################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI AhdI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbvCI BglI BlnI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspEI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Cfr10I CpoI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV FseI FspI HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcsI 2248 GAATTT [GA]^AATT[CT] AcyI 175 GACGCC G[GA]^CG[CT]C AflII 2758 CTTAAG C^TTAAG AlwI 540 GGATC GGATC(4/5) AocI 1735 CCTAAGG CC^T[ACGT]AGG Aor51HI 2644 AGCGCT AGC^GCT ApoI 2248 GAATTT [GA]^AATT[CT] Asp700I 2811 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 769 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 617 TGGCCA TGG^CCA BanI 668 GGTGCC G^G[CT][GA]CC BciVI 894 GGATAC GTATCC(6/5) BclI 1002 TGATCA T^GATCA BfrI 2758 CTTAAG C^TTAAG BglII 2474 AGATCT A^GATCT BlpI 1600 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1600 GCTGAGC GC^T[ACGT]AGC BsaHI 175 GACGCC G[GA]^CG[CT]C BsaI 2901 GAGACC GGTCTC(1/5) BsmBI 1400 GAGACG CGTCTC(1/5) BspDI 259 ATCGAT AT^CGAT BspHI 360 TCATGA T^CATGA BspLU11I 958 ACATGT A^CATGT BsrBI 507 CCGCTC CCGCTC(-3/-3) BsrDI 989 GCAATG GCAATG(2/0) BstYI 2474 AGATCT [GA]^GATC[CT] Bsu36I 1735 CCTAAGG CC^T[ACGT]AGG CelII 1600 GCTGAGC GC^T[ACGT]AGC ClaI 259 ATCGAT AT^CGAT DraI 1057 TTTAAA TTT^AAA Eco47III 2644 AGCGCT AGC^GCT Eco81I 1735 CCTAAGG CC^T[ACGT]AGG EcoT22I 2732 ATGCAT ATGCA^T FbaI 1002 TGATCA T^GATCA Hin1I 175 GACGCC G[GA]^CG[CT]C KspI 116 CCGCGG CCGC^GG MflI 2474 AGATCT [GA]^GATC[CT] MluNI 617 TGGCCA TGG^CCA MscI 617 TGGCCA TGG^CCA NcoI 654 CCATGG C^CATGG NsiI 2732 ATGCAT ATGCA^T PflFI 769 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1086 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 903 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 360 TCATGA T^CATGA SacI 277 GAGCTC GAGCT^C SacII 116 CCGCGG CCGC^GG SapI 1478 GCTCTTC GCTCTTC(1/4) SexAI 2013 ACCTGGT A^CC[AT]GGT SmaI 25 CCCGGG CCC^GGG SnaBI 1672 TACGTA TAC^GTA SwaI 1056 ATTTAAAT ATTT^AAAT Tth111I 769 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1086 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 2474 AGATCT [GA]^GATC[CT] XmaCI 25 CCCGGG C^CCGGG XmaI 25 CCCGGG C^CCGGG XmnI 2811 GAACTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccII 9 CGCG CG^CG 117 CGCG AflIII 853 ACGTGT A^C[GA][CT]GT 958 ACATGT BbsI 954 GAAGAC GAAGAC(2/6) 2704 GAAGAC BfaI 157 CTAG C^TAG 2648 CTAG BpuAI 954 GAAGAC GAAGAC(2/6) 2704 GAAGAC BstUI 9 CGCG CG^CG 117 CGCG BstXI 800 CCACATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 942 CCAGTTGGATGG CfoI 2448 GCGC GCG^C 2645 GCGC DraIII 1361 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 2412 CACCGAGTG DsaI 116 CCGCGG C^C[GA][CT]GG 654 CCATGG EaeI 617 TGGCCA [CT]^GGCC[GA] 1719 TGGCCG HaeII 2447 AGCGCC [GA]GCGC^[CT] 2644 AGCGCT HapII 26 CCGG C^CGG 100 CCGG HgaI 175 GACGC GACGC(5/10) 584 GCGTC HhaI 2448 GCGC GCG^C 2645 GCGC HinP1I 2448 GCGC G^CGC 2645 GCGC HpaII 26 CCGG C^CGG 100 CCGG MaeI 157 CTAG C^TAG 2648 CTAG MspI 26 CCGG C^CGG 100 CCGG MvnI 9 CGCG CG^CG 117 CGCG NdeI 2552 CATATG CA^TATG 2675 CATATG NspI 958 ACATGT [GA]CATG^[CT] 1684 ACATGC PpuMI 1114 AGGACCT [GA]G^G[AT]CC[CT] 2660 AGGTCCC PstI 915 CTGCAG CTGCA^G 2027 CTGCAG PvuII 711 CAGCTG CAG^CTG 1128 CAGCTG SmlI 757 CTTGAG C^T[CT][GA]AG 2758 CTTAAG SspI 310 AATATT AAT^ATT 2021 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AspHI AvaI AvaII BanII BbvI BcgI BcnI BmyI BpmI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI