Restriction map for
pFN21AE2225
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BcgI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MamI MluI MluNI MroI
MscI MslI MspI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AciI 1264 CCGC CCGC(-3/-1)
AcyI 580 GACGCC G[GA]^CG[CT]C
AseI 1221 ATTAAT AT^TAAT
AsnI 1221 ATTAAT AT^TAAT
AspHI 1198 GTGCTC G[AT]GC[AT]^C
BbsI 965 GTCTTC GAAGAC(2/6)
BbvCI 1270 GCTGAGG CCTCAGC(-5/-2)
BciVI 672 GGATAC GTATCC(6/5)
BglII 345 AGATCT A^GATCT
BpuAI 965 GTCTTC GAAGAC(2/6)
BsaHI 580 GACGCC G[GA]^CG[CT]C
BsaI 505 GGTCTC GGTCTC(1/5)
BsaJI 865 CCATGG C^C[ACGT][ACGT]GG
BseRI 162 CTCCTC GAGGAG(10/8)
BsiHKAI 1198 GTGCTC G[AT]GC[AT]^C
BsmFI 753 GGGAC GGGAC(10/14)
BspHI 87 TCATGA T^CATGA
BspMI 764 ACCTGC ACCTGC(4/8)
BsrDI 1358 GCAATG GCAATG(2/0)
BstAPI 181 GCATTTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 981 GGTGACC G^GT[ACGT]ACC
BstPI 981 GGTGACC G^GT[ACGT]ACC
DraI 1278 TTTAAA TTT^AAA
DsaI 865 CCATGG C^C[GA][CT]GG
EcoO65I 981 GGTGACC G^GT[ACGT]ACC
EcoT14I 865 CCATGG C^C[AT][AT]GG
HgaI 580 GACGC GACGC(5/10)
Hin1I 580 GACGCC G[GA]^CG[CT]C
MfeI 658 CAATTG C^AATTG
MunI 658 CAATTG C^AATTG
NcoI 865 CCATGG C^CATGG
NspI 1380 GCATGT [GA]CATG^[CT]
PshBI 1221 ATTAAT AT^TAAT
PstI 585 CTGCAG CTGCA^G
RcaI 87 TCATGA T^CATGA
SpeI 1348 ACTAGT A^CTAGT
SspI 1307 AATATT AAT^ATT
StyI 865 CCATGG C^C[AT][AT]GG
TspRI 1063 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 15 CCAGTTGTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
BbvI 178 GCTGC GCAGC(8/12)
952 GCTGC
BfaI 725 CTAG C^TAG
1349 CTAG
BmyI 1198 GTGCTC G[AGT]GC[ACT]^C
1234 GTGCCC
BpmI 302 CTCCAG CTGGAG(16/14)
167 CTGGAG
BsmAI 138 GTCTC GTCTC(1/5)
506 GTCTC
BsmI 319 GAATGC GAATGC(1/-1)
1182 GAATGC
Bsp1286I 1198 GTGCTC G[AGT]GC[ACT]^C
1234 GTGCCC
BssKI 498 CCAGG ^CC[ACGT]GG
1339 CCAGG
BstF5I 435 GGATG GGATG(2/0)
477 GGATG
BstNI 498 CCAGG CC^[AT]GG
1339 CCAGG
EcoRII 498 CCAGG ^CC[AT]GG
1339 CCAGG
Fnu4HI 178 GCTGC GC^[ACGT]GC
952 GCTGC
FokI 435 GGATG GGATG(9/13)
477 GGATG
ItaI 178 GCTGC GC^[ACGT]GC
952 GCTGC
MaeI 725 CTAG C^TAG
1349 CTAG
MaeII 1005 ACGT A^CGT
1196 ACGT
MspA1I 23 CAGCTG C[AC]G^C[GT]G
305 CAGCTG
MvaI 498 CCAGG CC^[AT]GG
1339 CCAGG
MwoI 181 GCATTTATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
921 GCAAATAAAGC
PvuII 23 CAGCTG CAG^CTG
305 CAGCTG
ScrFI 498 CCAGG CC^[ACGT]GG
1339 CCAGG
SfaNI 436 GATGC GCATC(5/9)
84 GCATC
SfcI 585 CTGCAG C^T[GA][CT]AG
828 CTATAG
TaqI 264 TCGA T^CGA
1326 TCGA
TfiI 151 GATTC G^A[AT]TC
1108 GATTC
TseI 178 GCTGC G^C[AT]GC
952 GCTGC
TthHB8I 264 TCGA T^CGA
1326 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BsiYI BslI BsrI BstYI Cac8I
DdeI DpnII EarI HinfI HphI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI NdeII NlaIII PleI
RsaI Sau3AI Tru9I Tsp45I Tsp509I
XhoII