(Circular) (Six-base) MAPSORT of: pFN21AE2478.seq Check: 6204 from: 1 to: 7618 pFN21AE2478 7618 bp With 182 enzymes: SgfI * July 26, 2013 12:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2510 2510 Size: 7618 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5428 278 Size: 53 83 186 936 3892 2468 Fragments arranged by size: 3892 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4961 1937 Size: 3024 4594 Acc65I G'GTAC_C Cuts at: 4939 4939 Size: 7618 AclI AA'CG_TT Cuts at: 1563 5745 6118 1563 Size: 4182 373 3063 Fragments arranged by size: 4182 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3432 4104 5681 7065 7333 1632 Size: 1800 672 1577 1384 268 1917 Fragments arranged by size: 1917 1800 1577 1384 672 268 AfeI AGC'GCT Cuts at: 4651 4651 Size: 7618 AflII C'TTAA_G Cuts at: 820 1017 4763 820 Size: 197 3746 3675 Fragments arranged by size: 3746 3675 197 AflIII A'CryG_T Cuts at: 1203 2858 2963 6517 7494 1203 Size: 1655 105 3554 977 1327 Fragments arranged by size: 3554 1655 1327 977 105 AgeI A'CCGG_T Cuts at: 6449 6449 Size: 7618 AhdI GACnn_n'nnGTC Cuts at: 6347 6347 Size: 7618 AleI CACnn'nnGTG Cuts at: 1166 2685 2810 3359 1166 Size: 1519 125 549 5425 Fragments arranged by size: 5425 1519 549 125 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7618 AlwNI CAG_nnn'CTG Cuts at: 5361 6933 5361 Size: 1572 6046 ApaLI G'TGCA_C Cuts at: 5675 6831 5675 Size: 1156 6462 ApoI r'AATT_y Cuts at: 1493 4253 4927 5153 5305 7414 1493 Size: 2760 674 226 152 2109 1697 Fragments arranged by size: 2760 2109 1697 674 226 152 AseI AT'TA_AT Cuts at: 160 1032 6172 160 Size: 872 5140 1606 Fragments arranged by size: 5140 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 AvaI C'yCGr_G Cuts at: 1870 1943 2030 3617 4845 4943 1870 Size: 73 87 1587 1228 98 4545 Fragments arranged by size: 4545 1587 1228 98 87 73 BamHI G'GATC_C Cuts at: 4948 5315 4948 Size: 367 7251 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2673 4939 6394 618 Size: 325 217 627 886 2266 1455 1842 Fragments arranged by size: 2266 1842 1455 886 627 325 217 BanII G_rGCy'C Cuts at: 1391 2286 3348 3721 4937 1391 Size: 895 1062 373 1216 4072 Fragments arranged by size: 4072 1216 1062 895 373 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2966 4716 928 Size: 971 1067 1750 3830 Fragments arranged by size: 3830 1750 1067 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3007 3041 3901 3935 4083 4117 4447 Size: 34 1070 34 860 34 148 34 330 Cuts at: 4447 4481 5809 5843 1903 Size: 34 1328 34 3678 Fragments arranged by size: 3678 1328 1070 860 330 148 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2893 5510 6720 2893 Size: 2617 1210 3791 Fragments arranged by size: 3791 2617 1210 BclI T'GATC_A Cuts at: 1548 3007 1548 Size: 1459 6159 BfrBI ATG'CAT Cuts at: 4739 4739 Size: 7618 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6229 136 Size: 107 122 71 631 605 4557 1525 Fragments arranged by size: 4557 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4479 7613 4479 Size: 3134 4484 BlpI GC'TnA_GC Cuts at: 3606 5035 3606 Size: 1429 6189 Bme1580I G_kGCm'C Cuts at: 1790 2155 4066 4073 5679 6835 1790 Size: 365 1911 7 1606 1156 2573 Fragments arranged by size: 2573 1911 1606 1156 365 7 BmgBI CAC'GTC Cuts at: 1318 3925 1318 Size: 2607 5011 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4226 4321 4380 Size: 416 53 83 399 24 2809 95 59 Cuts at: 4380 5349 6307 442 Size: 969 958 1753 Fragments arranged by size: 2809 1753 969 958 416 399 95 83 59 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7618 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2804 2930 3884 4585 6278 1239 Size: 1184 381 126 954 701 1693 2579 Fragments arranged by size: 2579 1693 1184 954 701 381 126 Bpu10I CC'TnA_GC Cuts at: 2528 3812 2528 Size: 1284 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2783 5743 6608 6906 2783 Size: 2960 865 298 3495 Fragments arranged by size: 3495 2960 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4900 6281 882 Size: 617 3401 1381 2219 Fragments arranged by size: 3401 2219 1381 617 BsaAI yAC'GTr Cuts at: 493 1204 3679 4428 4675 5409 493 Size: 711 2475 749 247 734 2702 Fragments arranged by size: 2702 2475 749 734 711 247 BsaBI GATnn'nnATC Cuts at: 7612 7612 Size: 7618 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2181 5425 5807 6442 Size: 53 83 186 936 648 3244 382 635 Cuts at: 6442 275 Size: 1451 Fragments arranged by size: 3244 1451 936 648 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6051 6449 6723 6870 7281 6051 Size: 398 274 147 411 6388 Fragments arranged by size: 6388 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2201 2231 2283 2313 1178 Size: 30 165 30 798 30 52 30 6483 Fragments arranged by size: 6483 798 165 52 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2767 3417 3483 4131 1186 Size: 1581 650 66 648 4673 Fragments arranged by size: 4673 1581 650 648 66 BseYI C'CCAG_C Cuts at: 4357 4408 6821 7317 4357 Size: 51 2413 496 4658 Fragments arranged by size: 4658 2413 496 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2623 2734 3244 3365 1163 Size: 573 887 111 510 121 5416 Fragments arranged by size: 5416 887 573 510 121 111 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5068 5829 6857 1540 Size: 275 186 3067 761 1028 2301 Fragments arranged by size: 3067 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2219 2286 3110 5679 5764 6835 2219 Size: 67 824 2569 85 1071 3002 Fragments arranged by size: 3002 2569 1071 824 85 67 BsmI GAATG_Cn' Cuts at: 2505 3287 3435 5129 5222 2505 Size: 782 148 1694 93 4901 Fragments arranged by size: 4901 1694 782 148 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3399 3399 Size: 7618 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2219 2286 3110 3348 3721 4066 Size: 399 365 64 67 824 238 373 345 Cuts at: 4066 4073 4937 5679 5764 6835 1391 Size: 7 864 742 85 1071 2174 Fragments arranged by size: 2174 1071 864 824 742 399 373 365 345 238 85 67 64 7 BspEI T'CCGG_A Cuts at: 7281 7281 Size: 7618 BspHI T'CATG_A Cuts at: 2365 5507 7237 2365 Size: 3142 1730 2746 Fragments arranged by size: 3142 2746 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2510 3241 4574 4973 844 Size: 1666 731 1333 399 3489 Fragments arranged by size: 3489 1666 1333 731 399 BsrBI CCG'CTC Cuts at: 1394 1823 2514 5505 1394 Size: 429 691 2991 3507 Fragments arranged by size: 3507 2991 691 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3001 6113 6287 65 Size: 1183 1753 3112 174 1396 Fragments arranged by size: 3112 1753 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6262 6449 1700 Size: 110 4452 187 2869 Fragments arranged by size: 4452 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7618 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7618 BssSI C'ACGA_G Cuts at: 5678 6690 5678 Size: 1012 6606 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7618 BstBI TT'CG_AA Cuts at: 6422 6422 Size: 7618 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7618 BstXI CCAn_nnnn'nTGG Cuts at: 2812 2954 2812 Size: 142 7476 BstYI r'GATC_y Cuts at: 1972 4479 4948 5315 5700 5717 7169 7255 7267 Size: 2507 469 367 385 17 1452 86 12 Cuts at: 7267 7613 1972 Size: 346 1977 Fragments arranged by size: 2507 1977 1452 469 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3741 3741 Size: 7618 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 2659 513 Size: 552 939 117 538 5472 Fragments arranged by size: 5472 939 552 538 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4573 487 Size: 683 56 3347 3532 Fragments arranged by size: 3532 3347 683 56 BtsI GCAGTG_nn' Cuts at: 2075 2258 2917 3382 5134 5928 5948 2075 Size: 183 659 465 1752 794 20 3745 Fragments arranged by size: 3745 1752 794 659 465 183 20 ClaI AT'CG_AT Cuts at: 1554 2265 1554 Size: 711 6907 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2906 2941 529 Size: 35 2342 35 5206 Fragments arranged by size: 5206 2342 35 35 DraI TTT'AAA Cuts at: 3064 4922 3064 Size: 1858 5760 DraIII CAC_nnn'GTG Cuts at: 3371 4422 3371 Size: 1051 6567 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6625 809 Size: 507 5309 1802 Fragments arranged by size: 5309 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2622 3724 5065 Size: 54 1425 50 275 18 792 1102 1341 Cuts at: 5065 5954 7583 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1341 1102 889 792 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5065 1537 Size: 275 3253 4090 Fragments arranged by size: 4090 3253 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 3451 3490 4540 5548 1353 Size: 1161 937 39 1050 1008 3423 Fragments arranged by size: 3423 1161 1050 1008 937 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6205 6579 6725 387 Size: 1064 138 4616 374 146 1280 Fragments arranged by size: 4616 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2284 2284 Size: 7618 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2804 2930 3432 3884 4104 4585 Size: 393 791 381 126 502 452 220 481 Cuts at: 4585 5681 6278 7065 7333 1239 Size: 1096 597 787 268 1524 Fragments arranged by size: 1524 1096 791 787 597 502 481 452 393 381 268 220 126 EcoO109I rG'GnC_Cy Cuts at: 2524 2575 3120 3462 4666 2524 Size: 51 545 342 1204 5476 Fragments arranged by size: 5476 1204 545 342 51 EcoRI G'AATT_C Cuts at: 4927 5305 4927 Size: 378 7240 FspI TGC'GCA Cuts at: 6124 6124 Size: 7618 HaeII r_GCGC'y Cuts at: 1678 4456 4653 6765 1678 Size: 2778 197 2112 2531 Fragments arranged by size: 2778 2531 2112 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2283 2315 2476 2508 2628 Size: 32 135 32 711 32 161 32 120 Cuts at: 2628 2660 2928 2960 3068 3092 3100 3124 4854 Size: 32 268 32 108 24 8 24 1730 Cuts at: 4854 4886 6259 6291 6333 6365 1373 Size: 32 1373 32 42 32 2626 Fragments arranged by size: 2626 1730 1373 711 268 161 135 120 108 42 32 32 32 32 32 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2437 2869 4016 4962 5208 669 Size: 988 281 499 432 1147 946 246 3079 Fragments arranged by size: 3079 1147 988 946 499 432 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7618 HpaI GTT'AAC Cuts at: 5208 5208 Size: 7618 KpnI G_GTAC'C Cuts at: 4943 4943 Size: 7618 MfeI C'AATT_G Cuts at: 5217 5217 Size: 7618 MluI A'CGCG_T Cuts at: 7494 7494 Size: 7618 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2914 2931 6732 6916 1305 Size: 1609 17 3801 184 2007 Fragments arranged by size: 3801 2007 1609 184 17 MscI TGG'CCA Cuts at: 10 64 1489 1832 2624 10 Size: 54 1425 343 792 5004 Fragments arranged by size: 5004 1425 792 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2430 2685 2745 2810 3359 5576 5935 Size: 648 1264 255 60 65 549 2217 359 Cuts at: 5935 6094 518 Size: 159 2042 Fragments arranged by size: 2217 2042 1264 648 549 359 255 159 65 60 MspA1I CmG'CkG Cuts at: 1606 2123 2718 3135 5034 5711 6859 7104 1606 Size: 517 595 417 1899 677 1148 245 2120 Fragments arranged by size: 2120 1899 1148 677 595 517 417 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2659 513 Size: 552 939 655 5472 Fragments arranged by size: 5472 939 655 552 NdeI CA'TA_TG Cuts at: 387 4558 4681 387 Size: 4171 123 3324 Fragments arranged by size: 4171 3324 123 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7618 NotI GC'GGCC_GC Cuts at: 5065 5065 Size: 7618 NsiI A_TGCA'T Cuts at: 4741 4741 Size: 7618 NspI r_CATG'y Cuts at: 1132 1229 2967 3693 4976 6521 1132 Size: 97 1738 726 1283 1545 2229 Fragments arranged by size: 2229 1738 1545 1283 726 97 PasI CC'CwG_GG Cuts at: 1348 3296 4066 1348 Size: 1948 770 4900 Fragments arranged by size: 4900 1948 770 PciI A'CATG_T Cuts at: 2963 6517 2963 Size: 3554 4064 PflMI CCAn_nnn'nTGG Cuts at: 1108 3097 1108 Size: 1989 5629 PfoI T'CCnGG_A Cuts at: 2781 2781 Size: 7618 PmeI GTTT'AAAC Cuts at: 4922 4922 Size: 7618 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5687 5719 7200 7232 5687 Size: 32 1481 32 6073 Fragments arranged by size: 6073 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3120 4666 3120 Size: 1546 6072 PshAI GACnn'nnGTC Cuts at: 1613 2912 1613 Size: 1299 6319 PsiI TTA'TAA Cuts at: 5188 5188 Size: 7618 PstI C_TGCA'G Cuts at: 830 2924 4036 4970 830 Size: 2094 1112 934 3478 Fragments arranged by size: 3478 2094 1112 934 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7618 PvuII CAG'CTG Cuts at: 2718 3135 2718 Size: 417 7201 SacI G_AGCT'C Cuts at: 2286 2286 Size: 7618 SacII CC_GC'GG Cuts at: 2124 2124 Size: 7618 SalI G'TCGA_C Cuts at: 1936 4960 1936 Size: 3024 4594 SapI GCTCTTCn'nnn_ Cuts at: 3490 3490 Size: 7618 SbfI CC_TGCA'GG Cuts at: 4970 4970 Size: 7618 ScaI AGT'ACT Cuts at: 5866 7578 5866 Size: 1712 5906 SexAI A'CCwGG_T Cuts at: 4018 4018 Size: 7618 SfcI C'TryA_G Cuts at: 826 1045 2554 2920 4032 4966 6101 6782 6973 Size: 219 1509 366 1112 934 1135 681 191 Cuts at: 6973 826 Size: 1471 Fragments arranged by size: 1509 1471 1135 1112 934 681 366 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7618 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7618 SmaI CCC'GGG Cuts at: 1872 2032 4945 1872 Size: 160 2913 4545 Fragments arranged by size: 4545 2913 160 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 4763 5722 6623 6885 820 Size: 197 926 819 2001 959 901 262 1553 Fragments arranged by size: 2001 1553 959 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 3679 5409 493 Size: 3186 1730 2702 Fragments arranged by size: 3186 2702 1730 SpeI A'CTAG_T Cuts at: 152 152 Size: 7618 SphI G_CATG'C Cuts at: 1132 4976 1132 Size: 3844 3774 SspI AAT'ATT Cuts at: 5 52 2317 4028 5542 5 Size: 47 2265 1711 1514 2081 Fragments arranged by size: 2265 2081 1711 1514 47 StuI AGG'CCT Cuts at: 1517 2160 2608 4122 1517 Size: 643 448 1514 5013 Fragments arranged by size: 5013 1514 643 448 StyI C'CwwG_G Cuts at: 513 1065 2004 2155 2236 2579 2659 5057 513 Size: 552 939 151 81 343 80 2398 3074 Fragments arranged by size: 3074 2398 939 552 343 151 81 80 SwaI ATTT'AAAT Cuts at: 3064 3064 Size: 7618 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2215 2661 3215 5645 5662 5815 6000 Size: 1255 635 446 554 2430 17 153 185 Cuts at: 6000 6445 325 Size: 445 1498 Fragments arranged by size: 2430 1498 1255 635 554 446 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 4827 5864 Size: 274 80 33 51 1443 753 2097 1037 Cuts at: 5864 7576 96 Size: 1712 138 Fragments arranged by size: 2097 1712 1443 1037 753 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7578 7610 7578 Size: 32 7586 Tth111I GACn'n_nGTC Cuts at: 1316 2777 1316 Size: 1461 6157 XbaI T'CTAG_A Cuts at: 4954 4954 Size: 7618 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2666 2812 2993 3293 4085 4253 2666 Size: 146 181 300 792 168 6031 Fragments arranged by size: 6031 792 300 181 168 146 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7618 XmaI C'CCGG_G Cuts at: 1870 2030 4943 1870 Size: 160 2913 4545 Fragments arranged by size: 4545 2913 160 XmnI GAAnn'nnTTC Cuts at: 4820 5747 4820 Size: 927 6691 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5426 276 Size: 53 83 186 936 3892 2468 Fragments arranged by size: 3892 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI