B3GALNT2 ORF clone pFN21AE2476

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Halotag ORF Clone pFN21AE2476

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Clone info.

Species

human

Clone Name

pFN21AE2476 Sequence (with vector) Restriction Map

Product ID

FHC12989

ORF Sequence

DNA sequence (1500 bp)
Protein sequence (500 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2476

Symbol

B3GALNT2

Alias : B3GalNAc-T2, MDDGA11

Description

beta-1,3-N-acetylgalactosaminyltransferase 2, transcript variant 1

Original Clone ID

oc01533

Note

We obtained this clone using the E.coli strain JM109.

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1500 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 500 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS1606.1 0 100.0 B3GALNT2 CCDS60453.1 8.3e-112 86.9 B3GALNT2 CCDS13.1 8.5e-15 28.3 B3GALT6 CCDS2227.1 6.2e-13 25.3 B3GALT1 CCDS1383.1 1.3e-12 28.7 B3GALT2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR002659 308 456 PF01762 Glycosyl transferase
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 3 NWLVLLCPCVLGAALHLWLR 22 PRIMARY 20 2 143 RVVSVSFRVLYPIVITSLGVFYD 165 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_689703 500 610194,615181 100.0 100.0 XP_006711812 586 610194,615181 99.8 98.6 XP_016855883 541 610194,615181 92.3 98.2 XP_016855884 485 610194,615181 91.3 85.6 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2476 Download

>KIEE2476  1500 bp
ATGCGAAACTGGCTGGTGCTGCTGTGCCCGTGTGTGCTCGGGGCCGCGCTGCACCTCTGG
CTGCGGCTGCGCTCCCCGCCGCCCGCCTGCGCCTCCGGGGCCGGCCCTGCAGATCAGTTG
GCCTTATTTCCTCAGTGGAAATCTACTCACTATGATGTGGTAGTTGGCGTGTTGTCAGCT
CGCAATAACCATGAACTTCGAAACGTGATAAGAAGCACCTGGATGAGACATTTGCTACAG
CATCCCACATTAAGTCAACGTGTGCTTGTGAAGTTCATAATAGGTGCTCATGGCTGTGAA
GTGCCTGTGGAAGACAGGGAAGATCCTTATTCCTGTAAACTACTCAACATCACAAATCCA
GTTTTGAATCAGGAAATTGAAGCGTTCAGTCTGTCCGAAGACACTTCATCGGGGCTGCCT
GAGGATCGAGTTGTCAGCGTGAGTTTCCGAGTTCTCTACCCCATCGTTATTACCAGTCTT
GGAGTGTTCTACGATGCCAATGATGTGGGTTTCCAGAGGAACATCACTGTCAAACTTTAT
CAGGCAGAACAAGAGGAGGCCCTCTTCATTGCTCGCTTCAGTCCTCCAAGCTGTGGTGTG
CAGGTGAACAAGCTGTGGTACAAGCCCGTGGAACAATTCATCTTACCAGAGAGCTTTGAA
GGTACAATCGTGTGGGAGAGCCAAGACCTCCACGGCCTTGTGTCAAGAAATCTCCACAAA
GTGACAGTGAATGATGGAGGGGGAGTTCTCAGAGTCATTACAGCTGGGGAGGGTGCATTG
CCTCATGAATTCTTGGAAGGTGTGGAGGGAGTTGCAGGTGGTTTTATATATACTATTCAG
GAAGGTGATGCTCTCTTACACAACCTTCATTCTCGCCCTCAAAGACTTATTGATCATATA
AGGAATCTCCATGAGGAAGATGCCTTACTGAAGGAGGAAAGCAGCATCTATGATGATATT
GTTTTTGTGGATGTTGTCGACACTTATCGTAATGTTCCTGCAAAATTATTGAACTTCTAT
AGATGGACTGTGGAAACAACGAGCTTCAATTTGTTGCTGAAGACAGATGATGACTGTTAC
ATAGACCTCGAAGCTGTATTTAATAGGATTGTCCAAAAGAATCTGGATGGGCCTAATTTT
TGGTGGGGAAATTTCAGACTGAATTGGGCAGTTGACCGAACCGGAAAGTGGCAGGAGTTG
GAGTACCCGAGCCCCGCTTACCCTGCCTTTGCATGTGGGTCAGGATATGTGATCTCCAAG
GACATCGTCAAGTGGCTGGCAAGCAACTCGGGGAGGTTAAAGACCTATCAGGGTGAAGAT
GTAAGCATGGGCATCTGGATGGCTGCCATAGGACCTAAAAGATACCAGGACAGTCTGTGG
CTGTGTGAGAAGACCTGTGAGACAGGAATGCTGTCTTCTCCTCAGTATTCTCCGTGGGAA
CTGACGGAACTGTGGAAACTGAAGGAACGGTGCGGTGATCCTTGTCGATGTCAAGCAAGA

ORF Protein

Cloned ORF protein sequence for pFN21AE2476 Download

>KIEE2476  500 aa
MRNWLVLLCPCVLGAALHLWLRLRSPPPACASGAGPADQLALFPQWKSTHYDVVVGVLSA
RNNHELRNVIRSTWMRHLLQHPTLSQRVLVKFIIGAHGCEVPVEDREDPYSCKLLNITNP
VLNQEIEAFSLSEDTSSGLPEDRVVSVSFRVLYPIVITSLGVFYDANDVGFQRNITVKLY
QAEQEEALFIARFSPPSCGVQVNKLWYKPVEQFILPESFEGTIVWESQDLHGLVSRNLHK
VTVNDGGGVLRVITAGEGALPHEFLEGVEGVAGGFIYTIQEGDALLHNLHSRPQRLIDHI
RNLHEEDALLKEESSIYDDIVFVDVVDTYRNVPAKLLNFYRWTVETTSFNLLLKTDDDCY
IDLEAVFNRIVQKNLDGPNFWWGNFRLNWAVDRTGKWQELEYPSPAYPAFACGSGYVISK
DIVKWLASNSGRLKTYQGEDVSMGIWMAAIGPKRYQDSLWLCEKTCETGMLSSPQYSPWE
LTELWKLKERCGDPCRCQAR

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2476 Download

>pFN21AE2476  6205 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGCGAAACTGGCTGGTGCTGCTGTGCCCGTGTGT
GCTCGGGGCCGCGCTGCACCTCTGGCTGCGGCTGCGCTCCCCGCCGCCCGCCTGCGCCTC
CGGGGCCGGCCCTGCAGATCAGTTGGCCTTATTTCCTCAGTGGAAATCTACTCACTATGA
TGTGGTAGTTGGCGTGTTGTCAGCTCGCAATAACCATGAACTTCGAAACGTGATAAGAAG
CACCTGGATGAGACATTTGCTACAGCATCCCACATTAAGTCAACGTGTGCTTGTGAAGTT
CATAATAGGTGCTCATGGCTGTGAAGTGCCTGTGGAAGACAGGGAAGATCCTTATTCCTG
TAAACTACTCAACATCACAAATCCAGTTTTGAATCAGGAAATTGAAGCGTTCAGTCTGTC
CGAAGACACTTCATCGGGGCTGCCTGAGGATCGAGTTGTCAGCGTGAGTTTCCGAGTTCT
CTACCCCATCGTTATTACCAGTCTTGGAGTGTTCTACGATGCCAATGATGTGGGTTTCCA
GAGGAACATCACTGTCAAACTTTATCAGGCAGAACAAGAGGAGGCCCTCTTCATTGCTCG
CTTCAGTCCTCCAAGCTGTGGTGTGCAGGTGAACAAGCTGTGGTACAAGCCCGTGGAACA
ATTCATCTTACCAGAGAGCTTTGAAGGTACAATCGTGTGGGAGAGCCAAGACCTCCACGG
CCTTGTGTCAAGAAATCTCCACAAAGTGACAGTGAATGATGGAGGGGGAGTTCTCAGAGT
CATTACAGCTGGGGAGGGTGCATTGCCTCATGAATTCTTGGAAGGTGTGGAGGGAGTTGC
AGGTGGTTTTATATATACTATTCAGGAAGGTGATGCTCTCTTACACAACCTTCATTCTCG
CCCTCAAAGACTTATTGATCATATAAGGAATCTCCATGAGGAAGATGCCTTACTGAAGGA
GGAAAGCAGCATCTATGATGATATTGTTTTTGTGGATGTTGTCGACACTTATCGTAATGT
TCCTGCAAAATTATTGAACTTCTATAGATGGACTGTGGAAACAACGAGCTTCAATTTGTT
GCTGAAGACAGATGATGACTGTTACATAGACCTCGAAGCTGTATTTAATAGGATTGTCCA
AAAGAATCTGGATGGGCCTAATTTTTGGTGGGGAAATTTCAGACTGAATTGGGCAGTTGA
CCGAACCGGAAAGTGGCAGGAGTTGGAGTACCCGAGCCCCGCTTACCCTGCCTTTGCATG
TGGGTCAGGATATGTGATCTCCAAGGACATCGTCAAGTGGCTGGCAAGCAACTCGGGGAG
GTTAAAGACCTATCAGGGTGAAGATGTAAGCATGGGCATCTGGATGGCTGCCATAGGACC
TAAAAGATACCAGGACAGTCTGTGGCTGTGTGAGAAGACCTGTGAGACAGGAATGCTGTC
TTCTCCTCAGTATTCTCCGTGGGAACTGACGGAACTGTGGAAACTGAAGGAACGGTGCGG
TGATCCTTGTCGATGTCAAGCAAGAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCC
TCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAG
CTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTT
CGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGA
AAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGC
TGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAG
ATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAA
TGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATC
CGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGT
GGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCA
AATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGG
AAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC
CTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTG
GGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTT
CGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTA
TTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAAT
GACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGA
GAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACA
ACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACT
CGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACC
ACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACT
CTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTT
CTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGT
GGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTT
ATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATA
GGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAG
GAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAG
TGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTA
AAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTC
TGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAA
ATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATG
CGTCCGGCGTAGAGGATCGAGATCT