METTL4 ORF clone pFN21AE2225

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Halotag ORF Clone pFN21AE2225

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Clone info.

Species

human

Clone Name

pFN21AE2225 Sequence (with vector) Restriction Map

Product ID

FHC11774

ORF Sequence

DNA sequence (1416 bp)
Protein sequence (472 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2225

Symbol

METTL4

Alias : HsT661

Description

methyltransferase like 4, transcript variant 1

Original Clone ID

cs03325

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1416 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 472 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS11826.1 2.4e-205 99.8 METTL4 CCDS77144.1 2.3e-169 99.7 METTL4 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007757 281 455 PF05063 MT-A70 ProfileScan IPR007757 241 472 PS51143 MT-A70 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2225 Download

>KIEE2225  1416 bp
ATGTCTGTGGTACACCAGTTGTCAGCTGGGTGGTTACTGGATCATCTTTCTTTTATCAAC
AAGATAAACTATCAACTTCACCAGCATCATGAACCTTGTTGCCGTAAAAAGGAGTTCACT
ACTTCTGTTCACTTTGAGTCTCTTCAAATGGATTCTGTGTCCTCCTCTGGAGTCTGTGCT
GCATTTATTGCTTCTGACTCTTCCACTAAGCCAGAGAATGATGATGGAGGAAATTATGAA
ATGTTCACACGAAAATTTGTTTTTCGACCTGAACTGTTTGATGTCACCAAACCTTATATA
ACTCCAGCTGTTCATAAAGAATGCCAGCAAAGTAATGAAAAGGAAGATCTGATGAATGGT
GTTAAAAAAGAAATCTCCATTTCTATTATTGGGAAGAAGCGTAAAAGATGTGTTGTTTTC
AATCAAGGTGAATTGGATGCTATGGAATACCATACAAAGATCAGGGAGCTGATTTTGGAT
GGATCTTTACAGTTGATCCAGGAAGGTCTCAAAAGTGGTTTTCTTTATCCACTTTTTGAA
AAACAGGACAAGGGTAGTAAGCCCATTACTTTACCACTTGACGCCTGCAGTTTGTCAGAA
TTATGTGAAATGGCAAAGCATTTGCCTTCTCTGAATGAAATGGAACATCAGACATTACAA
TTGGTGGAAGAGGATACATCTGTTACAGAACAGGATTTATTTTTGCGAGTTGTTGAAAAC
AACTCTAGCTTTACAAAAGTGATTACTTTAATGGGACAGAAATACCTGCTACCACCGAAA
AGCAGTTTTCTTTTATCTGACATTTCTTGTATGCAACCACTTCTAAACTATAGGAAAACA
TTTGATGTAATTGTGATAGATCCACCATGGCAGAACAAATCAGTTAAAAGAAGTAATAGG
TACAGTTATTTGTCACCCCTGCAAATAAAGCAAATACCTATCCCTAAATTGGCTGCTCCA
AACTGTCTTCTTGTTACTTGGGTGACCAATAGACAGAAGCACCTACGTTTTATAAAGGAA
GAACTTTATCCCTCTTGGTCTGTGGAGGTAGTTGCTGAGTGGCACTGGGTAAAAATAACC
AATTCAGGAGAATTTGTGTTCCCATTAGATTCTCCACACAAAAAGCCCTACGAAGGTCTT
ATACTGGGGAGGGTTCAAGAAAAAACTGCTCTACCATTGAGGAATGCAGATGTAAACGTG
CTCCCCATTCCAGACCACAAATTAATTGTCAGCGTGCCCTGTACTCTTCACTCACATAAG
CCACCGCTTGCTGAGGTTTTAAAAGACTACATCAAGCCAGATGGGGAATATTTGGAGTTG
TTTGCTCGAAATTTACAGCCAGGTTGGACTAGTTGGGGCAATGAAGTTCTCAAATTTCAG
CATGTGGATTATTTTATTGCTGTGGAGTCTGGAAGC

ORF Protein

Cloned ORF protein sequence for pFN21AE2225 Download

>KIEE2225  472 aa
MSVVHQLSAGWLLDHLSFINKINYQLHQHHEPCCRKKEFTTSVHFESLQMDSVSSSGVCA
AFIASDSSTKPENDDGGNYEMFTRKFVFRPELFDVTKPYITPAVHKECQQSNEKEDLMNG
VKKEISISIIGKKRKRCVVFNQGELDAMEYHTKIRELILDGSLQLIQEGLKSGFLYPLFE
KQDKGSKPITLPLDACSLSELCEMAKHLPSLNEMEHQTLQLVEEDTSVTEQDLFLRVVEN
NSSFTKVITLMGQKYLLPPKSSFLLSDISCMQPLLNYRKTFDVIVIDPPWQNKSVKRSNR
YSYLSPLQIKQIPIPKLAAPNCLLVTWVTNRQKHLRFIKEELYPSWSVEVVAEWHWVKIT
NSGEFVFPLDSPHKKPYEGLILGRVQEKTALPLRNADVNVLPIPDHKLIVSVPCTLHSHK
PPLAEVLKDYIKPDGEYLELFARNLQPGWTSWGNEVLKFQHVDYFIAVESGS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2225 Download

>pFN21AE2225  6121 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCTGTGGTACACCAGTTGTCAGCTGGGTGGTT
ACTGGATCATCTTTCTTTTATCAACAAGATAAACTATCAACTTCACCAGCATCATGAACC
TTGTTGCCGTAAAAAGGAGTTCACTACTTCTGTTCACTTTGAGTCTCTTCAAATGGATTC
TGTGTCCTCCTCTGGAGTCTGTGCTGCATTTATTGCTTCTGACTCTTCCACTAAGCCAGA
GAATGATGATGGAGGAAATTATGAAATGTTCACACGAAAATTTGTTTTTCGACCTGAACT
GTTTGATGTCACCAAACCTTATATAACTCCAGCTGTTCATAAAGAATGCCAGCAAAGTAA
TGAAAAGGAAGATCTGATGAATGGTGTTAAAAAAGAAATCTCCATTTCTATTATTGGGAA
GAAGCGTAAAAGATGTGTTGTTTTCAATCAAGGTGAATTGGATGCTATGGAATACCATAC
AAAGATCAGGGAGCTGATTTTGGATGGATCTTTACAGTTGATCCAGGAAGGTCTCAAAAG
TGGTTTTCTTTATCCACTTTTTGAAAAACAGGACAAGGGTAGTAAGCCCATTACTTTACC
ACTTGACGCCTGCAGTTTGTCAGAATTATGTGAAATGGCAAAGCATTTGCCTTCTCTGAA
TGAAATGGAACATCAGACATTACAATTGGTGGAAGAGGATACATCTGTTACAGAACAGGA
TTTATTTTTGCGAGTTGTTGAAAACAACTCTAGCTTTACAAAAGTGATTACTTTAATGGG
ACAGAAATACCTGCTACCACCGAAAAGCAGTTTTCTTTTATCTGACATTTCTTGTATGCA
ACCACTTCTAAACTATAGGAAAACATTTGATGTAATTGTGATAGATCCACCATGGCAGAA
CAAATCAGTTAAAAGAAGTAATAGGTACAGTTATTTGTCACCCCTGCAAATAAAGCAAAT
ACCTATCCCTAAATTGGCTGCTCCAAACTGTCTTCTTGTTACTTGGGTGACCAATAGACA
GAAGCACCTACGTTTTATAAAGGAAGAACTTTATCCCTCTTGGTCTGTGGAGGTAGTTGC
TGAGTGGCACTGGGTAAAAATAACCAATTCAGGAGAATTTGTGTTCCCATTAGATTCTCC
ACACAAAAAGCCCTACGAAGGTCTTATACTGGGGAGGGTTCAAGAAAAAACTGCTCTACC
ATTGAGGAATGCAGATGTAAACGTGCTCCCCATTCCAGACCACAAATTAATTGTCAGCGT
GCCCTGTACTCTTCACTCACATAAGCCACCGCTTGCTGAGGTTTTAAAAGACTACATCAA
GCCAGATGGGGAATATTTGGAGTTGTTTGCTCGAAATTTACAGCCAGGTTGGACTAGTTG
GGGCAATGAAGTTCTCAAATTTCAGCATGTGGATTATTTTATTGCTGTGGAGTCTGGAAG
CGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T