RNF133 ORF clone pFN21AE3158

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Halotag ORF Clone pFN21AE3158

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Clone info.

Species

human

Clone Name

pFN21AE3158 Sequence (with vector) Restriction Map

Product ID

FHC23068

ORF Sequence

DNA sequence (1128 bp)
Protein sequence (376 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE3158

Symbol

RNF133

Description

ring finger protein 133

Original Clone ID

cp93158

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1128 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 376 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS5784.1 8.5e-144 100.0 RNF133 CCDS47692.1 6.6e-64 55.4 RNF148 CCDS14520.1 1.3e-56 44.7 RNF128 CCDS14521.1 4.3e-52 50.1 RNF128 CCDS34065.1 1.8e-35 32.5 RNF150 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR003137 102 163 PF02225 Protease-associated domain IPR018957 256 296 PF00097 Zinc finger HMMSmart IPR001841 256 296 SM00184 Zinc finger ProfileScan IPR001841 256 297 PS50089 Zinc finger
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 20 KFSVLWLVSQNCCRASVVWMAYM 42 SECONDARY 23 2 159 KGTEIFHLIKKGVLITAVVEVG 180 SECONDARY 22 3 185 IWMNHYLVSFVIVTTATLAYFIF 207 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_078815 402 300439 44.7 96.8 NP_919445 428 300439 50.1 90.7 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE3158 Download

>KIEE3158  1128 bp
ATGCATCTACTCAAGGTTGGCACTTGGAGAAACAACACTGCCTCTTCCTGGCTTATGAAG
TTCAGTGTTCTTTGGCTTGTTAGTCAGAACTGTTGCAGAGCAAGTGTTGTTTGGATGGCT
TATATGAACATATCATTTCATGTTGGGAATCATGTGTTGTCAGAGTTGGGAGAGACTGGA
GTCTTTGGAAGAAGCTCCACTTTGAAGAGAGTGGCAGGAGTTATAGTGCCACCAGAGGGA
AAAATCCAAAATGCATGTAATCCCAATACCATTTTCAGCCGATCAAAGTACTCAGAGACC
TGGCTTGCACTTATTGAACGGGGAGGTTGTACCTTCACACAGAAAATTAAAGTGGCAACT
GAGAAGGGAGCCAGTGGAGTGATCATCTATAACGTTCCAGGTACTGGCAACCAGGTGTTC
CCCATGTTTCATCAGGCATTTGAAGATGTCGTTGTGGTTATGATTGGTAACTTAAAAGGC
ACGGAAATTTTCCATTTAATTAAGAAGGGAGTTCTCATTACAGCCGTGGTTGAGGTGGGG
AGAAAGCACATCATCTGGATGAATCACTATTTGGTCTCTTTTGTGATTGTCACAACTGCT
ACCTTAGCATATTTCATCTTTTATCACATTCATAGACTTTGTTTAGCAAGGATTCAGAAC
CGGAGATGGCAGCGATTAACAACAGATCTTCAGAACACATTTGGACAACTCCAACTTCGA
GTAGTAAAAGAGGGGGATGAAGAAATAAATCCAAATGGGGATAGCTGCGTAATTTGCTTT
GAACGCTATAAGCCTAATGACATAGTTCGTATTCTGACTTGTAAACATTTTTTCCACAAG
AATTGCATTGACCCCTGGATTTTACCCCATGGGACATGCCCCATTTGCAAATGTGATATT
CTTAAAGTTTTGGGGATTCAAGTGGTTGTTGAAAATGGAACAGAACCTTTGCAAGTTCTA
ATGTCAAATGAACTGCCTGAAACCTTATCACCTAGTGAAGAGGAGACAAATAATGAAGTT
TCTCCTGCAGGAACCTCAGATAAAGTAATCCATGTGGAGGAGAACCCTACTTCTCAGAAT
AATGACATCCAGCCTCATTCAGTAGTGGAAGATGTTCATCCTTCACCT

ORF Protein

Cloned ORF protein sequence for pFN21AE3158 Download

>KIEE3158  376 aa
MHLLKVGTWRNNTASSWLMKFSVLWLVSQNCCRASVVWMAYMNISFHVGNHVLSELGETG
VFGRSSTLKRVAGVIVPPEGKIQNACNPNTIFSRSKYSETWLALIERGGCTFTQKIKVAT
EKGASGVIIYNVPGTGNQVFPMFHQAFEDVVVVMIGNLKGTEIFHLIKKGVLITAVVEVG
RKHIIWMNHYLVSFVIVTTATLAYFIFYHIHRLCLARIQNRRWQRLTTDLQNTFGQLQLR
VVKEGDEEINPNGDSCVICFERYKPNDIVRILTCKHFFHKNCIDPWILPHGTCPICKCDI
LKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVIHVEENPTSQN
NDIQPHSVVEDVHPSP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE3158 Download

>pFN21AE3158  5833 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCATCTACTCAAGGTTGGCACTTGGAGAAACAA
CACTGCCTCTTCCTGGCTTATGAAGTTCAGTGTTCTTTGGCTTGTTAGTCAGAACTGTTG
CAGAGCAAGTGTTGTTTGGATGGCTTATATGAACATATCATTTCATGTTGGGAATCATGT
GTTGTCAGAGTTGGGAGAGACTGGAGTCTTTGGAAGAAGCTCCACTTTGAAGAGAGTGGC
AGGAGTTATAGTGCCACCAGAGGGAAAAATCCAAAATGCATGTAATCCCAATACCATTTT
CAGCCGATCAAAGTACTCAGAGACCTGGCTTGCACTTATTGAACGGGGAGGTTGTACCTT
CACACAGAAAATTAAAGTGGCAACTGAGAAGGGAGCCAGTGGAGTGATCATCTATAACGT
TCCAGGTACTGGCAACCAGGTGTTCCCCATGTTTCATCAGGCATTTGAAGATGTCGTTGT
GGTTATGATTGGTAACTTAAAAGGCACGGAAATTTTCCATTTAATTAAGAAGGGAGTTCT
CATTACAGCCGTGGTTGAGGTGGGGAGAAAGCACATCATCTGGATGAATCACTATTTGGT
CTCTTTTGTGATTGTCACAACTGCTACCTTAGCATATTTCATCTTTTATCACATTCATAG
ACTTTGTTTAGCAAGGATTCAGAACCGGAGATGGCAGCGATTAACAACAGATCTTCAGAA
CACATTTGGACAACTCCAACTTCGAGTAGTAAAAGAGGGGGATGAAGAAATAAATCCAAA
TGGGGATAGCTGCGTAATTTGCTTTGAACGCTATAAGCCTAATGACATAGTTCGTATTCT
GACTTGTAAACATTTTTTCCACAAGAATTGCATTGACCCCTGGATTTTACCCCATGGGAC
ATGCCCCATTTGCAAATGTGATATTCTTAAAGTTTTGGGGATTCAAGTGGTTGTTGAAAA
TGGAACAGAACCTTTGCAAGTTCTAATGTCAAATGAACTGCCTGAAACCTTATCACCTAG
TGAAGAGGAGACAAATAATGAAGTTTCTCCTGCAGGAACCTCAGATAAAGTAATCCATGT
GGAGGAGAACCCTACTTCTCAGAATAATGACATCCAGCCTCATTCAGTAGTGGAAGATGT
TCATCCTTCACCTGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGAC
CTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTG
CTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATG
ATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTT
ATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAA
GTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTT
TTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTT
GCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCAC
CGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGG
GGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG
CTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGT
ATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTT
GCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTG
GGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAA
CGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATT
GACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAG
TACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGT
GCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGA
CCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGT
TGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTA
GCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGG
CAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCC
CTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGT
ATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACG
GGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTG
ATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACT
CAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAG
CAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA
GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACC
CGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG
TTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGC
TTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTC
TTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGA
TTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG
GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAA
AAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTG
TTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTT
CTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTG
GATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGG
GGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGG
CGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGAT
CGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTG
TCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAG
AGGATCGAGATCT