(Circular) (Six-base) MAPSORT of: pFN21AE2225.seq Check: 8623 from: 1 to: 6121 pFN21AE2225 6121 bp With 182 enzymes: SgfI * July 6, 2012 17:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3464 1937 Size: 1527 4594 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4184 5568 5836 1632 Size: 2552 1384 268 1917 Fragments arranged by size: 2552 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 3864 5436 3864 Size: 1572 4549 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2258 3095 3334 3377 3430 3656 3808 5917 Size: 765 837 239 43 53 226 152 2109 Cuts at: 5917 1493 Size: 1697 Fragments arranged by size: 2109 1697 837 765 239 226 152 53 43 AseI AT'TA_AT Cuts at: 160 1032 3227 4675 160 Size: 872 2195 1448 1606 Fragments arranged by size: 2195 1606 1448 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2006 2039 2670 2703 2006 Size: 33 631 33 5424 Fragments arranged by size: 5424 631 33 33 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3442 4897 618 Size: 325 217 627 1655 1455 1842 Fragments arranged by size: 1842 1655 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3440 1391 Size: 2049 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2963 928 Size: 971 1064 4086 Fragments arranged by size: 4086 1064 971 BbvCI CC'TCA_GC Cuts at: 3276 3276 Size: 6121 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2671 4013 5223 2671 Size: 1342 1210 3569 Fragments arranged by size: 3569 1342 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2350 6116 2350 Size: 3766 2355 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 3243 4182 5338 1790 Size: 1453 939 1156 2573 Fragments arranged by size: 2573 1453 1156 939 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3079 3158 3852 Size: 416 53 83 399 24 1662 79 694 Cuts at: 3852 4810 442 Size: 958 1753 Fragments arranged by size: 1753 1662 958 694 416 399 83 79 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2193 2292 4781 1239 Size: 954 99 2489 2579 Fragments arranged by size: 2579 2489 954 99 Bpu10I CC'TnA_GC Cuts at: 3276 3276 Size: 6121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2516 4784 882 Size: 617 1017 2268 2219 Fragments arranged by size: 2268 2219 1017 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2586 3928 4310 4945 Size: 53 83 186 936 1053 1342 382 635 Cuts at: 4945 275 Size: 1451 Fragments arranged by size: 1451 1342 1053 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3084 3114 1178 Size: 30 165 30 1681 30 4185 Fragments arranged by size: 4185 1681 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2158 1186 Size: 972 5149 BseYI C'CCAG_C Cuts at: 2030 5324 5820 2030 Size: 3294 496 2331 Fragments arranged by size: 3294 2331 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5548 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3207 4182 4267 5338 3207 Size: 975 85 1071 3990 Fragments arranged by size: 3990 1071 975 85 BsmI GAATG_Cn' Cuts at: 2330 3193 3632 3725 2330 Size: 863 439 93 4726 Fragments arranged by size: 4726 863 439 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3207 3243 3440 4182 4267 5338 1391 Size: 399 1417 36 197 742 85 1071 2174 Fragments arranged by size: 2174 1417 1071 742 399 197 85 36 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 2092 4010 5740 2092 Size: 1918 1730 2473 Fragments arranged by size: 2473 1918 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2778 3476 844 Size: 1934 698 3489 Fragments arranged by size: 3489 1934 698 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3370 4616 4790 65 Size: 1183 2122 1246 174 1396 Fragments arranged by size: 2122 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2192 794 Size: 1398 4723 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 2986 1184 Size: 1802 4319 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6121 BstYI r'GATC_y Cuts at: 1972 2350 2486 2863 3451 3818 4203 4220 5672 Size: 378 136 377 588 367 385 17 1452 Cuts at: 5672 5758 5770 6116 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 588 385 378 377 367 346 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2870 513 Size: 552 1805 3764 Fragments arranged by size: 3764 1805 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3637 4431 4451 3637 Size: 794 20 5307 Fragments arranged by size: 5307 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3285 3425 3285 Size: 140 5981 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3568 4457 6086 Size: 54 1425 50 275 18 1738 889 1629 Cuts at: 6086 8 Size: 43 Fragments arranged by size: 1738 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2151 2209 2667 3255 4051 1353 Size: 798 58 458 588 796 3423 Fragments arranged by size: 3423 798 796 588 458 58 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2193 2292 4184 4781 5568 5836 1239 Size: 393 561 99 1892 597 787 268 1524 Fragments arranged by size: 1892 1524 787 597 561 393 268 99 EcoRI G'AATT_C Cuts at: 3430 3808 3430 Size: 378 5743 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2059 2091 2147 2179 2666 Size: 32 135 32 487 32 56 32 487 Cuts at: 2666 2698 4762 4794 4836 4868 1373 Size: 32 2064 32 42 32 2626 Fragments arranged by size: 2626 2064 487 487 135 56 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 2663 3720 2663 Size: 1057 5064 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3329 5235 5419 1305 Size: 2024 1906 184 2007 Fragments arranged by size: 2024 2007 1906 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4299 Fragments arranged by size: 4299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4079 4438 4597 518 Size: 648 843 2070 359 159 2042 Fragments arranged by size: 2070 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2312 3537 4214 5362 5607 1606 Size: 424 282 1225 677 1148 245 2120 Fragments arranged by size: 2120 1225 1148 677 424 282 245 NcoI C'CATG_G Cuts at: 513 1065 2870 513 Size: 552 1805 3764 Fragments arranged by size: 3764 1805 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3389 3479 5024 1132 Size: 97 2160 90 1545 2229 Fragments arranged by size: 2229 2160 1545 97 90 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6121 PfoI T'CCnGG_A Cuts at: 2502 2502 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 3017 3691 3017 Size: 674 5447 PstI C_TGCA'G Cuts at: 830 2594 3473 830 Size: 1764 879 3478 Fragments arranged by size: 3478 1764 879 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 2030 2312 2030 Size: 282 5839 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2590 2833 3469 4604 5285 5476 826 Size: 219 1545 243 636 1135 681 191 1471 Fragments arranged by size: 1545 1471 1135 681 636 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 3353 152 Size: 3201 2920 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 3314 4045 5 Size: 47 3262 731 2081 Fragments arranged by size: 3262 2081 731 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6121 StyI C'CwwG_G Cuts at: 513 1065 2870 3560 513 Size: 552 1805 690 3074 Fragments arranged by size: 3074 1805 690 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2022 2974 4148 4165 4318 4503 4948 Size: 1255 442 952 1174 17 153 185 445 Cuts at: 4948 325 Size: 1498 Fragments arranged by size: 1498 1255 1174 952 445 442 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3245 4367 6079 Size: 274 80 33 51 1443 1268 1122 1712 Cuts at: 6079 96 Size: 138 Fragments arranged by size: 1712 1443 1268 1122 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3084 3116 6081 6113 3084 Size: 32 2965 32 3092 Fragments arranged by size: 3092 2965 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2027 2027 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI