(Linear) (Six-base) MAPSORT of: KIEE2225.seq  Check: 2930  from: 1  to: 1416



KIEE2225.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:15  ..

ApoI r'AATT_y

Cuts at:      0     253    1090    1329    1372    1416 
   Size:        253     837     239      43      44

  Fragments arranged by size: 

                 837     253     239      44      43
 
AseI AT'TA_AT

Cuts at:      0    1222    1416 
   Size:       1222     194
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0       1      34     665     698    1416 
   Size:          1      33     631      33     718

  Fragments arranged by size: 

                 718     631      33      33       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     958    1416 
   Size:        958     458
 
BbvCI CC'TCA_GC

Cuts at:      0    1271    1416 
   Size:       1271     145
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     666    1416 
   Size:        666     750
 
BglII A'GATC_T

Cuts at:      0     345    1416 
   Size:        345    1071
 
Bme1580I G_kGCm'C

Cuts at:      0    1238    1416 
   Size:       1238     178
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1074    1153    1416 
   Size:       1074      79     263

  Fragments arranged by size: 

                1074     263      79
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     287    1416 
   Size:        188      99    1129

  Fragments arranged by size: 

                1129     188      99
 
Bpu10I CC'TnA_GC

Cuts at:      0    1271    1416 
   Size:       1271     145
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     511    1416 
   Size:        511     905
 
BsaHI Gr'CG_yC

Cuts at:      0     581    1416 
   Size:        581     835
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1079    1109    1416 
   Size:       1079      30     307

  Fragments arranged by size: 

                1079     307      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     153    1416 
   Size:        153    1263
 
BseYI C'CCAG_C

Cuts at:      0      25    1416 
   Size:         25    1391
 
BsiHKAI G_wGCw'C

Cuts at:      0    1202    1416 
   Size:       1202     214
 
BsmI GAATG_Cn'

Cuts at:      0     325    1188    1416 
   Size:        325     863     228

  Fragments arranged by size: 

                 863     325     228
 
Bsp1286I G_dGCh'C

Cuts at:      0    1202    1238    1416 
   Size:       1202      36     178

  Fragments arranged by size: 

                1202     178      36
 
BspHI T'CATG_A

Cuts at:      0      87    1416 
   Size:         87    1329
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     773    1416 
   Size:        773     643
 
BsrDI GCAATG_nn'

Cuts at:      0    1365    1416 
   Size:       1365      51
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     187    1416 
   Size:        187    1229
 
BstEII G'GTnAC_C

Cuts at:      0     981    1416 
   Size:        981     435
 
BstYI r'GATC_y

Cuts at:      0     345     481     858    1416 
   Size:        345     136     377     558

  Fragments arranged by size: 

                 558     377     345     136
 
BtgI C'CryG_G

Cuts at:      0     865    1416 
   Size:        865     551
 
DraI TTT'AAA

Cuts at:      0    1280    1416 
   Size:       1280     136
 
EarI CTCTTCn'nnn_

Cuts at:      0     146     204     662    1250    1416 
   Size:        146      58     458     588     166

  Fragments arranged by size: 

                 588     458     166     146      58
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     188     287    1416 
   Size:        188      99    1129

  Fragments arranged by size: 

                1129     188      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      54      86     142     174     661     693    1416 
   Size:         54      32      56      32     487      32     723

  Fragments arranged by size: 

                 723     487      56      54      32      32      32
 
MfeI C'AATT_G

Cuts at:      0     658    1416 
   Size:        658     758
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1324    1416 
   Size:       1324      92
 
MspA1I CmG'CkG

Cuts at:      0      25     307    1416 
   Size:         25     282    1109

  Fragments arranged by size: 

                1109     282      25
 
NcoI C'CATG_G

Cuts at:      0     865    1416 
   Size:        865     551
 
NspI r_CATG'y

Cuts at:      0    1384    1416 
   Size:       1384      32
 
PfoI T'CCnGG_A

Cuts at:      0     497    1416 
   Size:        497     919
 
PsiI TTA'TAA

Cuts at:      0    1012    1416 
   Size:       1012     404
 
PstI C_TGCA'G

Cuts at:      0     589    1416 
   Size:        589     827
 
PvuII CAG'CTG

Cuts at:      0      25     307    1416 
   Size:         25     282    1109

  Fragments arranged by size: 

                1109     282      25
 
SfcI C'TryA_G

Cuts at:      0     585     828    1416 
   Size:        585     243     588

  Fragments arranged by size: 

                 588     585     243
 
SpeI A'CTAG_T

Cuts at:      0    1348    1416 
   Size:       1348      68
 
SspI AAT'ATT

Cuts at:      0    1309    1416 
   Size:       1309     107
 
StyI C'CwwG_G

Cuts at:      0     865    1416 
   Size:        865     551
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      17     969    1416 
   Size:         17     952     447

  Fragments arranged by size: 

                 952     447      17
 
TatI w'GTAC_w

Cuts at:      0    1240    1416 
   Size:       1240     176
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1079    1111    1416 
   Size:       1079      32     305

  Fragments arranged by size: 

                1079     305      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      22    1416 
   Size:         22    1394
 

 Enzymes that do cut:

     ApoI     AseI     BaeI     BbsI    BbvCI    BciVI    BglII Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI   BstAPI   BstEII
    BstYI     BtgI     DraI     EarI  Eco57MI    Hin4I     MfeI     MmeI
   MspA1I     NcoI     NspI     PfoI     PsiI     PstI    PvuII     SfcI
     SpeI     SspI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BcgI     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI
    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MscI     MslI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     SwaI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI