SCUBE2 ORF clone pFN21AE2478

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21AE2478

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Clone info.

Species

human

Clone Name

pFN21AE2478 Sequence (with vector) Restriction Map

Product ID

FHC24148

ORF Sequence

DNA sequence (2913 bp)
Protein sequence (971 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2478

Symbol

SCUBE2

Alias : CEGB1, CEGF1, CEGP1, scube/You

Description

signal peptide, CUB domain, EGF-like 2, transcript variant 1

Original Clone ID

cp01621

Note

We obtained this clone using the E.coli strain CopyCutter EPI400.Firstly we obtained it using DH10B at 25 deg C.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2913 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 971 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7797.2 0 100.0 SCUBE2 CCDS81553.1 2.8e-132 91.6 SCUBE2 CCDS53599.1 1.6e-121 72.9 SCUBE2 CCDS4800.1 2.7e-80 56.3 SCUBE3 CCDS14048.1 1.2e-67 62.3 SCUBE1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR013091 45 84 PF07645 EGF calcium-binding IPR013091 128 167 PF07645 EGF calcium-binding IPR013091 323 362 PF07645 EGF calcium-binding IPR013091 364 394 PF07645 EGF calcium-binding IPR013091 403 442 PF07645 EGF calcium-binding IPR011641 673 720 PF07699 Tyrosine-protein kinase ephrin type A/B receptor-like IPR011641 729 776 PF07699 Tyrosine-protein kinase ephrin type A/B receptor-like IPR000859 781 890 PF00431 CUB HMMSmart IPR001881 45 85 SM00179 EGF-like calcium-binding IPR006210 48 85 SM00181 Epidermal growth factor-like IPR001881 86 127 SM00179 EGF-like calcium-binding IPR006210 89 127 SM00181 Epidermal growth factor-like IPR001881 128 168 SM00179 EGF-like calcium-binding IPR006210 131 168 SM00181 Epidermal growth factor-like IPR006210 176 214 SM00181 Epidermal growth factor-like IPR001881 209 253 SM00179 EGF-like calcium-binding IPR006210 216 253 SM00181 Epidermal growth factor-like IPR006210 285 322 SM00181 Epidermal growth factor-like IPR001881 286 322 SM00179 EGF-like calcium-binding IPR001881 323 363 SM00179 EGF-like calcium-binding IPR006210 326 363 SM00181 Epidermal growth factor-like IPR001881 364 402 SM00179 EGF-like calcium-binding IPR006210 367 402 SM00181 Epidermal growth factor-like IPR001881 403 443 SM00179 EGF-like calcium-binding IPR006210 406 443 SM00181 Epidermal growth factor-like IPR006210 719 757 SM00181 Epidermal growth factor-like IPR000859 781 893 SM00042 CUB ProfileScan IPR000742 45 85 PS50026 Epidermal growth factor-like IPR000742 86 127 PS50026 Epidermal growth factor-like IPR000742 364 402 PS50026 Epidermal growth factor-like IPR000742 403 443 PS50026 Epidermal growth factor-like IPR000859 781 893 PS01180 CUB
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 12 AWAVLLLLLLLPPLLLLAGAVPP 34 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_066025 971 611747 100.0 100.0 XP_005253092 953 611747 93.9 92.2 XP_011518548 962 611747 87.9 100.0 XP_005253089 1028 611747 94.3 97.4 NP_001317128 999 611747 91.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2478 Download

>KIEE2478  2913 bp
ATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGCCTGGGCGGTGCTGCTGCTGCTGCTG
CTGCTGCCGCCACTGCTGCTGCTGGCGGGGGCCGTCCCGCCGGGTCGGGGCCGTGCCGCG
GGGCCGCAGGAGGATGTAGATGAGTGTGCCCAAGGCCTAGATGACTGCCATGCCGACGCC
CTGTGTCAGAACACACCCACCTCCTACAAGTGCTCCTGCAAGCCTGGCTACCAAGGGGAA
GGCAGGCAGTGTGAGGACATCGATGAATGTGGAAATGAGCTCAATGGAGGCTGTGTCCAT
GACTGTTTGAATATTCCAGGCAATTATCGTTGCACTTGTTTTGATGGCTTCATGTTGGCT
CATGACGGTCATAATTGTCTTGATGTGGACGAGTGCCTGGAGAACAATGGCGGCTGCCAG
CATACCTGTGTCAACGTCATGGGGAGCTATGAGTGCTGCTGCAAGGAGGGGTTTTTCCTG
AGTGACAATCAGCACACCTGCATTCACCGCTCGGAAGAGGGCCTGAGCTGCATGAATAAG
GATCACGGCTGTAGTCACATCTGCAAGGAGGCCCCAAGGGGCAGCGTCGCCTGTGAGTGC
AGGCCTGGTTTTGAGCTGGCCAAGAACCAGAGAGACTGCATCTTGACCTGTAACCATGGG
AACGGTGGGTGCCAGCACTCCTGTGACGATACAGCCGATGGCCCAGAGTGCAGCTGCCAT
CCACAGTACAAGATGCACACAGATGGGAGGAGCTGCCTTGAGCGAGAGGACACTGTCCTG
GAGGTGACAGAGAGCAACACCACATCAGTGGTGGATGGGGATAAACGGGTGAAACGGCGG
CTGCTCATGGAAACGTGTGCTGTCAACAATGGAGGCTGTGACCGCACCTGTAAGGATACT
TCGACAGGTGTCCACTGCAGTTGTCCTGTTGGATTCACTCTCCAGTTGGATGGGAAGACA
TGTAAAGATATTGATGAGTGCCAGACCCGCAATGGAGGTTGTGATCATTTCTGCAAAAAC
ATCGTGGGCAGTTTTGACTGCGGCTGCAAGAAAGGATTTAAATTATTAACAGATGAGAAG
TCTTGCCAAGATGTGGATGAGTGCTCTTTGGATAGGACCTGTGACCACAGCTGCATCAAC
CACCCTGGCACATTTGCTTGTGCTTGCAACCGAGGGTACACCCTGTATGGCTTCACCCAC
TGTGGAGACACCAATGAGTGCAGCATCAACAACGGAGGCTGTCAGCAGGTCTGTGTGAAC
ACAGTGGGCAGCTATGAATGCCAGTGCCACCCTGGGTACAAGCTCCACTGGAATAAAAAA
GACTGTGTGGAAGTGAAGGGGCTCCTGCCCACAAGTGTGTCACCCCGTGTGTCCCTGCAC
TGCGGTAAGAGTGGTGGAGGAGACGGGTGCTTCCTCAGATGTCACTCTGGCATTCACCTC
TCTTCAGGACTGCAAGGGGCCTACTCTGTCACCTGTGGCTCTTCCTCTCCTCTCAGGAAC
AAACAACAAAAATCAAATGACTCTGCTTTTGGGGATGTCACCACCATCAGGACAAGTGTA
ACCTTTAAGCTAAATGAAGGCAAGTGTAGTTTGAAAAATGCTGAGCTGTTTCCCGAGGGT
CTGCGACCAGCACTACCAGAGAAGCACAGCTCAGTAAAAGAGAGCTTCCGCTACGTAAAC
CTTACATGCAGCTCTGGCAAGCAAGTCCCAGGAGCCCCTGGCCGACCAAGCACCCCTAAG
GAAATGTTTATCACTGTTGAGTTTGAGCTTGAAACTAACCAAAAGGAGGTGACAGCTTCT
TGTGACCTGAGCTGCATCGTAAAGCGAACCGAGAAGCGGCTCCGTAAAGCCATCCGCACG
CTCAGAAAGGCCGTCCACAGGGAGCAGTTTCACCTCCAGCTCTCAGGCATGAACCTCGAC
GTGGCTAAAAAGCCTCCCAGAACATCTGAACGCCAGGCAGAGTCCTGTGGAGTGGGCCAG
GGTCATGCAGAAAACCAATGTGGTCTGTGTCAACCTGGTGAATATTCTGCAGATGGCTTT
GCACCTTGCCAGCTCTGTGCCCTGGGCACGTTCCAGCCTGAAGCTGGTCGAACTTCCTGC
TTCCCCTGTGGAGGAGGCCTTGCCACCAAACATCAGGGAGCTACTTCCTTTCAGGACTGT
GAAACCAGAGTTCAATGTTCACCTGGACATTTCTACAACACCACCACTCACCGATGTATT
CGTTGCCCAGTGGGAACATACCAGCCTGAATTTGGAAAAAATAATTGTGTTTCTTGCCCA
GGAAATACTACGACTGACTTTGATGGCTCCACAAACATAACCCAGTGTAAAAACAGAAGA
TGTGGAGGGGAGCTGGGAGATTTCACTGGGTACATTGAATCCCCAAACTACCCAGGCAAT
TACCCAGCCAACACCGAGTGTACGTGGACCATCAACCCACCCCCCAAGCGCCGCATCCTG
ATCGTGGTCCCTGAGATCTTCCTGCCCATAGAGGACGACTGTGGGGACTATCTGGTGATG
CGGAAAACCTCTTCATCCAATTCTGTGACAACATATGAAACCTGCCAGACCTACGAACGC
CCCATCGCCTTCACCTCCAGGTCAAAGAAGCTGTGGATTCAGTTCAAGTCCAATGAAGGG
AACAGCGCTAGAGGGTTCCAGGTCCCATACGTGACATATGATGAGGACTACCAGGAACTC
ATTGAAGACATAGTTCGAGATGGCAGGCTCTATGCATCTGAGAACCATCAGGAAATACTT
AAGGATAAGAAACTTATCAAGGCTCTGTTTGATGTCCTGGCCCATCCCCAGAACTATTTC
AAGTACACAGCCCAGGAGTCCCGAGAGATGTTTCCAAGATCGTTCATCCGATTGCTACGT
TCCAAAGTGTCCAGGTTTTTGAGACCTTACAAA

ORF Protein

Cloned ORF protein sequence for pFN21AE2478 Download

>KIEE2478  971 aa
MGVAGRNRPGAAWAVLLLLLLLPPLLLLAGAVPPGRGRAAGPQEDVDECAQGLDDCHADA
LCQNTPTSYKCSCKPGYQGEGRQCEDIDECGNELNGGCVHDCLNIPGNYRCTCFDGFMLA
HDGHNCLDVDECLENNGGCQHTCVNVMGSYECCCKEGFFLSDNQHTCIHRSEEGLSCMNK
DHGCSHICKEAPRGSVACECRPGFELAKNQRDCILTCNHGNGGCQHSCDDTADGPECSCH
PQYKMHTDGRSCLEREDTVLEVTESNTTSVVDGDKRVKRRLLMETCAVNNGGCDRTCKDT
STGVHCSCPVGFTLQLDGKTCKDIDECQTRNGGCDHFCKNIVGSFDCGCKKGFKLLTDEK
SCQDVDECSLDRTCDHSCINHPGTFACACNRGYTLYGFTHCGDTNECSINNGGCQQVCVN
TVGSYECQCHPGYKLHWNKKDCVEVKGLLPTSVSPRVSLHCGKSGGGDGCFLRCHSGIHL
SSGLQGAYSVTCGSSSPLRNKQQKSNDSAFGDVTTIRTSVTFKLNEGKCSLKNAELFPEG
LRPALPEKHSSVKESFRYVNLTCSSGKQVPGAPGRPSTPKEMFITVEFELETNQKEVTAS
CDLSCIVKRTEKRLRKAIRTLRKAVHREQFHLQLSGMNLDVAKKPPRTSERQAESCGVGQ
GHAENQCGLCQPGEYSADGFAPCQLCALGTFQPEAGRTSCFPCGGGLATKHQGATSFQDC
ETRVQCSPGHFYNTTTHRCIRCPVGTYQPEFGKNNCVSCPGNTTTDFDGSTNITQCKNRR
CGGELGDFTGYIESPNYPGNYPANTECTWTINPPPKRRILIVVPEIFLPIEDDCGDYLVM
RKTSSSNSVTTYETCQTYERPIAFTSRSKKLWIQFKSNEGNSARGFQVPYVTYDEDYQEL
IEDIVRDGRLYASENHQEILKDKKLIKALFDVLAHPQNYFKYTAQESREMFPRSFIRLLR
SKVSRFLRPYK

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2478 Download

>pFN21AE2478  7618 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGC
CTGGGCGGTGCTGCTGCTGCTGCTGCTGCTGCCGCCACTGCTGCTGCTGGCGGGGGCCGT
CCCGCCGGGTCGGGGCCGTGCCGCGGGGCCGCAGGAGGATGTAGATGAGTGTGCCCAAGG
CCTAGATGACTGCCATGCCGACGCCCTGTGTCAGAACACACCCACCTCCTACAAGTGCTC
CTGCAAGCCTGGCTACCAAGGGGAAGGCAGGCAGTGTGAGGACATCGATGAATGTGGAAA
TGAGCTCAATGGAGGCTGTGTCCATGACTGTTTGAATATTCCAGGCAATTATCGTTGCAC
TTGTTTTGATGGCTTCATGTTGGCTCATGACGGTCATAATTGTCTTGATGTGGACGAGTG
CCTGGAGAACAATGGCGGCTGCCAGCATACCTGTGTCAACGTCATGGGGAGCTATGAGTG
CTGCTGCAAGGAGGGGTTTTTCCTGAGTGACAATCAGCACACCTGCATTCACCGCTCGGA
AGAGGGCCTGAGCTGCATGAATAAGGATCACGGCTGTAGTCACATCTGCAAGGAGGCCCC
AAGGGGCAGCGTCGCCTGTGAGTGCAGGCCTGGTTTTGAGCTGGCCAAGAACCAGAGAGA
CTGCATCTTGACCTGTAACCATGGGAACGGTGGGTGCCAGCACTCCTGTGACGATACAGC
CGATGGCCCAGAGTGCAGCTGCCATCCACAGTACAAGATGCACACAGATGGGAGGAGCTG
CCTTGAGCGAGAGGACACTGTCCTGGAGGTGACAGAGAGCAACACCACATCAGTGGTGGA
TGGGGATAAACGGGTGAAACGGCGGCTGCTCATGGAAACGTGTGCTGTCAACAATGGAGG
CTGTGACCGCACCTGTAAGGATACTTCGACAGGTGTCCACTGCAGTTGTCCTGTTGGATT
CACTCTCCAGTTGGATGGGAAGACATGTAAAGATATTGATGAGTGCCAGACCCGCAATGG
AGGTTGTGATCATTTCTGCAAAAACATCGTGGGCAGTTTTGACTGCGGCTGCAAGAAAGG
ATTTAAATTATTAACAGATGAGAAGTCTTGCCAAGATGTGGATGAGTGCTCTTTGGATAG
GACCTGTGACCACAGCTGCATCAACCACCCTGGCACATTTGCTTGTGCTTGCAACCGAGG
GTACACCCTGTATGGCTTCACCCACTGTGGAGACACCAATGAGTGCAGCATCAACAACGG
AGGCTGTCAGCAGGTCTGTGTGAACACAGTGGGCAGCTATGAATGCCAGTGCCACCCTGG
GTACAAGCTCCACTGGAATAAAAAAGACTGTGTGGAAGTGAAGGGGCTCCTGCCCACAAG
TGTGTCACCCCGTGTGTCCCTGCACTGCGGTAAGAGTGGTGGAGGAGACGGGTGCTTCCT
CAGATGTCACTCTGGCATTCACCTCTCTTCAGGACTGCAAGGGGCCTACTCTGTCACCTG
TGGCTCTTCCTCTCCTCTCAGGAACAAACAACAAAAATCAAATGACTCTGCTTTTGGGGA
TGTCACCACCATCAGGACAAGTGTAACCTTTAAGCTAAATGAAGGCAAGTGTAGTTTGAA
AAATGCTGAGCTGTTTCCCGAGGGTCTGCGACCAGCACTACCAGAGAAGCACAGCTCAGT
AAAAGAGAGCTTCCGCTACGTAAACCTTACATGCAGCTCTGGCAAGCAAGTCCCAGGAGC
CCCTGGCCGACCAAGCACCCCTAAGGAAATGTTTATCACTGTTGAGTTTGAGCTTGAAAC
TAACCAAAAGGAGGTGACAGCTTCTTGTGACCTGAGCTGCATCGTAAAGCGAACCGAGAA
GCGGCTCCGTAAAGCCATCCGCACGCTCAGAAAGGCCGTCCACAGGGAGCAGTTTCACCT
CCAGCTCTCAGGCATGAACCTCGACGTGGCTAAAAAGCCTCCCAGAACATCTGAACGCCA
GGCAGAGTCCTGTGGAGTGGGCCAGGGTCATGCAGAAAACCAATGTGGTCTGTGTCAACC
TGGTGAATATTCTGCAGATGGCTTTGCACCTTGCCAGCTCTGTGCCCTGGGCACGTTCCA
GCCTGAAGCTGGTCGAACTTCCTGCTTCCCCTGTGGAGGAGGCCTTGCCACCAAACATCA
GGGAGCTACTTCCTTTCAGGACTGTGAAACCAGAGTTCAATGTTCACCTGGACATTTCTA
CAACACCACCACTCACCGATGTATTCGTTGCCCAGTGGGAACATACCAGCCTGAATTTGG
AAAAAATAATTGTGTTTCTTGCCCAGGAAATACTACGACTGACTTTGATGGCTCCACAAA
CATAACCCAGTGTAAAAACAGAAGATGTGGAGGGGAGCTGGGAGATTTCACTGGGTACAT
TGAATCCCCAAACTACCCAGGCAATTACCCAGCCAACACCGAGTGTACGTGGACCATCAA
CCCACCCCCCAAGCGCCGCATCCTGATCGTGGTCCCTGAGATCTTCCTGCCCATAGAGGA
CGACTGTGGGGACTATCTGGTGATGCGGAAAACCTCTTCATCCAATTCTGTGACAACATA
TGAAACCTGCCAGACCTACGAACGCCCCATCGCCTTCACCTCCAGGTCAAAGAAGCTGTG
GATTCAGTTCAAGTCCAATGAAGGGAACAGCGCTAGAGGGTTCCAGGTCCCATACGTGAC
ATATGATGAGGACTACCAGGAACTCATTGAAGACATAGTTCGAGATGGCAGGCTCTATGC
ATCTGAGAACCATCAGGAAATACTTAAGGATAAGAAACTTATCAAGGCTCTGTTTGATGT
CCTGGCCCATCCCCAGAACTATTTCAAGTACACAGCCCAGGAGTCCCGAGAGATGTTTCC
AAGATCGTTCATCCGATTGCTACGTTCCAAAGTGTCCAGGTTTTTGAGACCTTACAAAGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT