(Circular) (Six-base) MAPSORT of: pFN21AE3158.seq Check: 1282 from: 1 to: 5833 pFN21AE3158 5833 bp With 182 enzymes: SgfI * March 28, 2012 12:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 2397 3960 4333 1563 Size: 834 1563 373 3063 Fragments arranged by size: 3063 1563 834 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 3896 5280 5548 1632 Size: 1046 1218 1384 268 1917 Fragments arranged by size: 1917 1384 1218 1046 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 4732 5709 1203 Size: 3529 977 1327 Fragments arranged by size: 3529 1327 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AlwNI CAG_nnn'CTG Cuts at: 2408 3576 5148 2408 Size: 1168 1572 3093 Fragments arranged by size: 3093 1572 1168 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2490 3142 3368 3520 5629 1493 Size: 997 652 226 152 2109 1697 Fragments arranged by size: 2109 1697 997 652 226 152 AseI AT'TA_AT Cuts at: 160 1032 4387 160 Size: 872 3355 1606 Fragments arranged by size: 3355 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 3158 1870 Size: 73 1215 4545 Fragments arranged by size: 4545 1215 73 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4609 618 Size: 325 217 627 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 627 325 217 BanII G_rGCy'C Cuts at: 1391 3152 1391 Size: 1761 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4024 4058 1903 Size: 34 50 34 2003 34 3678 Fragments arranged by size: 3678 2003 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 2385 1548 Size: 837 4996 BfrBI ATG'CAT Cuts at: 2008 2258 2008 Size: 250 5583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4444 136 Size: 107 122 71 631 605 2772 1525 Fragments arranged by size: 2772 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2689 5828 2689 Size: 3139 2694 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 3894 5050 1790 Size: 2104 1156 2573 Fragments arranged by size: 2573 2104 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2202 4493 1239 Size: 963 2291 2579 Fragments arranged by size: 2579 2291 963 Bpu10I CC'TnA_GC Cuts at: 2608 2608 Size: 5833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2000 3958 4823 5121 2000 Size: 1958 865 298 2712 Fragments arranged by size: 2712 1958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2294 2584 4496 882 Size: 617 795 290 1912 2219 Fragments arranged by size: 2219 1912 795 617 290 BsaAI yAC'GTr Cuts at: 493 1204 3624 493 Size: 711 2420 2702 Fragments arranged by size: 2702 2420 711 BsaBI GATnn'nnATC Cuts at: 3120 5827 3120 Size: 2707 3126 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2664 4266 4664 4938 5085 5496 2664 Size: 1602 398 274 147 411 3001 Fragments arranged by size: 3001 1602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2214 2244 1178 Size: 30 165 30 811 30 4767 Fragments arranged by size: 4767 811 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3020 3077 1186 Size: 1834 57 3942 Fragments arranged by size: 3942 1834 57 BseYI C'CCAG_C Cuts at: 5036 5532 5036 Size: 496 5337 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5260 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3894 3979 5050 3894 Size: 85 1071 4677 Fragments arranged by size: 4677 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3152 3894 3979 5050 1391 Size: 399 1362 742 85 1071 2174 Fragments arranged by size: 2174 1362 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 3722 5452 3722 Size: 1730 4103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 844 Size: 2344 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3720 1394 Size: 429 1897 3507 Fragments arranged by size: 3507 1897 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4328 4502 65 Size: 1183 3080 174 1396 Fragments arranged by size: 3080 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5833 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2689 3163 3530 3915 3932 5384 5470 5482 Size: 717 474 367 385 17 1452 86 12 Cuts at: 5482 5828 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 717 474 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2529 2872 513 Size: 552 1464 343 3474 Fragments arranged by size: 3474 1464 552 343 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 2040 3349 4143 4163 2040 Size: 1309 794 20 3710 Fragments arranged by size: 3710 1309 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2828 2863 529 Size: 35 2264 35 3499 Fragments arranged by size: 3499 2264 35 35 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DraIII CAC_nnn'GTG Cuts at: 2999 2999 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798 Size: 54 1425 50 275 18 1450 889 1629 Cuts at: 5798 8 Size: 43 Fragments arranged by size: 1629 1450 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2053 2204 2997 3763 1353 Size: 700 151 793 766 3423 Fragments arranged by size: 3423 793 766 700 151 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2202 2678 3896 4493 5280 5548 1239 Size: 393 570 476 1218 597 787 268 1524 Fragments arranged by size: 1524 1218 787 597 570 476 393 268 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2220 2220 Size: 5833 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2373 2405 4474 4506 4548 Size: 32 135 32 801 32 2069 32 42 Cuts at: 4548 4580 1373 Size: 32 2626 Fragments arranged by size: 2626 2069 801 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2740 4947 5131 1305 Size: 1435 2207 184 2007 Fragments arranged by size: 2207 2007 1435 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4011 Fragments arranged by size: 4011 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2561 3791 4150 4309 518 Size: 648 1395 1230 359 159 2042 Fragments arranged by size: 2042 1395 1230 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3249 3926 5074 5319 1606 Size: 1643 677 1148 245 2120 Fragments arranged by size: 2120 1643 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2872 513 Size: 552 1807 3474 Fragments arranged by size: 3474 1807 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NsiI A_TGCA'T Cuts at: 2010 2260 2010 Size: 250 5583 NspI r_CATG'y Cuts at: 1132 1229 2262 2883 3191 4736 1132 Size: 97 1033 621 308 1545 2229 Fragments arranged by size: 2229 1545 1033 621 308 97 PacI TTA_AT'TAA Cuts at: 2505 2505 Size: 5833 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 4732 4732 Size: 5833 PflMI CCAn_nnn'nTGG Cuts at: 1108 2408 1108 Size: 1300 4533 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3902 3934 5415 5447 3902 Size: 32 1481 32 4288 Fragments arranged by size: 4288 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2183 1613 Size: 570 5263 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 3034 3185 830 Size: 2204 151 3478 Fragments arranged by size: 3478 2204 151 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3034 3185 3034 Size: 151 5682 ScaI AGT'ACT Cuts at: 2294 4081 5793 2294 Size: 1787 1712 2334 Fragments arranged by size: 2334 1787 1712 SexAI A'CCwGG_T Cuts at: 2415 2415 Size: 5833 SfcI C'TryA_G Cuts at: 826 1045 3030 3181 4316 4997 5188 826 Size: 219 1985 151 1135 681 191 1471 Fragments arranged by size: 1985 1471 1135 681 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2015 3937 4838 5100 820 Size: 197 926 72 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 262 197 72 SnaBI TAC'GTA Cuts at: 493 3624 493 Size: 3131 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 3757 5 Size: 47 3705 2081 Fragments arranged by size: 3705 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 2872 3272 513 Size: 552 1807 400 3074 Fragments arranged by size: 3074 1807 552 400 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3860 3877 4030 4215 4660 325 Size: 1255 2280 17 153 185 445 1498 Fragments arranged by size: 2280 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 4079 5791 Size: 274 80 33 51 1443 315 1787 1712 Cuts at: 5791 96 Size: 138 Fragments arranged by size: 1787 1712 1443 315 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2214 2246 5793 5825 2214 Size: 32 3547 32 2222 Fragments arranged by size: 3547 2222 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5833 XbaI T'CTAG_A Cuts at: 3169 3169 Size: 5833 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3117 3962 3117 Size: 845 4988 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI