(Circular) (Six-base) MAPSORT of: pFN21AE2476.seq Check: 7828 from: 1 to: 6205 pFN21AE2476 6205 bp With 182 enzymes: SgfI * June 18, 2013 12:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2596 2810 2596 Size: 214 5991 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4015 278 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2982 3548 1937 Size: 1045 566 4594 Fragments arranged by size: 4594 1045 566 Acc65I G'GTAC_C Cuts at: 3526 3526 Size: 6205 AclI AA'CG_TT Cuts at: 1563 4332 4705 1563 Size: 2769 373 3063 Fragments arranged by size: 3063 2769 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2566 2954 3083 3485 4268 5652 5920 1632 Size: 934 388 129 402 783 1384 268 1917 Fragments arranged by size: 1917 1384 934 783 402 388 268 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6008 AflIII A'CryG_T Cuts at: 1203 2263 5104 6081 1203 Size: 1060 2841 977 1327 Fragments arranged by size: 2841 1327 1060 977 AgeI A'CCGG_T Cuts at: 5036 5036 Size: 6205 AhdI GACnn_n'nnGTC Cuts at: 4934 4934 Size: 6205 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6205 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2733 2765 2733 Size: 32 6173 AlwNI CAG_nnn'CTG Cuts at: 3948 5520 3948 Size: 1572 4633 ApaLI G'TGCA_C Cuts at: 4262 5418 4262 Size: 1156 5049 ApoI r'AATT_y Cuts at: 1493 2792 3154 3514 3740 3892 6001 1493 Size: 1299 362 360 226 152 2109 1697 Fragments arranged by size: 2109 1697 1299 362 360 226 152 AseI AT'TA_AT Cuts at: 160 1032 4759 160 Size: 872 3727 1606 Fragments arranged by size: 3727 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 AvaI C'yCGr_G Cuts at: 1870 1943 2042 3211 3292 3530 1870 Size: 73 99 1169 81 238 4545 Fragments arranged by size: 4545 1169 238 99 81 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2658 2691 2658 Size: 33 6172 BamHI G'GATC_C Cuts at: 3535 3902 3535 Size: 367 5838 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3526 4981 618 Size: 325 217 627 1739 1455 1842 Fragments arranged by size: 1842 1739 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3218 3524 1391 Size: 1827 306 4072 Fragments arranged by size: 4072 1827 306 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 2409 3071 3401 3411 928 Size: 971 423 87 662 330 10 3722 Fragments arranged by size: 3722 971 662 423 330 87 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3273 3307 4396 4430 1903 Size: 34 50 34 1252 34 1089 34 3678 Fragments arranged by size: 3678 1252 1089 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4097 5307 4097 Size: 1210 4995 BclI T'GATC_A Cuts at: 1548 2896 1548 Size: 1348 4857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 4816 136 Size: 107 122 71 631 605 430 2714 1525 Fragments arranged by size: 2714 1525 631 605 430 122 107 71 BglII A'GATC_T Cuts at: 6200 6200 Size: 6205 BlpI GC'TnA_GC Cuts at: 3622 3622 Size: 6205 Bme1580I G_kGCm'C Cuts at: 1790 2033 4266 5422 1790 Size: 243 2233 1156 2573 Fragments arranged by size: 2573 2233 1156 243 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6205 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3936 4894 442 Size: 416 53 83 399 24 2519 958 1753 Fragments arranged by size: 2519 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6205 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4865 1239 Size: 3626 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4330 5195 5493 4330 Size: 865 298 5042 Fragments arranged by size: 5042 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4868 882 Size: 617 3369 2219 Fragments arranged by size: 3369 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3996 493 Size: 711 2792 2702 Fragments arranged by size: 2792 2702 711 BsaBI GATnn'nnATC Cuts at: 6199 6199 Size: 6205 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4012 4394 5029 275 Size: 53 83 186 936 2479 382 635 1451 Fragments arranged by size: 2479 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3185 4638 5036 5310 5457 5868 3185 Size: 1453 398 274 147 411 3522 Fragments arranged by size: 3522 1453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2733 2763 3191 3221 1178 Size: 30 165 30 1330 30 428 30 4162 Fragments arranged by size: 4162 1330 428 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2573 3414 1186 Size: 1387 841 3977 Fragments arranged by size: 3977 1387 841 BseYI C'CCAG_C Cuts at: 2768 5408 5904 2768 Size: 2640 496 3069 Fragments arranged by size: 3069 2640 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 2624 1163 Size: 573 303 585 4744 Fragments arranged by size: 4744 585 573 303 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3655 4416 5444 1540 Size: 275 186 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 2293 4266 4351 5422 2043 Size: 250 1973 85 1071 2826 Fragments arranged by size: 2826 1973 1071 250 85 BsmI GAATG_Cn' Cuts at: 3417 3716 3809 3417 Size: 299 93 5813 Fragments arranged by size: 5813 299 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2043 2293 3218 3524 4266 4351 Size: 399 243 10 250 925 306 742 85 Cuts at: 4351 5422 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 925 742 399 306 250 243 85 10 BspEI T'CCGG_A Cuts at: 5868 5868 Size: 6205 BspHI T'CATG_A Cuts at: 2788 4094 5824 2788 Size: 1306 1730 3169 Fragments arranged by size: 3169 1730 1306 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2596 2810 3560 844 Size: 1752 214 750 3489 Fragments arranged by size: 3489 1752 750 214 BsrBI CCG'CTC Cuts at: 1394 1823 4092 1394 Size: 429 2269 3507 Fragments arranged by size: 3507 2269 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2571 2780 4700 4874 65 Size: 1183 1323 209 1920 174 1396 Fragments arranged by size: 1920 1396 1323 1183 209 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 4849 5036 1700 Size: 110 295 2744 187 2869 Fragments arranged by size: 2869 2744 295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6205 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6205 BssSI C'ACGA_G Cuts at: 4265 5277 4265 Size: 1012 5193 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6205 BstBI TT'CG_AA Cuts at: 2203 5009 2203 Size: 2806 3399 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6205 BstXI CCAn_nnnn'nTGG Cuts at: 2509 2509 Size: 6205 BstYI r'GATC_y Cuts at: 1972 2326 3535 3902 4287 4304 5756 5842 5854 Size: 354 1209 367 385 17 1452 86 12 Cuts at: 5854 6200 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1209 385 367 354 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2424 2424 Size: 6205 BtgI C'CryG_G Cuts at: 513 1065 2631 2695 3437 513 Size: 552 1566 64 742 3281 Fragments arranged by size: 3281 1566 742 552 64 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5466 Fragments arranged by size: 5466 683 56 BtsI GCAGTG_nn' Cuts at: 3721 4515 4535 3721 Size: 794 20 5391 Fragments arranged by size: 5391 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6205 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6170 DraI TTT'AAA Cuts at: 3509 3509 Size: 6205 DraIII CAC_nnn'GTG Cuts at: 2725 2725 Size: 6205 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5212 809 Size: 507 3896 1802 Fragments arranged by size: 3896 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3652 4541 6170 Size: 54 1425 50 275 18 1822 889 1629 Cuts at: 6170 8 Size: 43 Fragments arranged by size: 1822 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3652 1537 Size: 275 1840 4090 Fragments arranged by size: 4090 1840 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2573 4135 1353 Size: 1220 1562 3423 Fragments arranged by size: 3423 1562 1220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4792 5166 5312 387 Size: 1064 138 3203 374 146 1280 Fragments arranged by size: 3203 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2566 2954 3083 3485 4268 4865 5652 Size: 393 934 388 129 402 783 597 787 Cuts at: 5652 5920 1239 Size: 268 1524 Fragments arranged by size: 1524 934 787 783 597 402 393 388 268 129 EcoO109I rG'GnC_Cy Cuts at: 2563 3356 2563 Size: 793 5412 EcoRI G'AATT_C Cuts at: 2792 3514 3892 2792 Size: 722 378 5105 Fragments arranged by size: 5105 722 378 FseI GG_CCGG'CC Cuts at: 2109 2109 Size: 6205 FspI TGC'GCA Cuts at: 4711 4711 Size: 6205 HaeII r_GCGC'y Cuts at: 1678 5352 1678 Size: 3674 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2396 2428 3240 3272 4846 Size: 32 135 32 824 32 812 32 1574 Cuts at: 4846 4878 4920 4952 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1574 824 812 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2261 2983 3178 3549 3795 669 Size: 988 281 323 722 195 371 246 3079 Fragments arranged by size: 3079 988 722 371 323 281 246 195 HindIII A'AGCT_T Cuts at: 748 748 Size: 6205 HpaI GTT'AAC Cuts at: 3795 3795 Size: 6205 KpnI G_GTAC'C Cuts at: 3530 3530 Size: 6205 MfeI C'AATT_G Cuts at: 3804 3804 Size: 6205 MluI A'CGCG_T Cuts at: 6081 6081 Size: 6205 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3183 5319 5503 1305 Size: 1878 2136 184 2007 Fragments arranged by size: 2136 2007 1878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4383 Fragments arranged by size: 4383 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2225 2298 3341 4163 4522 4681 Size: 648 991 68 73 1043 822 359 159 Cuts at: 4681 518 Size: 2042 Fragments arranged by size: 2042 1043 991 822 648 359 159 73 68 MspA1I CmG'CkG Cuts at: 1606 2768 3621 4298 5446 5691 1606 Size: 1162 853 677 1148 245 2120 Fragments arranged by size: 2120 1162 1148 853 677 245 NaeI GCC'GGC Cuts at: 2107 2107 Size: 6205 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5653 NdeI CA'TA_TG Cuts at: 387 387 Size: 6205 NgoMIV G'CCGG_C Cuts at: 2105 2105 Size: 6205 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6205 NotI GC'GGCC_GC Cuts at: 3652 3652 Size: 6205 NspI r_CATG'y Cuts at: 1132 1229 3240 3563 5108 1132 Size: 97 2011 323 1545 2229 Fragments arranged by size: 2229 2011 1545 323 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6205 PciI A'CATG_T Cuts at: 5104 5104 Size: 6205 PflMI CCAn_nnn'nTGG Cuts at: 1108 2597 1108 Size: 1489 4716 PmeI GTTT'AAAC Cuts at: 3509 3509 Size: 6205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2733 2765 4274 4306 5787 5819 2733 Size: 32 1509 32 1481 32 3119 Fragments arranged by size: 3119 1509 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3356 3356 Size: 6205 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6205 PsiI TTA'TAA Cuts at: 3775 3775 Size: 6205 PstI C_TGCA'G Cuts at: 830 2116 3557 830 Size: 1286 1441 3478 Fragments arranged by size: 3478 1441 1286 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6205 PvuII CAG'CTG Cuts at: 2768 2768 Size: 6205 SalI G'TCGA_C Cuts at: 1936 2981 3547 1936 Size: 1045 566 4594 Fragments arranged by size: 4594 1045 566 SbfI CC_TGCA'GG Cuts at: 3557 3557 Size: 6205 ScaI AGT'ACT Cuts at: 4453 6165 4453 Size: 1712 4493 SfcI C'TryA_G Cuts at: 826 1045 2112 2240 3022 3553 4688 5369 5560 Size: 219 1067 128 782 531 1135 681 191 Cuts at: 5560 826 Size: 1471 Fragments arranged by size: 1471 1135 1067 782 681 531 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6205 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6205 SmaI CCC'GGG Cuts at: 1872 3532 1872 Size: 1660 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4309 5210 5472 820 Size: 197 926 2366 901 262 1553 Fragments arranged by size: 2366 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3996 493 Size: 3503 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6205 SphI G_CATG'C Cuts at: 1132 3563 1132 Size: 2431 3774 SspI AAT'ATT Cuts at: 5 52 4129 5 Size: 47 4077 2081 Fragments arranged by size: 4077 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6205 StyI C'CwwG_G Cuts at: 513 1065 3261 3644 513 Size: 552 2196 383 3074 Fragments arranged by size: 3074 2196 552 383 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3195 4232 4249 4402 4587 5032 325 Size: 1255 1615 1037 17 153 185 445 1498 Fragments arranged by size: 1615 1498 1255 1037 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4451 6163 96 Size: 274 80 33 51 1443 2474 1712 138 Fragments arranged by size: 2474 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3365 3397 6165 6197 3365 Size: 32 2768 32 3373 Fragments arranged by size: 3373 2768 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3269 1316 Size: 1953 4252 XbaI T'CTAG_A Cuts at: 3541 3541 Size: 6205 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6205 XmaI C'CCGG_G Cuts at: 1870 3530 1870 Size: 1660 4545 XmnI GAAnn'nnTTC Cuts at: 4334 4334 Size: 6205 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4013 276 Size: 53 83 186 936 2479 2468 Fragments arranged by size: 2479 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI