################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 17 CGCG CG^CG AfaI 703 GTAC GT^AC AseI 160 ATTAAT AT^TAAT AsnI 160 ATTAAT AT^TAAT Asp700I 346 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 977 GAGCAC G[AT]GC[AT]^C AspI 1146 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 137 CCCGGG C^[CT]CG[GA]G BclI 218 TGATCA T^GATCA BfaI 586 CTAG C^TAG BsaBI 660 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 977 GAGCAC G[AT]GC[AT]^C BsoBI 137 CCCGGG C^[CT]CG[GA]G BspHI 258 TCATGA T^CATGA BspMI 820 GCAGGT ACCTGC(4/8) BsrBI 80 CCGCTC CCGCTC(-3/-3) BstUI 17 CGCG CG^CG CfoI 11 GCGC GCG^C DraII 1144 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 1235 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 1144 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRV 939 GATATC GAT^ATC FbaI 218 TGATCA T^GATCA HaeII 10 AGCGCC [GA]GCGC^[CT] HaeIII 1071 GGCC GG^CC HhaI 11 GCGC GCG^C HinP1I 11 GCGC G^CGC MaeI 586 CTAG C^TAG MamI 660 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 1066 CAGCGG C[AC]G^C[GT]G MvnI 17 CGCG CG^CG PflFI 1146 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1131 GACTC GAGTC(4/5) PpuMI 1144 AGGACCC [GA]G^G[AT]CC[CT] PshBI 160 ATTAAT AT^TAAT RcaI 258 TCATGA T^CATGA RsaI 703 GTAC GT^AC SfaNI 665 GCATC GCATC(5/9) SfcI 33 CTATAG C^T[GA][CT]AG SmaI 137 CCCGGG CCC^GGG SspI 513 AATATT AAT^ATT TaqI 331 TCGA T^CGA TfiI 660 GATTC G^A[AT]TC Tth111I 1146 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 331 TCGA T^CGA XmaCI 137 CCCGGG C^CCGGG XmaI 137 CCCGGG C^CCGGG XmnI 346 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcsI 542 GAATTC [GA]^AATT[CT] 1015 GAATTC AcyI 763 GACGCC G[GA]^CG[CT]C 1230 GACGCC AlwNI 417 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 1123 CAGGAGCTG ApoI 542 GAATTC [GA]^AATT[CT] 1015 GAATTC AvaII 275 GGACC G^G[AT]CC 1145 GGACC BanI 51 GGCACC G^G[CT][GA]CC 589 GGCACC BbsI 177 GAAGAC GAAGAC(2/6) 1200 GAAGAC BmyI 755 GTGCCC G[AGT]GC[ACT]^C 977 GAGCAC BpmI 569 CTGGAG CTGGAG(16/14) 750 CTGGAG BpuAI 177 GAAGAC GAAGAC(2/6) 1200 GAAGAC BsaHI 763 GACGCC G[GA]^CG[CT]C 1230 GACGCC BsaI 24 GAGACC GGTCTC(1/5) 183 GGTCTC BsmAI 24 GAGAC GTCTC(1/5) 184 GTCTC Bsp1286I 755 GTGCCC G[AGT]GC[ACT]^C 977 GAGCAC BstAPI 370 GCAATAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 979 GCACACGCTGC BstF5I 342 GGATG GGATG(2/0) 1179 GGATG EarI 958 GAAGAG CTCTTC(1/4) 1249 GAAGAG EcoRI 542 GAATTC G^AATTC 1015 GAATTC FauI 18 GCGGG CCCGC(4/6) 1068 GCGGG FokI 342 GGATG GGATG(9/13) 1179 GGATG HapII 29 CCGG C^CGG 138 CCGG HgaI 763 GACGC GACGC(5/10) 1230 GACGC Hin1I 763 GACGCC G[GA]^CG[CT]C 1230 GACGCC HinfI 660 GATTC G^A[ACGT]TC 1131 GACTC HpaII 29 CCGG C^CGG 138 CCGG Ksp632I 958 GAAGAG CTCTTC(1/4) 1249 GAAGAG MseI 161 TTAA T^TAA 635 TTAA MslI 279 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 316 CATTCCTGTG MspI 29 CCGG C^CGG 138 CCGG NlaIII 259 CATG CATG^ 915 CATG Tru9I 161 TTAA T^TAA 635 TTAA XcmI 188 CCACGATATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 767 CCATTTATTTTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AluI AlwI BamHI BbvI BcnI BsaJI BsiYI BslI BsrI BssKI BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI Tsp45I Tsp509I TspRI XhoII