################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MunI NarI NcoI NdeI NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 976 GTCGAC GT^[AC][GT]AC AccII 45 CGCG CG^CG AflIII 258 ACGTGT A^C[GA][CT]GT AocI 418 CCTGAGG CC^T[ACGT]AGG AspI 1261 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1351 GGACC G^G[AT]CC BanII 1209 GAGCCC G[GA]GC[CT]^C BcgI 1279 GCAAGCAACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 891 TGATCA T^GATCA BcnI 95 CCGGG CC^[GC]GG BglI 91 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 1180 ACCGGA [AT]^CCGG[AT] BsmI 1406 GAATGC GAATGC(1/-1) BspHI 783 TCATGA T^CATGA BsrFI 100 GCCGGC [GA]^CCGG[CT] BstBI 197 TTCGAA TT^CGAA BstUI 45 CGCG CG^CG BstXI 497 CCAATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 321 AGATCC [GA]^GATC[CT] Bsu36I 418 CCTGAGG CC^T[ACGT]AGG Cfr10I 100 GCCGGC [GA]^CCGG[CT] DraIII 715 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 562 CTCTTC CTCTTC(1/4) Eco81I 418 CCTGAGG CC^T[ACGT]AGG EcoRI 787 GAATTC G^AATTC EcoT14I 1256 CCAAGG C^C[AT][AT]GG FbaI 891 TGATCA T^GATCA FseI 99 GGCCGGCC GGCCGG^CC Ksp632I 562 CTCTTC CTCTTC(1/4) MflI 321 AGATCC [GA]^GATC[CT] MspA1I 761 CAGCTG C[AC]G^C[GT]G MvnI 45 CGCG CG^CG NaeI 100 GCCGGC GCC^GGC NciI 95 CCGGG CC^[GC]GG NgoMIV 100 GCCGGC G^CCGGC NspI 1231 GCATGT [GA]CATG^[CT] NspV 197 TTCGAA TT^CGAA PflFI 1261 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 586 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 752 GAGTC GAGTC(4/5) PpuMI 1350 AGGACCT [GA]G^G[AT]CC[CT] PstI 107 CTGCAG CTGCA^G PvuII 761 CAGCTG CAG^CTG RcaI 783 TCATGA T^CATGA SalI 976 GTCGAC G^TCGAC SfuI 197 TTCGAA TT^CGAA StyI 1256 CCAAGG C^C[AT][AT]GG Tsp45I 721 GTGAC ^GT[GC]AC Tth111I 1261 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 586 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 321 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 787 GAATTC [GA]^AATT[CT] 1149 AAATTT ApoI 787 GAATTC [GA]^AATT[CT] 1149 AAATTT AspHI 34 GTGCTC G[AT]GC[AT]^C 284 GTGCTC BseRI 1418 CTCCTC GAGGAG(10/8) 553 GAGGAG BsgI 49 CTGCAC GTGCAG(16/14) 598 GTGCAG BsiHKAI 34 GTGCTC G[AT]GC[AT]^C 284 GTGCTC BsiYI 586 CCAAGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1181 CCGGAAAGTGG BslI 586 CCAAGCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1181 CCGGAAAGTGG BsmAI 225 GAGAC GTCTC(1/5) 1399 GAGAC BspMI 600 GCAGGT ACCTGC(4/8) 814 GCAGGT BsrDI 567 CATTGC GCAATG(2/0) 776 CATTGC BstNI 218 CCTGG CC^[AT]GG 1365 CCAGG DraII 557 AGGCCCT [GA]G^G[ACGT]CC[CT] 1350 AGGACCT EcoO109I 557 AGGCCCT [GA]G^G[ACGT]CC[CT] 1350 AGGACCT EcoRII 218 CCTGG ^CC[AT]GG 1365 CCAGG MaeII 203 ACGT A^CGT 258 ACGT MaeIII 721 GTGAC ^GT[ACGT]AC 1076 GTTAC MvaI 218 CCTGG CC^[AT]GG 1365 CCAGG NlaIV 40 GGGGCC GG[ACGT]^[ACGT]CC 97 GGGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI BbsI BbvI BmyI BpuAI BsaJI BsoBI Bsp1286I BsrI BssKI BstF5I Cac8I CfoI Cfr13I DdeI DpnII DsaI FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI MboI MboII MnlI MseI MslI MspI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I