AKAP8 ORF clone pFN21AE2056

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Halotag ORF Clone pFN21AE2056

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Clone info.

Species

human

Clone Name

pFN21AE2056 Sequence (with vector) Restriction Map

Product ID

FHC12561

ORF Sequence

DNA sequence (2076 bp)
Protein sequence (692 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

Gene

KIEE2056

Symbol

AKAP8

Alias : AKAP 95, AKAP-8, AKAP-95, AKAP95

Description

A kinase (PRKA) anchor protein 8

Original Clone ID

cs02956

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2076 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 692 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS12329.1 3.3e-185 100.0 AKAP8 CCDS46005.1 3.3e-27 35.4 AKAP8L CCDS77249.1 5.5e-23 35.6 AKAP8L CCDS727.1 6.9e-15 30.7 ZNF326 CCDS81351.1 2.7e-14 30.3 ZNF326 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR007071 390 551 PF04988 A-kinase anchoring protein 95 (AKAP95)
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_005849 692 604692 100.0 100.0 XP_016881630 618 604692 100.0 89.3 XP_011525926 592 604692 99.1 83.4 NP_055186 646 609475 35.4 93.2 XP_005259911 612 609475 34.8 90.3 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AE2056 Download

>KIEE2056  2076 bp
ATGGACCAGGGCTACGGAGGCTACGGGGCGTGGAGTGCTGGACCTGCCAACACCCAGGGT
GCATATGGAACTGGTGTGGCCAGCTGGCAAGGTTATGAAAACTACAATTACTATGGCGCC
CAGAACACCAGTGTCACCACAGGCGCAACCTACAGCTACGGCCCAGCCTCGTGGGAGGCC
GCCAAGGCCAATGATGGCGGCCTGGCGGCCGGGGCCCCTGCCATGCACATGGCCTCTTAC
GGCCCAGAGCCATGCACCGACAATTCCGACTCCCTCATTGCCAAGATCAACCAGCGTTTG
GACATGATGTCCAAGGAAGGAGGCAGGGGCGGGAGCGGCGGCGGTGGGGAGGGCATACAG
GACCGGGAGAGCTCCTTCCGCTTCCAGCCGTTCGAGTCCTATGACTCCAGGCCCTGCCTG
CCGGAGCACAACCCCTACCGCCCCAGCTACAGCTACGACTATGAGTTCGACCTGGGGTCC
GACCGCAATGGCAGCTTTGGGGGGCAGTACAGTGAATGCCGAGACCCAGCCCGGGAGCGG
GGCTCCCTTGATGGCTTCATGCGGGGCCGGGGCCAGGGCCGCTTCCAGGACCGGAGCAAC
CCTGGCACCTTCATGCGCAGCGACCCCTTCGTGCCCCCCGCTGCGTCCTCTGAGCCCCTG
TCCACGCCCTGGAACGAGCTGAACTACGTGGGTGGACGGGGCCTGGGAGGGCCCTCCCCC
AGCCGGCCACCTCCGTCCCTCTTCTCCCAGTCCATGGCTCCCGACTACGGCGTGATGGGC
ATGCAGGGGGCGGGCGGCTATGACAGCACCATGCCCTACGGATGTGGCCGCTCGCAGCCT
CGGATGCGGGATCGGGATCGGCCCAAGAGGAGAGGGTTTGACCGCTTCGGACCAGATGGC
ACGGGCAGGAAACGGAAGCAGTTCCAACTTTACGAGGAGCCAGACACCAAACTGGCCCGG
GTTGACAGTGAAGGAGATTTCTCCGAAAATGATGACGCAGCTGGTGACTTCCGCTCAGGA
GATGAAGAATTCAAGGGTGAGGATGAACTCTGCGACTCTGGGAGGCAAAGAGGAGAGAAG
GAGGACGAGGACGAGGATGTGAAGAAGAGAAGGGAAAAGCAAAGGAGAAGAGACAGGACG
CGGGACCGTGCAGCCGACAGAATTCAGTTTGCCTGTTCTGTATGCAAGTTCCGTAGCTTT
GATGACGAAGAGATCCAGAAGCATCTGCAAAGCAAATTTCACAAAGAGACCCTGCGGTTC
ATAAGCACCAAGCTGCCCGACAAGACCGTGGAGTTCCTCCAGGAATACATTGTAAACAGA
AATAAGAAAATTGAGAAGCGGCGTCAGGAATTGATGGAGAAAGAAACCGCAAAACCAAAA
CCAGATCCTTTCAAAGGGATTGGCCAGGAGCACTTCTTCAAGAAGATCGAGGCTGCTCAC
TGCCTGGCCTGCGACATGCTAATTCCTGCACAGCCGCAGCTCCTCCAGCGGCACCTGCAC
TCCGTGGACCACAATCACAACCGCAGGTTGGCTGCTGAACAGTTCAAGAAAACCAGTCTC
CATGTGGCTAAGAGTGTTTTGAACAACAGACATATAGTGAAGATGCTGGAAAAATACCTC
AAGGGTGAGGACCCTTTCACCAGTGAAACTGTTGATCCAGAAATGGAAGGAGATGACAAT
TTAGGAGGTGAGGATAAGAAAGAGACACCTGAGGAGGTGGCCGCGGACGTCTTAGCAGAG
GTGATTACAGCAGCAGTGAGGGCCGTAGATGGGGAAGGAGCGCCCGCTCCAGAGAGCAGC
GGGGAGCCGGCTGAGGACGAAGGCCCCACGGACACAGCGGAGGCCGGTAGTGATCCTCAA
GCCGAACAGCTGCTGGAAGAGCAGGTGCCCTGTGGAACGGCACATGAGAAGGGCGTCCCC
AAGGCCAGAAGTGAGGCTGCAGAGGCTGGAAATGGCGCCGAGACAATGGCAGCAGAGGCA
GAAAGTGCCCAAACCAGAGTTGCTCCTGCCCCAGCTGCCGCGGATGCTGAAGTGGAACAA
ACTGATGCAGAGTCTAAAGACGCTGTTCCCACAGAA

ORF Protein

Cloned ORF protein sequence for pFN21AE2056 Download

>KIEE2056  692 aa
MDQGYGGYGAWSAGPANTQGAYGTGVASWQGYENYNYYGAQNTSVTTGATYSYGPASWEA
AKANDGGLAAGAPAMHMASYGPEPCTDNSDSLIAKINQRLDMMSKEGGRGGSGGGGEGIQ
DRESSFRFQPFESYDSRPCLPEHNPYRPSYSYDYEFDLGSDRNGSFGGQYSECRDPARER
GSLDGFMRGRGQGRFQDRSNPGTFMRSDPFVPPAASSEPLSTPWNELNYVGGRGLGGPSP
SRPPPSLFSQSMAPDYGVMGMQGAGGYDSTMPYGCGRSQPRMRDRDRPKRRGFDRFGPDG
TGRKRKQFQLYEEPDTKLARVDSEGDFSENDDAAGDFRSGDEEFKGEDELCDSGRQRGEK
EDEDEDVKKRREKQRRRDRTRDRAADRIQFACSVCKFRSFDDEEIQKHLQSKFHKETLRF
ISTKLPDKTVEFLQEYIVNRNKKIEKRRQELMEKETAKPKPDPFKGIGQEHFFKKIEAAH
CLACDMLIPAQPQLLQRHLHSVDHNHNRRLAAEQFKKTSLHVAKSVLNNRHIVKMLEKYL
KGEDPFTSETVDPEMEGDDNLGGEDKKETPEEVAADVLAEVITAAVRAVDGEGAPAPESS
GEPAEDEGPTDTAEAGSDPQAEQLLEEQVPCGTAHEKGVPKARSEAAEAGNGAETMAAEA
ESAQTRVAPAPAAADAEVEQTDAESKDAVPTE

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AE2056 Download

>pFN21AE2056  6781 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGGACCAGGGCTACGGAGGCTACGGGGCGTGGAG
TGCTGGACCTGCCAACACCCAGGGTGCATATGGAACTGGTGTGGCCAGCTGGCAAGGTTA
TGAAAACTACAATTACTATGGCGCCCAGAACACCAGTGTCACCACAGGCGCAACCTACAG
CTACGGCCCAGCCTCGTGGGAGGCCGCCAAGGCCAATGATGGCGGCCTGGCGGCCGGGGC
CCCTGCCATGCACATGGCCTCTTACGGCCCAGAGCCATGCACCGACAATTCCGACTCCCT
CATTGCCAAGATCAACCAGCGTTTGGACATGATGTCCAAGGAAGGAGGCAGGGGCGGGAG
CGGCGGCGGTGGGGAGGGCATACAGGACCGGGAGAGCTCCTTCCGCTTCCAGCCGTTCGA
GTCCTATGACTCCAGGCCCTGCCTGCCGGAGCACAACCCCTACCGCCCCAGCTACAGCTA
CGACTATGAGTTCGACCTGGGGTCCGACCGCAATGGCAGCTTTGGGGGGCAGTACAGTGA
ATGCCGAGACCCAGCCCGGGAGCGGGGCTCCCTTGATGGCTTCATGCGGGGCCGGGGCCA
GGGCCGCTTCCAGGACCGGAGCAACCCTGGCACCTTCATGCGCAGCGACCCCTTCGTGCC
CCCCGCTGCGTCCTCTGAGCCCCTGTCCACGCCCTGGAACGAGCTGAACTACGTGGGTGG
ACGGGGCCTGGGAGGGCCCTCCCCCAGCCGGCCACCTCCGTCCCTCTTCTCCCAGTCCAT
GGCTCCCGACTACGGCGTGATGGGCATGCAGGGGGCGGGCGGCTATGACAGCACCATGCC
CTACGGATGTGGCCGCTCGCAGCCTCGGATGCGGGATCGGGATCGGCCCAAGAGGAGAGG
GTTTGACCGCTTCGGACCAGATGGCACGGGCAGGAAACGGAAGCAGTTCCAACTTTACGA
GGAGCCAGACACCAAACTGGCCCGGGTTGACAGTGAAGGAGATTTCTCCGAAAATGATGA
CGCAGCTGGTGACTTCCGCTCAGGAGATGAAGAATTCAAGGGTGAGGATGAACTCTGCGA
CTCTGGGAGGCAAAGAGGAGAGAAGGAGGACGAGGACGAGGATGTGAAGAAGAGAAGGGA
AAAGCAAAGGAGAAGAGACAGGACGCGGGACCGTGCAGCCGACAGAATTCAGTTTGCCTG
TTCTGTATGCAAGTTCCGTAGCTTTGATGACGAAGAGATCCAGAAGCATCTGCAAAGCAA
ATTTCACAAAGAGACCCTGCGGTTCATAAGCACCAAGCTGCCCGACAAGACCGTGGAGTT
CCTCCAGGAATACATTGTAAACAGAAATAAGAAAATTGAGAAGCGGCGTCAGGAATTGAT
GGAGAAAGAAACCGCAAAACCAAAACCAGATCCTTTCAAAGGGATTGGCCAGGAGCACTT
CTTCAAGAAGATCGAGGCTGCTCACTGCCTGGCCTGCGACATGCTAATTCCTGCACAGCC
GCAGCTCCTCCAGCGGCACCTGCACTCCGTGGACCACAATCACAACCGCAGGTTGGCTGC
TGAACAGTTCAAGAAAACCAGTCTCCATGTGGCTAAGAGTGTTTTGAACAACAGACATAT
AGTGAAGATGCTGGAAAAATACCTCAAGGGTGAGGACCCTTTCACCAGTGAAACTGTTGA
TCCAGAAATGGAAGGAGATGACAATTTAGGAGGTGAGGATAAGAAAGAGACACCTGAGGA
GGTGGCCGCGGACGTCTTAGCAGAGGTGATTACAGCAGCAGTGAGGGCCGTAGATGGGGA
AGGAGCGCCCGCTCCAGAGAGCAGCGGGGAGCCGGCTGAGGACGAAGGCCCCACGGACAC
AGCGGAGGCCGGTAGTGATCCTCAAGCCGAACAGCTGCTGGAAGAGCAGGTGCCCTGTGG
AACGGCACATGAGAAGGGCGTCCCCAAGGCCAGAAGTGAGGCTGCAGAGGCTGGAAATGG
CGCCGAGACAATGGCAGCAGAGGCAGAAAGTGCCCAAACCAGAGTTGCTCCTGCCCCAGC
TGCCGCGGATGCTGAAGTGGAACAAACTGATGCAGAGTCTAAAGACGCTGTTCCCACAGA
AGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGC
AAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTG
AGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATT
GATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATT
TGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAAC
AATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAG
TAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTT
TCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGA
CTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGC
GGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTC
CGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAA
ACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAA
CTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATG
ATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAA
GAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTC
ACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTA
ACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAG
CTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACA
ACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATA
GACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGC
TGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCA
CTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGG
TAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAA
TACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGC
AAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCC
CTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTAT
AAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCT
CACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACG
AACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACC
CGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGA
GGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAA
GGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTA
GCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTG
ACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGA
TCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCG
CTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGC
CTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGC
AGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGC
GTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTA
GCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATC
T