts at: 1872 2144 3310 1872
Size: 272 1166 4545
Fragments arranged by size:
4545 1166 272
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4087 4988 5250 820
Size: 197 926 2144 901 262 1553
Fragments arranged by size:
2144 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3774 493
Size: 3281 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5983
SphI G_CATG'C
Cuts at: 1132 3341 1132
Size: 2209 3774
SspI AAT'ATT
Cuts at: 5 52 2520 3907 5
Size: 47 2468 1387 2081
Fragments arranged by size:
2468 2081 1387 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5983
StyI C'CwwG_G
Cuts at: 513 1065 3422 513
Size: 552 2357 3074
Fragments arranged by size:
3074 2357 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4010 4027 4180 4365 4810 325
Size: 1255 2430 17 153 185 445 1498
Fragments arranged by size:
2430 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2707 4229 5941
Size: 274 80 33 51 1443 730 1522 1712
Cuts at: 5941 96
Size: 138
Fragments arranged by size:
1712 1522 1443 730 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2223 2255 5943 5975 2223
Size: 32 3688 32 2231
Fragments arranged by size:
3688 2231 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3154 1316
Size: 1838 4145
XbaI T'CTAG_A
Cuts at: 3319 3319
Size: 5983
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2200 2779 2200
Size: 579 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5983
XmaI C'CCGG_G
Cuts at: 1870 2142 3308 1870
Size: 272 1166 4545
Fragments arranged by size:
4545 1166 272
XmnI GAAnn'nnTTC
Cuts at: 2355 4112 2355
Size: 1757 4226
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 276
Size: 53 83 186 936 2257 2468
Fragments arranged by size:
2468 2257 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI