Restriction map for
pFN21AE2478
(Linear) (Six-base) MAPSORT of: KIEE2478.seq Check: 8736 from: 1 to: 2913
KIEE2478.orf
With 182 enzymes: SgfI *
July 26, 2013 11:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 505 2913
Size: 505 2408
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 2099 2913
Size: 1427 672 814
Fragments arranged by size:
1427 814 672
AfeI AGC'GCT
Cuts at: 0 2646 2913
Size: 2646 267
AflII C'TTAA_G
Cuts at: 0 2758 2913
Size: 2758 155
AflIII A'CryG_T
Cuts at: 0 853 958 2913
Size: 853 105 1955
Fragments arranged by size:
1955 853 105
AleI CACnn'nnGTG
Cuts at: 0 680 805 1354 2913
Size: 680 125 549 1559
Fragments arranged by size:
1559 680 549 125
ApoI r'AATT_y
Cuts at: 0 2248 2913
Size: 2248 665
AvaI C'yCGr_G
Cuts at: 0 25 1612 2840 2913
Size: 25 1587 1228 73
Fragments arranged by size:
1587 1228 73 25
BanI G'GyrC_C
Cuts at: 0 668 2913
Size: 668 2245
BanII G_rGCy'C
Cuts at: 0 281 1343 1716 2913
Size: 281 1062 373 1197
Fragments arranged by size:
1197 1062 373 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 961 2711 2913
Size: 961 1750 202
Fragments arranged by size:
1750 961 202
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1002 1036 1896 1930 2078 2112 2442 2476
Size: 1002 34 860 34 148 34 330 34
Cuts at: 2476 2913
Size: 437
Fragments arranged by size:
1002 860 437 330 148 34 34 34
34
BciVI GTATCCnnnnn_n'
Cuts at: 0 888 2913
Size: 888 2025
BclI T'GATC_A
Cuts at: 0 1002 2913
Size: 1002 1911
BfrBI ATG'CAT
Cuts at: 0 2734 2913
Size: 2734 179
BglII A'GATC_T
Cuts at: 0 2474 2913
Size: 2474 439
BlpI GC'TnA_GC
Cuts at: 0 1601 2913
Size: 1601 1312
Bme1580I G_kGCm'C
Cuts at: 0 150 2061 2068 2913
Size: 150 1911 7 845
Fragments arranged by size:
1911 845 150 7
BmgBI CAC'GTC
Cuts at: 0 1920 2913
Size: 1920 993
BmrI ACTGGGnnnn_n'
Cuts at: 0 2221 2316 2375 2913
Size: 2221 95 59 538
Fragments arranged by size:
2221 538 95 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 799 925 1879 2580 2913
Size: 418 381 126 954 701 333
Fragments arranged by size:
954 701 418 381 333 126
Bpu10I CC'TnA_GC
Cuts at: 0 523 1807 2913
Size: 523 1284 1106
Fragments arranged by size:
1284 1106 523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 2913
Size: 778 2135
BsaI GGTCTCn'nnnn_
Cuts at: 0 2895 2913
Size: 2895 18
BsaAI yAC'GTr
Cuts at: 0 1674 2423 2670 2913
Size: 1674 749 247 243
Fragments arranged by size:
1674 749 247 243
BsaHI Gr'CG_yC
Cuts at: 0 176 2913
Size: 176 2737
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 278 308 2913
Size: 196 30 52 30 2605
Fragments arranged by size:
2605 196 52 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 762 1412 1478 2126 2913
Size: 762 650 66 648 787
Fragments arranged by size:
787 762 650 648 66
BseYI C'CCAG_C
Cuts at: 0 2352 2403 2913
Size: 2352 51 510
Fragments arranged by size:
2352 510 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 729 1239 1360 2913
Size: 618 111 510 121 1553
Fragments arranged by size:
1553 618 510 121 111
BsiHKAI G_wGCw'C
Cuts at: 0 214 281 1105 2913
Size: 214 67 824 1808
Fragments arranged by size:
1808 824 214 67
BsmI GAATG_Cn'
Cuts at: 0 500 1282 1430 2913
Size: 500 782 148 1483
Fragments arranged by size:
1483 782 500 148
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1394 2913
Size: 1394 1519
Bsp1286I G_dGCh'C
Cuts at: 0 150 214 281 1105 1343 1716 2061 2068
Size: 150 64 67 824 238 373 345 7
Cuts at: 2068 2913
Size: 845
Fragments arranged by size:
845 824 373 345 238 150 67 64
7
BspHI T'CATG_A
Cuts at: 0 360 2913
Size: 360 2553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 505 1236 2569 2913
Size: 505 731 1333 344
Fragments arranged by size:
1333 731 505 344
BsrBI CCG'CTC
Cuts at: 0 509 2913
Size: 509 2404
BsrDI GCAATG_nn'
Cuts at: 0 996 2913
Size: 996 1917
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 949 2913
Size: 807 142 1964
Fragments arranged by size:
1964 807 142
BstYI r'GATC_y
Cuts at: 0 2474 2913
Size: 2474 439
Bsu36I CC'TnA_GG
Cuts at: 0 1736 2913
Size: 1736 1177
BtgI C'CryG_G
Cuts at: 0 116 654 2913
Size: 116 538 2259
Fragments arranged by size:
2259 538 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2568 2913
Size: 2568 345
BtsI GCAGTG_nn'
Cuts at: 0 70 253 912 1377 2913
Size: 70 183 659 465 1536
Fragments arranged by size:
1536 659 465 183 70
ClaI AT'CG_AT
Cuts at: 0 260 2913
Size: 260 2653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 901 936 2913
Size: 901 35 1977
Fragments arranged by size:
1977 901 35
DraI TTT'AAA
Cuts at: 0 1059 2913
Size: 1059 1854
DraIII CAC_nnn'GTG
Cuts at: 0 1366 2417 2913
Size: 1366 1051 496
Fragments arranged by size:
1366 1051 496
EaeI y'GGCC_r
Cuts at: 0 617 1719 2913
Size: 617 1102 1194
Fragments arranged by size:
1194 1102 617
EarI CTCTTCn'nnn_
Cuts at: 0 509 1446 1485 2535 2913
Size: 509 937 39 1050 378
Fragments arranged by size:
1050 937 509 378 39
EcoICRI GAG'CTC
Cuts at: 0 279 2913
Size: 279 2634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 799 925 1427 1879 2099 2580 2913
Size: 418 381 126 502 452 220 481 333
Fragments arranged by size:
502 481 452 418 381 333 220 126
EcoO109I rG'GnC_Cy
Cuts at: 0 519 570 1115 1457 2661 2913
Size: 519 51 545 342 1204 252
Fragments arranged by size:
1204 545 519 342 252 51
HaeII r_GCGC'y
Cuts at: 0 2451 2648 2913
Size: 2451 197 265
Fragments arranged by size:
2451 265 197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 278 310 471 503 623 655 923 955
Size: 278 32 161 32 120 32 268 32
Cuts at: 955 1063 1087 1095 1119 2849 2881 2913
Size: 108 24 8 24 1730 32 32
Fragments arranged by size:
1730 278 268 161 120 108 32 32
32 32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 0 432 864 2011 2913
Size: 432 432 1147 902
Fragments arranged by size:
1147 902 432 432
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 909 926 2913
Size: 909 17 1987
Fragments arranged by size:
1987 909 17
MscI TGG'CCA
Cuts at: 0 619 2913
Size: 619 2294
MslI CAynn'nnrTG
Cuts at: 0 425 680 740 805 1354 2913
Size: 425 255 60 65 549 1559
Fragments arranged by size:
1559 549 425 255 65 60
MspA1I CmG'CkG
Cuts at: 0 118 713 1130 2913
Size: 118 595 417 1783
Fragments arranged by size:
1783 595 417 118
NcoI C'CATG_G
Cuts at: 0 654 2913
Size: 654 2259
NdeI CA'TA_TG
Cuts at: 0 2553 2676 2913
Size: 2553 123 237
Fragments arranged by size:
2553 237 123
NsiI A_TGCA'T
Cuts at: 0 2736 2913
Size: 2736 177
NspI r_CATG'y
Cuts at: 0 962 1688 2913
Size: 962 726 1225
Fragments arranged by size:
1225 962 726
PasI CC'CwG_GG
Cuts at: 0 1291 2061 2913
Size: 1291 770 852
Fragments arranged by size:
1291 852 770
PciI A'CATG_T
Cuts at: 0 958 2913
Size: 958 1955
PflMI CCAn_nnn'nTGG
Cuts at: 0 1092 2913
Size: 1092 1821
PfoI T'CCnGG_A
Cuts at: 0 776 2913
Size: 776 2137
PpuMI rG'GwC_Cy
Cuts at: 0 1115 2661 2913
Size: 1115 1546 252
Fragments arranged by size:
1546 1115 252
PshAI GACnn'nnGTC
Cuts at: 0 907 2913
Size: 907 2006
PstI C_TGCA'G
Cuts at: 0 919 2031 2913
Size: 919 1112 882
Fragments arranged by size:
1112 919 882
PvuII CAG'CTG
Cuts at: 0 713 1130 2913
Size: 713 417 1783
Fragments arranged by size:
1783 713 417
SacI G_AGCT'C
Cuts at: 0 281 2913
Size: 281 2632
SacII CC_GC'GG
Cuts at: 0 119 2913
Size: 119 2794
SapI GCTCTTCn'nnn_
Cuts at: 0 1485 2913
Size: 1485 1428
SexAI A'CCwGG_T
Cuts at: 0 2013 2913
Size: 2013 900
SfcI C'TryA_G
Cuts at: 0 549 915 2027 2913
Size: 549 366 1112 886
Fragments arranged by size:
1112 886 549 366
SmaI CCC'GGG
Cuts at: 0 27 2913
Size: 27 2886
SmlI C'TyrA_G
Cuts at: 0 757 2758 2913
Size: 757 2001 155
Fragments arranged by size:
2001 757 155
SnaBI TAC'GTA
Cuts at: 0 1674 2913
Size: 1674 1239
SspI AAT'ATT
Cuts at: 0 312 2023 2913
Size: 312 1711 890
Fragments arranged by size:
1711 890 312
StuI AGG'CCT
Cuts at: 0 155 603 2117 2913
Size: 155 448 1514 796
Fragments arranged by size:
1514 796 448 155
StyI C'CwwG_G
Cuts at: 0 150 231 574 654 2913
Size: 150 81 343 80 2259
Fragments arranged by size:
2259 343 150 81 80
SwaI ATTT'AAAT
Cuts at: 0 1059 2913
Size: 1059 1854
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 210 656 1210 2913
Size: 210 446 554 1703
Fragments arranged by size:
1703 554 446 210
TatI w'GTAC_w
Cuts at: 0 725 2822 2913
Size: 725 2097 91
Fragments arranged by size:
2097 725 91
Tth111I GACn'n_nGTC
Cuts at: 0 772 2913
Size: 772 2141
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 661 807 988 1288 2080 2248 2913
Size: 661 146 181 300 792 168 665
Fragments arranged by size:
792 665 661 300 181 168 146
XmaI C'CCGG_G
Cuts at: 0 25 2913
Size: 25 2888
XmnI GAAnn'nnTTC
Cuts at: 0 2815 2913
Size: 2815 98
Enzymes that do cut:
AarI AcuI AfeI AflII AflIII AleI ApoI AvaI
BanI BanII BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PasI PciI PflMI PfoI PpuMI PshAI PstI PvuII
SacI SacII SapI SexAI SfcI SmaI SmlI SnaBI
SspI StuI StyI SwaI TaqII TatI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AgeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BglI BmtI BplI BsaBI BsaWI
BsiEI BsiWI BspEI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I DrdI EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SpeI SphI SrfI TstI XbaI
XhoI ZraI