################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaHI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau96I SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AclI 391 AACGTT AA^CGTT AcsI 485 AAATTT [GA]^AATT[CT] AlwNI 398 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 485 AAATTT [GA]^AATT[CT] Asp700I 1108 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BclI 380 TGATCA T^GATCA BfaI 992 CTAG C^TAG BglII 684 AGATCT A^GATCT BpmI 176 CTGGAG CTGGAG(16/14) BsaBI 1111 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 659 ACCGGA [AT]^CCGG[AT] BsmFI 871 GGGAC GGGAC(10/14) BstYI 684 AGATCT [GA]^GATC[CT] Cac8I 303 GCTTGC GC[ACGT]^[ACGT]GC DraIII 989 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 867 CCATGG C^C[AT][AT]GG FbaI 380 TGATCA T^GATCA HapII 660 CCGG C^CGG HpaII 660 CCGG C^CGG MaeI 992 CTAG C^TAG MaeII 392 ACGT A^CGT MamI 1111 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 684 AGATCT [GA]^GATC[CT] MslI 552 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 660 CCGG C^CGG NcoI 867 CCATGG C^CATGG PacI 496 TTAATTAA TTAAT^TAA PflMI 397 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 179 GAGTC GAGTC(4/5) PshAI 174 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 391 AACGTT AA^CGTT PstI 1025 CTGCAG CTGCA^G SbfI 1024 CCTGCAGG CCTGCA^GG ScaI 287 AGTACT AGT^ACT SexAI 410 ACCAGGT A^CC[AT]GGT SfaNI 3 GCATC GCATC(5/9) SfcI 1025 CTGCAG C^T[GA][CT]AG SmlI 10 CTCAAG C^T[CT][GA]AG Sse8387I 1024 CCTGCAGG CCTGCA^GG StyI 867 CCATGG C^C[AT][AT]GG TaqI 717 TCGA T^CGA Tsp45I 589 GTCAC ^GT[GC]AC TthHB8I 717 TCGA T^CGA Van91I 397 CCAGGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 684 AGATCT [GA]^GATC[CT] XmnI 1108 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 193 AGCT AG^CT 763 AGCT BbvI 669 GCAGC GCAGC(8/12) 764 GCTGC BsaI 295 GAGACC GGTCTC(1/5) 573 GGTCTC BseRI 1000 GAGGAG GAGGAG(10/8) 1057 GAGGAG DsaI 524 CCGTGG C^C[GA][CT]GG 867 CCATGG EcoT22I 1 ATGCAT ATGCA^T 251 ATGCAT Fnu4HI 669 GCAGC GC^[ACGT]GC 764 GCTGC HphI 988 TCACC GGTGA(8/7) 1123 TCACC ItaI 669 GCAGC GC^[ACGT]GC 764 GCTGC MaeIII 467 GTAAC ^GT[ACGT]AC 589 GTCAC NlaIV 367 GGAGCC GG[ACGT]^[ACGT]CC 1030 GGAACC NsiI 1 ATGCAT ATGCA^T 251 ATGCAT NspI 253 GCATGT [GA]CATG^[CT] 874 ACATGC TseI 669 GCAGC G^C[AT]GC 764 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI DdeI DpnII EarI EcoRII FokI HinfI Ksp632I MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI